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Effect of Korean Red Ginseng extraction conditions on antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content: optimization using response surface methodology

  • Received : 2015.06.16
  • Accepted : 2015.08.05
  • Published : 2016.07.15

Abstract

Background: Extraction conditions greatly affect composition, as well as biological activity. Therefore, optimization is essential for maximum efficacy. Methods: Korean Red Ginseng (KRG) was extracted under different conditions and antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content evaluated. Optimized extraction conditions were suggested using response surface methodology for maximum antioxidant activity and extraction yield. Results: Analysis of KRG extraction conditions using response surface methodology showed a good fit of experimental data as demonstrated by regression analysis. Among extraction factors, such as extraction solvent and extraction time and temperature, ethanol concentration greatly affected antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content. The optimal conditions for maximum antioxidant activity and extraction yield were an ethanol concentration of 48.8%, an extraction time 73.3 min, and an extraction temperature of $90^{\circ}C$. The antioxidant activity and extraction yield under optimal conditions were 43.7% and 23.2% of dried KRG, respectively. Conclusion: Ethanol concentration is an important extraction factor for KRG antioxidant activity and extraction yield. Optimized extraction conditions provide useful economic advantages in KRG development for functional products.

Acknowledgement

Supported by : Korea Small and Medium Business Administration

References

  1. Bae KH. The medicinal plants of Korea. 8th ed. Seoul: Kyo-Hak Publishing Co.; 2000.
  2. Im DS, Nah SY. Yin and yang of ginseng pharmacology: ginsenosides vs gintonin. Acta Pharmacol Sin 2013;34:1367-73. https://doi.org/10.1038/aps.2013.100
  3. Wong AS, Che CM, Leung KW. Recent advances in ginseng as cancer therapeutics: a functional and mechanistic overview. Nat Prod Rep 2015;32:256-72. https://doi.org/10.1039/C4NP00080C
  4. Jung JH, Kang IG, Kim DY, Hwang YJ, Kim ST. The effect of Korean red ginseng on allergic inflammation in a murine model of allergic rhinitis. J Ginseng Res 2013;37:167-75. https://doi.org/10.5142/jgr.2013.37.167
  5. Gonzalez-Burgos E, Fernandez-Moriano C, Gomez-Serranillos MP. Potential neuroprotective activity of Ginseng in Parkinson's disease: a review. J Neuroimmune Pharmacol 2015;10:14-29. https://doi.org/10.1007/s11481-014-9569-6
  6. Kim K. Effect of ginseng and ginsenosides on melanogenesis and their mechanism of action. J Ginseng Res 2015;39:1-6. https://doi.org/10.1016/j.jgr.2014.10.006
  7. Nam KY. The comparative understanding between red ginseng and white ginsengs, processed ginsengs (Panax ginseng Meyer). J Ginseng Res 2005;29:1-18. https://doi.org/10.5142/JGR.2005.29.1.001
  8. Park JD, Lee YH, Kim SI. Ginsenoside Rf2, a new dammarane glycoside from Korean red ginseng (Panax ginseng). Arch Pharm Res 1998;21:615-7. https://doi.org/10.1007/BF02975384
  9. Hwang CR, Lee SH, Jang GY, Hwang IG, Kim HY, Woo KS, Lee J, Jeong HS. Changes in ginsenoside compositions and antioxidant activities of hydroponic-cultured ginseng roots and leaves with heating temperature. J Ginseng Res 2014;38:180-6. https://doi.org/10.1016/j.jgr.2014.02.002
