Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography-electron spray ionization-MS/MS

  • Sun, Ting-Ting (School of Pharmacy, Shenyang Pharmaceutical University) ;
  • Liang, Xin-Lei (School of Pharmacy, Shenyang Pharmaceutical University) ;
  • Zhu, He-Yun (School of Pharmacy, Shenyang Pharmaceutical University) ;
  • Peng, Xu-Ling (School of Pharmacy, Shenyang Pharmaceutical University) ;
  • Guo, Xing-Jie (School of Pharmacy, Shenyang Pharmaceutical University) ;
  • Zhao, Long-Shan (School of Pharmacy, Shenyang Pharmaceutical University)
  • Received : 2015.04.07
  • Accepted : 2015.07.28
  • Published : 2016.07.15
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Abstract

Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLC-MS/MS) method was developed to separate and identify saponins in Shizhu ginseng. Results: The separation was carried out on a Waters ACQUITY UPLC BEH $C_{18}$ column ($100mm{\times}2.1mm$, $1.7{\mu}m$) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at $40^{\circ}C$. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances. Conclusion: The developed UHPLC-MS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.

Keywords

References

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