DOI QR코드

DOI QR Code

Antioxidant Activities and Anti-aging Effects of Orostachys japonicus A. Berger Extracts

와송 추출물의 항산화 활성 및 항노화 효과

  • Jung, Da-Jung (Department of Microbial Engineering, Konkuk University) ;
  • Choe, Tae-Boo (Department of Microbial Engineering, Konkuk University)
  • 정다정 (건국대학교 일반대학원 생물공학과) ;
  • 최태부 (건국대학교 일반대학원 생물공학과)
  • Received : 2016.05.13
  • Accepted : 2016.06.23
  • Published : 2016.06.30

Abstract

This study tested the antioxidant activities and anti-aging effects of an extract of Orostachys japonicus, a herb used as a folk remedy for cancer for a long time. To assess the antioxidant activities of the Orostachys japonicus extract (OJE), the total polyphenol content was confirmed to have a high caffeic acid equivalent weight of 12 g by dry weight of 100g OJE and the total flavonoid content was confirmed that the quercetin equivalent of 1.7 g by dry weight of 100g OJE. DPPH free radical scavenging activity was confirmed a high scavenging activity of 79.7% at a concentration of 1%. To confirm the anti-aging effect of OJE, MMP-1 expression was decreased in $25{\mu}L/mL$ and $50{\mu}L/mL$ similar to control. In a clinical experiment, a group of subjects who used a cosmetic product containing OJE showed a significant increase in the amount of skin moisture from the forehead, left cheek, and chin. The experimental group showed a significant increase in the amount of skin sebum from the chin and maintaining pH balance of normal skin, As a result, the OJE demonstrates the efficacy of natural cosmetic material as antioxidant and anti-aging.

본 연구는 오래전부터 민간요법으로 암의 치료제로 사용된 약초인 와송 추출물의 항산화 활성과 항노화 효능을 검증하였다. 와송 추출물의 항산화 활성을 측정한 결과, 총 폴리페놀 함량은 100 g의 와송 추출물 건조중량 당 12 g의 높은 caffeic acid 당량이 함유되어 있는 것을 확인하였고, 총 플라보노이드 함량은 100 g의 와송 추출물 건조중량 당 1.7 g의 quercetin 당량이 함유되어 있는 것을 확인하였다. DPPH free radical 소거능은 와송 추출물 1%의 농도에서 79.7%의 높은 소거능을 확인하였다. 또한 와송 추출물의 항노화 효능 측정 결과 $25{\mu}g/mL$, $50{\mu}g/mL$ 농도에서 control과 유사한 MMP-1 발현 억제능을 확인하였다. 와송 추출물의 임상실험결과 와송 추출물 함유 화장품 사용군의 수분함량은 오른쪽 볼과 코, 턱의 부위에서 통계적으로 유의하게 증가하였고, 정상적인 피부의 유분 함량과 pH를 유지하는 결과를 확인하였다. 이상의 결과에 따라 와송 추출물은 항산화와 항노화 효능을 가진 천연 화장품 소재로서의 활용 가능성이 있다고 사료된다.

