Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and α-Glucosidase Inhibitory Activities of Tradescantia pallida (Rose) Hunt Leaf Extract and Fractions

팔리다자주닭개비 잎 추출물 및 분획물의 항산화 및 α-글루코시다아제 저해 활성

  • Kim, Ju Sung (Majors in Plant Resource and Environment, College of Agriculture and Life Sciences, SARI, Jeju National University) ;
  • Kim, Kyeoung Cheol (Majors in Plant Resource and Environment, College of Agriculture and Life Sciences, SARI, Jeju National University)
  • 김주성 (제주대학교 생명자원과학대학 친환경농업연구소) ;
  • 김경철 (제주대학교 생명자원과학대학 친환경농업연구소)
  • Received : 2016.04.25
  • Accepted : 2016.06.08
  • Published : 2016.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: The biological activities of Tradescantia pallida grown in Korea have not been well determined, thus the aim of this study was to investigate the possibility of using it as a medicinal plant. Methods and Results: To investigate the antioxidant activity, ${\alpha}$-glucosidase inhibitory effect and antimicrobial activity of T. pallida, we performed the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power assay. This assay for T. pallida leaf extract showed the highest antioxidant activity for the ethyl acetate fraction ($RC_{50}=14.55{\pm}0.16{\mu}g/m{\ell}$ and Abs = 0.613 at $300{\mu}g$). Further, the ethyl acetate fraction exhibited higher ${\alpha}$-glucosidase inhibitory effect with an $IC_{50}$ value of $14.1{\pm}0.1{\mu}g/m{\ell}$ and showed antimicrobial activity against gram positive bacteria (minimum inhibitory concentration = $1,000{\mu}g/m{\ell}$). Conclusions: The ethyl acetate fraction of the crude methanol extract of T. pallida showed remarkable antioxidant activity, ${\alpha}$-glucosidase inhibitory effects and antimicrobial activity. These activities might be related to the flavonoid content in the T. pallida leaf extract.

