Asian Pacific Journal of Cancer Prevention

  • 제17권11호
  • /
  • Pages.4907-4911
  • /
  • 2016
  • /
  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of JC and Cytomegalo Viruses in Glioblastoma Tissue

  • Afshar, Reza Malekpour (Research Center for Tropical and Infectious Disease) ;
  • Mollaei, Hamid Reza (Department of Medical Microbiology, Kerman University of Medical Sciences) ;
  • Zandi, Bahare (Research Center for Tropical and Infectious Disease) ;
  • Iranpour, Maryam (Department of Pathology and Stem Cell Research Center, Kerman University of Medical Sciences)
  • 발행 : 2016.11.01
⟨ 이전 논문 다음 논문 ⟩

초록

Glioblastoma multiforme (GBM) is the most aggressive of the gliomas, a collection of tumors arising from glia in the central nervous system. Possible associations between the human cytomegalovirus (HCMV) and the JC virus with GBM are now attracting interest. Our present aim was to investigate the prevalence of the two viruses in Iranian patients from Kerman's cities in the south of Iran. In addition, the expression rates of pp65, large T antigen and p53 proteins were assessed and their relation with GBM evaluated using reverse transcription real time PCR (rReal Time PCR). A total of 199 patients with GBM cancer were enrolled, with $mean{\pm}SD$ ages of $50.0{\pm}19.5$ and $50.7{\pm}19.6$ years for males and females, respectively. The P53 rate was dramatically low suggesting an aetiological role,. Large T antigen expression was found in JC positive samples, while the PP65 antigen was observed in patients positive for CMV and JC. HCMV products and JC virus with oncogenic potential may induce the development of various tumors including glioblastomas. The JC virus produces an early gene product, T-antigen, which has the ability to associate with and functionally inactivate well-studied tumor suppressor proteins including p53 and pRB.

