Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Total ginsenosides suppress monocrotaline-induced pulmonary hypertension in rats: involvement of nitric oxide and mitogen-activated protein kinase pathways

  • Qin, Na (Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine) ;
  • Yang, Wei (Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine) ;
  • Feng, Dongxu (Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine) ;
  • Wang, Xinwen (Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine) ;
  • Qi, Muyao (Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine) ;
  • Du, Tianxin (Department of Pharmacy, Luoyang Orthopedic Hospital, Orthopedics Hospital of Henan Province) ;
  • Sun, Hongzhi (Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University School of Medicine) ;
  • Wu, Shufang (Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine)
  • Received : 2015.06.18
  • Accepted : 2015.09.30
  • Published : 2016.07.15
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Abstract

Background: Ginsenosides have been shown to exert beneficial pharmacological effects on the central nervous, cardiovascular, and endocrine systems. We sought to determine whether total ginsenosides (TG) inhibit monocrotaline (MCT)-induced pulmonary hypertension and to elucidate the underlying mechanism. Methods: MCT-intoxicated rats were treated with gradient doses of TG, with or without $N^G$-nitro-$\small{L}$-arginine methyl ester. The levels of molecules involving the regulation of nitric oxide and mitogen-activated protein kinase pathways were determined. Results: TG ameliorated MCT-induced pulmonary hypertension in a dose-dependent manner, as assessed by the right ventricular systolic pressure, the right ventricular hypertrophy index, and pulmonary arterial remodeling. Furthermore, TG increased the levels of pulmonary nitric oxide, endothelial nitric oxide synthase, and cyclic guanosine monophosphate. Lastly, TG increased mitogen-activated protein kinase phosphatase-1 expression and promoted the dephosphorylation of extracellular signal-regulated protein kinases 1/2, p38 mitogen-activated protein kinase, and c-Jun NH2-terminal kinase 1/2. Conclusion: TG attenuates MCT-induced pulmonary hypertension, which may involve in part the regulation of nitric oxide and mitogen-activated protein kinase pathways.

Keywords

References

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