Keywords

References

  1. Awad NE, Abdelkawy MA, Hamed MA, Souleman AMA, Abdelrahman EH and Ramadan NS. (2015). Antioxidant and hepatoprotective effects of Justicia spicigera ethyl acetate fraction and characterization of its anthocyanin content. International Journal of Pharmacy and Pharmaceutical Sciences. 7:91-96.
  2. Blois MS. (1958). Antioxidant determination by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  3. Brand-Williams W, Cuvelier ME and Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology. 28:25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  4. Chalker-Scott L. (1999). Environmental significance of anthocyanins in plant stress responses. Photochemistry and Photobiology. 70:1-9. https://doi.org/10.1111/j.1751-1097.1999.tb01944.x
  5. Chimpan C and Sipos M. (2009). Anatomy of the vegetative organs of Tradescantia pallida purpurea. Biharean Biologist. 3:1-4.
  6. Dai J and Mumper RJ. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. 15:7313-7352. https://doi.org/10.3390/molecules15107313
  7. Gabrielska J, Oszmianski J, Komorowska M and Langner M. (1999). Anthocyanin extracts with antioxidant and radical scavenging effect. Zeitschrift fur Naturforschung C, A Journal of Bioscience. 54:319-324.
  8. Hertog MGL, Hollman PCH and van de Putte B. (1993). Content of potentially anticarcinogenic flavonoids of tea infusions, wines, and fruit juices. Journal of Agricultural and Food Chemistry. 41:1242-1246. https://doi.org/10.1021/jf00032a015
  9. Hunt DR. (1980). Sections and series in Tradescantia: American Commelinaceae: IX. Kew Bulletin. 35:437-442. https://doi.org/10.2307/4114596
  10. Hyun TK, Kim HC and Kim JS. (2014). Antioxidant and antidiabetic activity of Thymus quinquecostatus Celak. Industrial Crops and Products. 52:611-616. https://doi.org/10.1016/j.indcrop.2013.11.039
  11. Hyun TK, Kim HC and Kim JS. (2015). In vitro screening for antioxidant, antimicrobial, and antidiabetic properties of some Korean native plants on Mt. Halla, Jeju Island. Indian Journal of Pharmaceutical Sciences. 77:668-674. https://doi.org/10.4103/0250-474X.174984
  12. Jeong HJ, Kim JS, Sa YJ, Kim MO, Yang J and Kim MJ. (2011). Antioxidant activity and ${\alpha}$-glucosidase inhibitory effect of Jerusalem artichoke(Helianthus tuberosus) methanol extracts by heat treatment conditions. Korean Journal of Medicinal Crop Science. 19:257-263. https://doi.org/10.7783/KJMCS.2011.19.4.257
  13. Kim EJ, Choi JY, Yu M, Kim MY, Lee S and Lee BH. (2012). Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean Journal of Food Science and Technology. 44:337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  14. Kim JS. (2014). Antioxidant, ${\alpha}$-glucosidase inhibitory and antimicrobial activities of extracts from Maesa japonica (Thunb.). Korean Journal of Medicinal Crop Science. 22:289-294. https://doi.org/10.7783/KJMCS.2014.22.4.289
  15. Kim JS, Hyun TK and Kim MJ. (2011b). The inhibitory effects of ethanol extracts from sorghum, foxtail millet and proso millet on ${\alpha}$-glucosidase and ${\alpha}$-amylase activities. Food Chemistry. 124:1647-1651. https://doi.org/10.1016/j.foodchem.2010.08.020
  16. Kim JS, Kwon YS, Chun WJ, Kim TY, Sun J, Yu CY and Kim MJ. (2010). Rhus verniciflua Stokes flavonoid extracts have anti-oxidant, anti-microbial and ${\alpha}$-glucosidase inhibitory effect. Food Chemistry. 120:539-543. https://doi.org/10.1016/j.foodchem.2009.10.051
  17. Kim JS, Kwon YS, Sa YJ and Kim MJ. (2011a). Isolation and identification of sea buckthorn(Hippophae rhamnoides) phenolics with antioxidant activity and ${\alpha}$-glucosidase inhibitory effect. Journal of Agricultural and Food Chemistry. 59:138-144. https://doi.org/10.1021/jf103130a
  18. Li TSC. (2006). Taiwanese native medicinal plant: Phytopharmacology and therapeutic values. CRC Taylor and Francis. Boca Raton. Florida, USA. p.1-328.
  19. Liu RH. (2004). Potential synergy of phytochemicals in cancer prevention: Mechanism of action. The Journal of Nutrition. 134:3479S-3485S. https://doi.org/10.1093/jn/134.12.3479S
  20. Middleton E and Kandaswami C. (1994). Potential health-promoting properties of citrus flavonoids. Food Technology. 48:115-119.
  21. Misik M, Ma TH, Nersesyan A, Monarca S, Kim JK and Knasmueller S. (2011). Micronucleus assays with Tradescantia pollen tetrads: An update. Mutagenesis. 26:215-221. https://doi.org/10.1093/mutage/geq080
  22. Molyneux P. (2004). The use of the stable free radical diphenylpicrylhydrazyl(DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology. 26:211-219.
  23. Oyaizu M. (1986). Studies on products of browning reaction: Antioxidative activity of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 44:307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  24. Paiva EAS, Isaias RMdS, Vale FHA and Queiroz CGdS. (2003). The influence of light intensity on anatomical structure and pigment contents of Tradescantia pallida(Rose) Hunt. cv. purpurea Boom(Commelinaceae) leaves. Brazilian Archives of Biology and Technology. 46:617-624. https://doi.org/10.1590/S1516-89132003000400017
  25. Rice-Evans C, Miller N and Paganga G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science. 2:152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  26. Ryu SW, Jin CW, Lee HS, Lee JY, Sapkota K, Lee BG, Yu CY, Lee MK, Kim MJ and Cho DH. (2006). Changes in total polyphenol, total flavonoid contents and antioxidant activities of Hibiscus cannabinus L. Korean Journal of Medicinal Crop Science. 14:307-310.
  27. Santos AP, Segura-Muoz SI, Nadal M, Schuhmacher M, Domingo JL, Martinez CA and Magosso Takayanagui AM. (2015). Traffic-related air pollution biomonitoring with Tradescantia pallida(Rose) Hunt. cv. purpurea Boom in Brazil. Environmental Monitoring and Assessment. 187:39. https://doi.org/10.1007/s10661-014-4234-3
  28. Sharma OP and Bhat TK. (2009). DPPH antioxidant assay revisited. Food Chemistry. 113:1202-1205. https://doi.org/10.1016/j.foodchem.2008.08.008
  29. Shi ZU, Lin MI and Francis FJ. (1992). Anthocyanins of Tradescantia pallida. potential food colorants. Journal of Food Science. 57:761-765. https://doi.org/10.1111/j.1365-2621.1992.tb08090.x
  30. Shon HK, Lee YS, Park YH, Kim MJ and Lee KA. (2008). Physico-chemical properties of Gugija(Lycii fructus) extracts. Korean Journal of Food Cookery Science. 24:905-911.
  31. Tadera K, Minami Y, Takamatsu K and Matsuoka T. (2006). Inhibition of ${\alpha}$-glucosidase and ${\alpha}$-amylase by flavonoids. Journal of Nutritional Science and Vitaminology. 52:149-153. https://doi.org/10.3177/jnsv.52.149
  32. Tan JBL, Yap WJ, Tan SY, Lim YY and Lee SM. (2014). Antioxidant content, antioxidant activity, and antibacterial activity of five plants from the Commelinaceae family. Antioxidants. 3:758-769. https://doi.org/10.3390/antiox3040758
  33. Thewes MR, Junior DE and Droste A. (2011). Genotoxicity biomonitoring of sewage in two municipal wastewater treatment plants using the Tradescantia pallida var. purpurea bioassay. Genetics and Molecular Biology. 34:689-693. https://doi.org/10.1590/S1415-47572011005000055
  34. Toeller M. (1994). ${\alpha}$-Glucosidase inhibitors in diabetes: Efficacy in NIDDM subjects. European Journal of Clinical Investigation. 24:31-35.
  35. Urquiaga I and Leighton F. (2000). Plant polyphenol antioxidants and oxidative stress. Biological Research. 33:55-64.
  36. Yang DS, Pennisi SV, Son KC and Kays SJ. (2009). Screening indoor plants for volatile organic pollutant removal efficiency. HortScience. 44:1377-1381.

Cited by

  1. var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract vol.43, pp.2, 2010, https://doi.org/10.4163/kjn.2010.43.2.105