Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (-)-Loliolide by HPLC

  • Cho, Sunghun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Jung, Yong-Su (Yeong-Gwang Agricultural Technology Center) ;
  • Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2015.12.21
  • Accepted : 2016.01.30
  • Published : 2016.06.30
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Abstract

Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as ${\beta}$-sitosterol, (-)-loliolide, rutin, and pyrimidinedione by MS, $^1H$-, and $^{13}C$-NMR spectroscopic analysis. Among them, (-)-loliolide was isolated for the first time from B. nivea. A content analysis of (-)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (-)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.

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