Asian Pacific Journal of Cancer Prevention

  • 제17권11호
  • /
  • Pages.4929-4934
  • /
  • 2016
  • /
  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
권호 표지
DOI QR코드

DOI QR Code

Nano-Micelle of Moringa Oleifera Seed Oil Triggers Mitochondrial Cancer Cell Apoptosis

  • Abd-Rabou, Ahmed A (Hormones Department, Medical Research Division, National Research Centre) ;
  • Zoheir, Khairy M A (Cell Biology Department, National Research Centre) ;
  • Kishta, Mohamed S (Hormones Department, Medical Research Division, National Research Centre) ;
  • Shalby, Aziza B (Hormones Department, Medical Research Division, National Research Centre) ;
  • Ezzo, Mohamed I (Horticulture Technology Department, National Research Centre)
  • 발행 : 2016.11.01
⟨ 이전 논문 다음 논문 ⟩

초록

Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the commercially available chemotherapies, natural products-loaded nano-composites are recommended to improve cancer targetability and decrease the harmful impact on normal cells. This study aimed at exploring the anti-cancer impacts of Moringa oleifera seed oil in its free- (MO) and nano-formulations (MOn) through studying whether it mechanistically promotes mitochondrial apoptosis-mediating cell death. Mitochondrial-based cytotoxicity and flow cytometric-based apoptosis analyses were performed on cancer HepG2, MCF7, HCT 116, and Caco-2 cell lines against normal kidney BHK-21 cell line. The present study resulted that MOn triggered colorectal cancer Caco-2 and HCT 116 cytotoxicity via mitochondrial dysfunction more powerful than its free counterpart (MO). On the other side, MOn and MO remarkably induces HCT 116 mitochondrial apoptosis, while sparing normal BHK-21 cells with minimal cytotoxic effect. The present results concluded that nano-micelle of Moringa oleifera seed oil (MOn) can provide a novel therapeutic approach for colorectal and breast cancers via mitochondrial-mediated apoptosis, while sparing normal and even liver cancer cells a bit healthy or with minimal harmful effect. Intriguingly, MOn induced breast cancer not hepatocellular carcinoma cell death.

키워드

과제정보

연구 과제번호 : Recent approaches in the utilization of Moringa oleifera and Moringa peregrina as a good nutritional, medicinal and industrial plant in Egypt

연구 과제 주관 기관 : Science and Technology Development Fund (STDF)