  10. Yamabe N, Song KI, Lee W, Han IH, Lee JH, Ham J, Kim SN, Park JH, Kang KS. Chemical and free radical-scavenging activity changes of ginsenoside Re by maillard reaction and its possible use as a renoprotective agent. J Ginseng Res 2012;36:256-62. https://doi.org/10.5142/jgr.2012.36.2.256
  11. Kim MH, Lee YC, Choi SY, Cho CW, Rho J, Lee KW. The changes of ginsenoside patterns in red ginseng processed by organic acid impregnation pretreatment. J Ginseng Res 2011;35:497-503. https://doi.org/10.5142/jgr.2011.35.4.497
  12. Murthy HN, Dandin VS, Lee EJ, Paek KY. Efficacy of ginseng adventitious root extract on hyperglycemia in streptozotocin-induced diabetic rats. J Ethnopharmacol 2014;153:917-21. https://doi.org/10.1016/j.jep.2014.03.062
  13. Siddiqi MZ, Siddiqi MH, Kim YJ, Jin Y, Huq MA, Yang DC. Effect of fermented red ginseng extract enriched in ginsenoside Rg3 on the differentiation and mineralization of preosteoblastic MC3T3-E1 Cells. J Med Food 2015;18:542-8. https://doi.org/10.1089/jmf.2014.3251
  14. Lee S, Lee YH, Park JM, Bai DH, Jang JK, Park YS. Bioconversion of ginsenosides from red ginseng extract using candida allociferrii JNO301 isolated from Meju. Mycobiology 2014;42:368-75. https://doi.org/10.5941/MYCO.2014.42.4.368
  15. Zhang WM, Huang WY, Chen WX, Han L, Zhang HD. Optimization of extraction condition of areca seed polyphenols and evaluation of their antioxidant activities. Molecules 2014;19:16416-27. https://doi.org/10.3390/molecules191016416
  16. Gan C-Y, Latiff AA. Optimization of the solvent extraction of bioactive compounds from Parkia speciosa pod using response surface methodology. Food Chem 2011;124:1277-83. https://doi.org/10.1016/j.foodchem.2010.07.074
  17. Jeong JY, Jo YH, Kim SB, Liu Q, Lee JW, Mo EJ, Lee KY, Hwang BY, Lee MK. Pancreatic lipase inhibitory constituents from Morus alba leaves and optimization for extraction conditions. Bioorg Med Chem Lett 2015;25:2269-74. https://doi.org/10.1016/j.bmcl.2015.04.045
  18. Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta 2008;76:965-77. https://doi.org/10.1016/j.talanta.2008.05.019
  19. Ferreira SLC, Bruns RE, Ferreira HS, Matos GD, David JM, Brandao GC, Da Silva EGP, Portugal LA, Reis PS, Souza AS, et al. Box-Behnken design: an alternative for the optimization of analytical methods. Anal Chim Acta 2007;597:179-86. https://doi.org/10.1016/j.aca.2007.07.011
  20. Jeong JY, Jo YH, Lee KY, Do SG, Hwang BY, Lee MK. Optimization of pancreatic lipase inhibition by Cudrania tricuspidata fruits using response surface methodology. Bioorg Med Chem Lett 2014;24:2329-33. https://doi.org/10.1016/j.bmcl.2014.03.067
  21. Betteridge DJ. What is oxidative stress? Metabolism 2000;49:3-8.
  22. Yoshikawa T, Naito Y. What is oxidative stress? J Japan Med Ass 2002;45:271-6.
  23. Gostner JM, Becker K, Ueberall F, Fuchs D. The good and bad of antioxidant foods: an immunological perspective. Food Chem Toxicol 2015;80:72-9. https://doi.org/10.1016/j.fct.2015.02.012
  24. Garcia-Nino WR, Zazueta C. Ellagic acid: pharmacological activities and molecular mechanisms involved in liver protection. Pharmacol Res 2015;97:84-103. https://doi.org/10.1016/j.phrs.2015.04.008
