Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Characterization of Lactobacillus reuteri BCLR-42 and Lactobacillus plantarum BCLP-51 as novel dog probiotics with innate immune enhancing properties

  • Kim, Eun Jin (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Kang, Yeong Im (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Bang, Tae Il (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Myoung Han (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Sang Won (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Choi, In Soo (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Song, Chang Seon (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Joong Bok (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University) ;
  • Park, Seung Yong (Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University)
  • Received : 2016.01.15
  • Accepted : 2016.04.26
  • Published : 2016.06.30
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Abstract

Probiotics that are able to provide beneficial effects on animal health have become important ingredients of dog foods. This study was conducted to characterize the probiotic potentials of two strains, Lactobacillus reuteri BCLR-42 and Lactobacillus plantarum BCLP-51, that were derived from feces of healthy dogs and evaluated based on tolerance to low pH and bile acid, antimicrobial activities, enzyme profiles, sensitivity to antibiotics, and innate immune enhancing potentials. Both strains showed survival of more than 90% at pH 3 and 0.2% bile acid and exhibited broad antimicrobial activities against indicator bacteria. Moreover, both strains showed high sensitivity to antibiotics, except vancomycin, metronidazole, and gentamicin. The alkaline phosphatase was negligible (score 0), whereas they showed strong beta galactosidase activity (score range 5 or 3, respectively). The phagocytosis and oxidative burst activities of canine granulocytes were significantly enhanced in response to both strains. These results show that both strains have the capability to act as probiotics and the potential for application as ingredients in dog foods.

Keywords

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