참고문헌

  1. Abd-Rabou AA, Zoheir K, Ahmed HH (2012). Potential impact of curcumin and taurine on human hepatoma cells using huh-7 cell line. Clin Biochem, 45, 1519-21. https://doi.org/10.1016/j.clinbiochem.2012.06.032
  2. Abd-Rabou AA (2016). Calcium, a cell cycle commander, drives colon cancer cell diffpoptosis. Ind J Clin Biochem, 1-10.
  3. Abdulkarim SM, Lai OM, Muhammad SKS, Long K, Ghazali HM (2005). Some physic-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem, 93, 253-63. https://doi.org/10.1016/j.foodchem.2004.09.023
  4. Ahmad MB, Rauf A, Osman MJ (1989). Oil technologies'. Association of India, 3, 46-7.
  5. Ahmed HH, Abd-Rabou AA, Hassan AZ, Kotob SE (2015). Phytochemical analysis and anti-cancer investigation of boswellia serrata bioactive constituents in vitro. Asian Pac J Cancer Prev, 16,7179-88 https://doi.org/10.7314/APJCP.2015.16.16.7179
  6. Al-Kuraya KS, Bavi PP, Ezzat AA (2006). Colorectal carcinoma from Saudi Arabia: Analysis of MLH-1, MSH- 2 and p53 genes by immunohistochemistry and tissue microarray analysis. Saudi Med J, 27, 323-8.
  7. Al-Oqail MM, Farshori NN, Al-Sheddi ES (2013). In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell cines. Asian Pac J Cancer Prev, 14, 1829-32. https://doi.org/10.7314/APJCP.2013.14.3.1829
  8. Anwar F, Latif S, Ashrat M (2007). M. oleifera: A food plant with multiple medicinal uses. Phytother Res, 21, 17-25. https://doi.org/10.1002/ptr.2023
  9. Asare GA, Gyan B, Bugyei K, Adjei S (2012). Toxicity potentials of the nutraceutical Moringa oleifera at suprasupplementation levels. J Ethnopharmacol, 139, 265-72. https://doi.org/10.1016/j.jep.2011.11.009
  10. Awodele O, Oreagbaa IA, Odomaa S, Teixeira da Silva JA, Osunkaluc VO (2012). Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol, 139, 330-6. https://doi.org/10.1016/j.jep.2011.10.008
  11. Chuang PH, Lee CW, Chou JC (2007). Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam. Bioresour Technol, 98, 232-6. https://doi.org/10.1016/j.biortech.2005.11.003
  12. Cragg GM, Newman DJ (2005). Plant as source of anticancer agents. J Ethnopharmacol, 100, 72-9. https://doi.org/10.1016/j.jep.2005.05.011
  13. De Martel C, Ferlay J, Franceschi S (2012). Global burden of cancers attributable to infections in 2008: a review and synthetic analysis. Lanc Oncol, 13, 607-15. https://doi.org/10.1016/S1470-2045(12)70137-7
  14. Fahey JW (2005). Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1. Trees for Life J, 1, 1-33.
  15. Farooq M, Hozzein WN, Elsayed EA (2013). Identification of histone deacetylase I protein complexes in liver cancer cells. Asian Pac J Cancer Prev, 14, 915-21. https://doi.org/10.7314/APJCP.2013.14.2.915
  16. Ferlay J, Soerjomataram I, Dikshit R, (2014). Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN. Inter J Cancer, 136, 359-86.
  17. Ghazali HM, Mohammed AS (2011). Moringa (Moringa oleifera) seed oil: composition, nutritional aspects and health attributes. in 'nuts & seeds in health and disease prevention', Eds. Preedy V, Watson R and Patel V. Elsevier Academic Press, USA, 787-93.
  18. Heo BG, Park YJ, Park YS (2014). Anticancer and antioxidant effects of extracts from different parts of indigo plant. Indust Crops Products, 56, 9-16. https://doi.org/10.1016/j.indcrop.2014.02.023
  19. Huang YJ, Zhang YY, Liu G (2014). Preliminary evaluation of the in vitro efficacy of 1, 2-di(quinazolin-4- yl) diselane against SiHa cervical cancer cells. Asian Pac J Cancer Prev, 15, 6301-6. https://doi.org/10.7314/APJCP.2014.15.15.6301
  20. Hussein MA, Gobba NA, El Bishbishy MH (2014). Composition, in vitro antioxidant and antitumor properties of essential oil from the seeds of Moringa oleifera. Inter J Pharm Scien, 4, 532-40.
  21. Iqbal S, Bhanger MI (2006). Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J Food Comp Anal, 19, 544-51. https://doi.org/10.1016/j.jfca.2005.05.001
  22. Janick J, Paull RE (2008). The encyclopedia of fruit and nuts. CABI, Cambridge, UK, 509-10.
  23. Kumar B, Jain SK, Prajapati SK (2011). Effect of penetration enhancer DMSO on in-vitro skin permeation of acyclovir transdermal microemulsion formulation. Inter J Drug Deliv, 83-94
  24. Laandrault N, Pouchert P, Ravel P (2001). Antioxidant activities and phenolic level of French wines from different varieties and vintages. J Agric Food Chem, 49, 3341-3. https://doi.org/10.1021/jf010128f
  25. Lalas S, Tsaknis J (2002). Extraction and identification of natural Antioxidant from the seeds of the Moringa oleifera tree variety of Malaw. J Am Oil Chem Soc, 79, 677-83. https://doi.org/10.1007/s11746-002-0542-2
  26. Manzoor M, Anwar F, Iqball T, Bhanger MI (2007). Physico-chemical characterization of Moringa concanensis seeds and seed oil. J Am Oil Chem Soc, 84, 413-9. https://doi.org/10.1007/s11746-007-1055-3
  27. Mustafa J, Khan SI, Ma G, Walker LS, Khana IA (2004). Synthesis and anticancer activities of fatty acid analogs of podophyllotoxin. Lipids, 39, 167-72. https://doi.org/10.1007/s11745-004-1215-5
  28. Park JH, Lee S, Kim JH (2008). Polymeric nanomedicine for cancer therapy. Prog Polym Sci, 33, 113-37. https://doi.org/10.1016/j.progpolymsci.2007.09.003
  29. Pritchard JLR (1991). Analysis and properties of oilseeds. In: Analysis of Oilseeds, Fats and Fatty Foods, (Rossell J.B., Pritchard, J.L.R., Ed.) 80-98,127. Elsevier Applied science, London, New York.
  30. Rao KS, Mishra SH (1998). Antiinflammatory and hepatoprotective activities of fruits of Moriga pterygosperma gaertn. Ind J Nat Prod, 14, 23-6.
  31. Rashid U, Anwar F, Moser BR, Knothe G (2008). Moringa oleifera: A possible source for biodiesel. Bioresour Technol, 99, 8175-9. https://doi.org/10.1016/j.biortech.2008.03.066
  32. Ravichandran D, Johnson CD (1999). Anticancer effects of essential fatty acids. in 'pancreatic disease', Eds. Johnson CD. Springer-Verlag, London 325-37.
  33. Sanchez-Machado DI, Nun ez JA, Reyes MC (2010). Nutritional quality of edible parts of Moringa oleifera. Food Anal Methods, 3, 175-80. https://doi.org/10.1007/s12161-009-9106-z
  34. Scheim DE (2009). Cytotoxicity of unsaturated fatty acids in fresh human tumor explants: concentration thresholds and implications for clinical efficacy. Lipids Health Dis, 8, 54-9. https://doi.org/10.1186/1476-511X-8-54
  35. Sharaf-Eldin E , Asian M (2015). In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines. Asian Pac J Cancer Prev, 16, 4671-5 https://doi.org/10.7314/APJCP.2015.16.11.4671
  36. Siddhuraju P, Becker K (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agric Food Chem, 51, 2144-55. https://doi.org/10.1021/jf020444+
  37. Sultana S, Asif HM, Nazar HMI (2014). Medicinal plants combating against cancer-a green anticancer approach. Asian Pac J Cancer Prev, 15, 4385-94. https://doi.org/10.7314/APJCP.2014.15.11.4385
  38. Tatiana M, Filomena N, Emilia M, Florinda F (2013). Chemical omposition and Biological Activity of the Essential Oil from Leaves of Moringa oleifera Lam. Cultivated in Mozambique. Molecules, 18, 10989-1000. https://doi.org/10.3390/molecules180910989
  39. Van Meerloo J, Kaspers GJ, Cloos J (2011). Cell sensitivity assays: the MTT assay. Methods Mol Biol, 731, 237-45.
  40. WHO Cancer Updated February (2015). Fact sheet $N^{\circ}$297 http://www.who.int/mediacentre/factsheets/fs297/en/