키워드

참고문헌

  1. Alibek K, Kakpenova A, Baiken Y (2013). Role of infectious agents in the carcinogenesis of brain and head and neck cancers. Infect Agent Cancer, 8, 7. https://doi.org/10.1186/1750-9378-8-7
  2. Cobbs CS (2011). Evolving evidence implicates cytomegalovirus as a promoter of malignant glioma pathogenesis. Herpesviridae, 2, 10. https://doi.org/10.1186/2042-4280-2-10
  3. Costa B, Bendinelli S, Gabelloni P, et al (2013). Human glioblastoma multiforme: p53 reactivation by a novel MDM2 inhibitor. PLoS One, 8, e72281. https://doi.org/10.1371/journal.pone.0072281
  4. Del Valle L, Azizi SA, Krynska B, et al (2000). Reactivation of human neurotropic JC virus expressing oncogenic protein in a recurrent glioblastoma multiforme. Ann Neurol, 48, 932-6. https://doi.org/10.1002/1531-8249(200012)48:6<932::AID-ANA15>3.0.CO;2-E
  5. Del Valle L, Delbue S, Gordon J, et al (2002). Expression of JC virus T-antigen in a patient with MS and glioblastoma multiforme. Neurology, 58, 895-900. https://doi.org/10.1212/WNL.58.6.895
  6. Devireddy LR, Kumar KU, Pater MM, et al (1996). Evidence for a mechanism of demyelination by human JC virus: negative transcriptional regulation of RNA and protein levels from myelin basic protein gene by large tumor antigen in human glioblastoma cells. J Med Virol, 49, 205-11. https://doi.org/10.1002/(SICI)1096-9071(199607)49:3<205::AID-JMV8>3.0.CO;2-8
  7. Djuzenova CS, Fiedler V, Memmel S, et al (2015). Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in PTEN and p53 status. Exp Cell Res, 330, 346-57. https://doi.org/10.1016/j.yexcr.2014.08.013
  8. Dos Santos CJ, Stangherlin LM, Figueiredo EG, et al (2014). High prevalence of HCMV and viral load in tumor tissues and peripheral blood of glioblastoma multiforme patients. J Med Virol, 86, 1953-61. https://doi.org/10.1002/jmv.23820
  9. Ghazi A, Ashoori A, Hanley PJ, et al (2012). Generation of polyclonal CMV-specific T cells for the adoptive immunotherapy of glioblastoma. J Immunother, 35, 159-68. https://doi.org/10.1097/CJI.0b013e318247642f
  10. Knight A, Arnouk H, Britt W, et al (2013). CMV-independent lysis of glioblastoma by ex vivo expanded/activated Vdelta1+ gammadelta T cells. PLoS One, 8, e68729. https://doi.org/10.1371/journal.pone.0068729
  11. Lehrer S, Green S, Ramanathan L, et al (2012). No consistent relationship of glioblastoma incidence and cytomegalovirus seropositivity in whites, blacks, and Hispanics. Anticancer Res, 32, 1113-5.
  12. Lucas KG, Bao L, Bruggeman R, et al (2011). The detection of CMV pp65 and IE1 in glioblastoma multiforme. J Neurooncol, 103, 231-8. https://doi.org/10.1007/s11060-010-0383-6
  13. Major EO, Mourrain P, Cummins C (1984). JC virus-induced owl monkey glioblastoma cells in culture: biological properties associated with the viral early gene product. Virol J, 136, 359-67. https://doi.org/10.1016/0042-6822(84)90172-7
  14. Matlaf LA, Harkins LE, Bezrookove V, et al (2013). Cytomegalovirus pp71 protein is expressed in human glioblastoma and promotes pro-angiogenic signaling by activation of stem cell factor. PLoS One, 8, e68176. https://doi.org/10.1371/journal.pone.0068176
  15. Nair SK, De Leon G, Boczkowski D, et al (2014). Recognition and killing of autologous, primary glioblastoma tumor cells by human cytomegalovirus pp65-specific cytotoxic T cells. Clin Cancer Res, 20, 2684-94. https://doi.org/10.1158/1078-0432.CCR-13-3268
  16. Ottenhausen M, Bodhinayake I, Schaefer PM, et al (2014). VIGAS and beyond: the impact of HCMV-infection and its treatment in glioblastoma. J Neurosurg, 74, 23-4. https://doi.org/10.1227/01.neu.0000442982.91829.da
  17. Pina-Oviedo S, De Leon-Bojorge B, Cuesta-Mejias T, et al (2006). Glioblastoma multiforme with small cell neuronallike component: association with human neurotropic JC virus. Acta Neuropathol, 111, 388-96. https://doi.org/10.1007/s00401-006-0050-3
  18. Poch E, Minambres R, Mocholi E, et al (2007). RhoE interferes with Rb inactivation and regulates the proliferation and survival of the U87 human glioblastoma cell line. Exp Cell Res, 313, 719-31. https://doi.org/10.1016/j.yexcr.2006.11.006
  19. Price RL, Song J, Bingmer K, et al (2013). Cytomegalovirus contributes to glioblastoma in the context of tumor suppressor mutations. Cancer Res, 73, 3441-50. https://doi.org/10.1158/0008-5472.CAN-12-3846
  20. Sabbatino F, Fusciello C, Somma D, et al (2014). Effect of p53 activity on the sensitivity of human glioblastoma cells to PARP-1 inhibitor in combination with topoisomerase I inhibitor or radiation. Cytometry A, 85, 953-61. https://doi.org/10.1002/cyto.a.22563
  21. Scheurer ME, Bondy ML, Aldape KD, et al (2008). Detection of human cytomegalovirus in different histological types of gliomas. Acta Neuropathol, 116, 79-86. https://doi.org/10.1007/s00401-008-0359-1
  22. Tognon M, Casalone R, Martini F, et al (1996). Large T antigen coding sequences of two DNA tumor viruses, BK and SV40, and nonrandom chromosome changes in two glioblastoma cell lines. Cancer Genet Cytogenet, 90, 17-23. https://doi.org/10.1016/0165-4608(96)00067-2
  23. Zhang W, Fulci G, Wakimoto H, et al (2013). Combination of oncolytic herpes simplex viruses armed with angiostatin and IL-12 enhances antitumor efficacy in human glioblastoma models. Neoplasia, 15, 591-9. https://doi.org/10.1593/neo.13158