Asian Pacific Journal of Cancer Prevention

  • 제17권11호
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  • Pages.4965-4971
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  • 2016
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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DOI QR코드

DOI QR Code

Inhibitory Effect of Ginseng on Breast Cancer Cell Line Growth Via Up-Regulation of Cyclin Dependent Kinase Inhibitor, p21 and p53

  • Shabanah, Othman A AL (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University) ;
  • Alotaibi, Moureq R (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University) ;
  • Rejaie, Salim S Al (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University) ;
  • Alhoshani, Ali R (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University) ;
  • Almutairi, Mashal M (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University) ;
  • Alshammari, Musaad A (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University) ;
  • Hafez, Mohamed M (College of Pharmacy, Pharmacology and Toxicology Department, Kind Saud University)
  • 발행 : 2016.11.01
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: Breast cancer is global female health problem worldwide. Most of the currently used agents for breast cancer treatment have toxic side-effects. Ginseng root, an oriental medicine, has many health benefits and may exhibit direct anti-cancer properties. This study was performed to assess the effects of ginseng on breast cancer cell lines. Materials and Methods: Cytotoxicity of ginseng extract was measured by MTT assay after exposure of MDA-MB-231, MCF-10A and MCF-7 breast cancer cells to concentrations of 0.25, 0.5, 1, 1.5, 2 and 2.5 mg/well. Expression levels of p21WAF, p16INK4A, Bcl-2, Bax and P53 genes were analyzed by quantitative real time PCR. Results: The treatment resulted in inhibition of cell proliferation in a dose-and time-dependent manner. p53, p21WAF1and p16INK4A expression levels were up-regulated in ginseng treated MDA-MB-231 and MCF-7 cancer cells compared to untreated controls and in MCF-10A cells. The expression levels of Bcl2 in the MDA-MB-231 and MCF-7 cells were down-regulated. In contrast, that of Bax was significantly up-regulated. Conclusion: The results of this study revealed that ginseng may inhibit breast cancer cell growth by activation of the apoptotic pathway.

키워드

참고문헌

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