  25. de Oliveira CC, Araujo Calado VM, Ares G, Granato D. Statistical approaches to assess the association between phenolic compounds and the in vitro antioxidant activity of Camellia sinensis and Ilex paraguariensis teas. Crit Rev Food Sci Nutr 2015;55:1456-73. https://doi.org/10.1080/10408398.2012.750233
  26. Sohn SH, Kim SK, Kim YO, Kim HD, Shin YS, Yang SO, Kim SY, Lee SW. A comparison of antioxidant activity of Korean white and red ginsengs on $H_{2}O_{2}$-induced oxidative stress in HepG2 hepatoma cells. J Ginseng Res 2013;37:442-50. https://doi.org/10.5142/jgr.2013.37.442
  27. Lim KH, Cho JY, Kim B, Bae BS, Kim JH. Red ginseng (Panax ginseng) decreases isoproterenol-induced cardiac injury via antioxidant properties in porcine. J Med Food 2014;17:111-8. https://doi.org/10.1089/jmf.2013.2768
  28. Seo SK, Hong Y, Yun BH, Chon SJ, Jung YS, Park JH, Cho S, Choi YS, Lee BS. Antioxidative effects of Korean red ginseng in postmenopausal women: a double-blind randomized controlled trial. J Ethnopharmacol 2014;154:753-7. https://doi.org/10.1016/j.jep.2014.04.051
  29. Pan HY, Qu Y, Zhang JK, Kang TG, Dou DQ. Antioxidant activity of ginseng cultivated under mountainous forest with different growing years. J Ginseng Res 2013;37:355-60. https://doi.org/10.5142/jgr.2013.37.355
  30. Jin Y, Kim YJ, Jeon JN, Wang C, Min JW, Noh HY, Yang DC. Effect of white, red, and black ginseng on physicochemical properties and ginsenosides. Plant Foods Hum Nutr 2015;70:141-5. https://doi.org/10.1007/s11130-015-0470-0
  31. Smith I, Williamson EM, Putnam S, Farrimond J, Whalley BJ. Effects and mechanisms of ginseng and ginsenosides on cognition. Nutr Rev 2014;72:319-33. https://doi.org/10.1111/nure.12099
  32. Yamabe N, Kim YJ, Lee S, Cho EJ, Park SH, Ham J, Kim HY, Kang KS. Increase in antioxidant and anticancer effects of ginsenoside Re-lysine mixture by Maillard reaction. Food Chem 2013;138:876-83. https://doi.org/10.1016/j.foodchem.2012.12.004
  33. Jiang Z, Wang Y, Zhang X, Peng T, Li Y, Zhang Y. Protective effect of ginsenoside R0 on anoxic and oxidative damage in vitro. Biomol Ther (Seoul) 2012;20:544-9. https://doi.org/10.4062/biomolther.2012.20.6.544
  34. Wang Y, Dong J, Liu P, Lau CW, Gao Z, Zhou D, Tang J, Ng CF, Huang Y. Ginsenoside Rb3 attenuates oxidative stress and preserves endothelial function in renal arteries from hypertensive rats. Br J Pharmacol 2014;171:3171-81. https://doi.org/10.1111/bph.12660
  35. Lee LS, Cho CW, Hong HD, Lee YC, Choi UK, Kim YC. Hypolipidemic and antioxidant properties of phenolic compound-rich extracts from white ginseng (Panax ginseng) in cholesterol-fed rabbits. Molecules 2013;18:12548-60. https://doi.org/10.3390/molecules181012548
  36. Han Y, Xu Q, Hu JN, Han XY, Li W, Zhao LC. Maltol, a food flavoring agent, attenuates acute alcohol-induced oxidative damage in mice. Nutrients 2015;7:682-96. https://doi.org/10.3390/nu7010682
  37. Jiao L, Li B, Wang M, Liu Z, Zhang X, Liu S. Antioxidant activities of the oligosaccharides from the roots, flowers and leaves of Panax ginseng C.A. Meyer. Carbohydr Polym 2014;106:293-8. https://doi.org/10.1016/j.carbpol.2014.02.035
  38. Wang J, Sun C, Zheng Y, Pan H, Zhou Y, Fan Y. The effective mechanism of the polysaccharides from Panax ginseng on chronic fatigue syndrome. Arch Pharm Res 2014;37:530-8. https://doi.org/10.1007/s12272-013-0235-y