Keywords

References

  1. G. M. Ahn, T. W. Kang, J. H. Jeong, B. K. Jo, Clinical studies on the antiirritation effects of Mung Bean (Phaseolus aureus) extract in cosmetics. J Soc Cosmet Scientists Kor 30(1), 23-28 (2004).
  2. M. H. Song, J. T. Bae, G. S. Lee, Y. H. Zhang, H. B. Pyo, A study on the Azolla imbricata using as a cosmetic active ingredient. J Soc Cosmet Scient 36(1), 71-77 (2010).
  3. S. E. Kim, S. R. Kang, M. S. Park, Y. C. Kim, Alleviating effects of Cnidium officinale water extract on skin barrier and inflammation in hairless mouse. J Invest Cosmetol 7(2), 137-145 (2011). https://doi.org/10.15810/jic.2011.7.2.007
  4. I. S. Bae, M. J. Shim, J. Y. Lee, Y. C. Kim, Application tests of Phellodendrin cortex water extract as functional cosmetic raw materials. J Invest Cosmetol 8(1), 1-8 (2012). https://doi.org/10.15810/jic.2012.8.1.001
  5. S. E. Kim, Y. C. Kim, Inhibitory effect of Cnidium officinale water extract on the skin wrinkle formation in hairless mouse. J Invest Cosmetol 9(3), 203-211 (2013). https://doi.org/10.15810/jic.2013.9.3.001
  6. S. J. Yang, T. B. Choe, Antioxidant Activity and Whitening Effect of Forsythiae Fructus Extracts. J Medicinal Crop Sci. 19(6), 472-477 (2011). https://doi.org/10.7783/KJMCS.2011.19.6.472
  7. G. N. Lim, M. A. Park, S. N. Park, Antioxidative and Antiaging Effects of Sorbus commixta Twig Extracts. J Medicinal Crop Sci. 28(4), 482-490 (2011).
  8. M. H. Kim, T. B. Choe, Anti-oxidant Activity of Akebia quinata fruit extract and the Effects of Skin. J Medicinal Crop Sci. 32(3), 439-450 (2015).
  9. D. A. Kim, Anti-microbial and antioxidative activities of extracts from herbal medicine for cosmetics. Master's thesis, Department of Chemistry and Engineering, Konkuk University, Seoul, Republic of Korea, pp. 1-83 (2010).
  10. H. J. Park, S. C. Lim, M. S. Lee, H. S. Young, Triterpene and steroids from Orostachys japonicus. Kor J Pharmacogn 25(1), 20-23 (1994)
  11. S. Y. Choi, J. G. Kim, N. J. Sung, Studies on the physicochemical characteristics and NDMA formation of Orostachys japonicus A. Berger. Korean J Food & Nutr 21(20, 148-156 (2008).
  12. S. J. Lee, J. K. Seo, J. H. Shin, H. J. Lee, N. J. Sung, Antioxidant activity of Wa-song (Orostachys japonicus A. Berger) according to drying methods. J Kor Soc Food Sci Nutr 37(5), 605-611 (2008). https://doi.org/10.3746/jkfn.2008.37.5.605
  13. S. Y. Yoon, S. Y. Lee, K. B. W. R. Kim, E. J. Song, S. J. Kim, S. J. Lee, C. H. Lee, D. H. Ahn, Antimicrobial activity of the solvent extract from different parts of Orostachys japonicus. J Kor Soc Food Sci Nutr 38(1), 14-18 (2009). https://doi.org/10.3746/jkfn.2009.38.1.014
  14. H. Kwon, K. S. Han, Effects of Orostachys japonicus A. Berger on the immune system. Kor J Medicinal Corp Sci 12(4), 315-320 (2004).
  15. H. S. Lee, D. S. Ryu, G. S. Lee, D. S. Lee, Anti-inflammatory effects of dichloromethane fraction from Orostachys japonicus in RAW 264.7 cells: suppression of NF-kB activation and MAPK signaling. J Ethnopharmacol 140(2), 271-276 (2012). https://doi.org/10.1016/j.jep.2012.01.016
  16. Y. S. Won, J. H. Lee, S. J. Kwon, D. U. Ahn, D. Y. Shin, K. I. Seo, Anticancer effects of cultivated Orostachys japonicus on human prostate cancer cells. J Kor Soc Food Sci Nutr 43(1), 67-73 (2014). https://doi.org/10.3746/jkfn.2014.43.1.067
  17. O. Folin, W. Denis, On phosphotungsticphosphomoiybdic compounds ascolorregents. J Biochem, 12(2), 239-249 (1912).
  18. T. Gutfinger, Polyphenols in olive oils. J American. Oil Chem Soc, 58(11), 966-968 (1981). https://doi.org/10.1007/BF02659771
  19. M. I. Moreno, M. I. Isla, A. R. Sampietro, M. A. Vattuone, Comparison of the free radical scavenging activity of propolis from several region of argentina. J Ethnophamacol, 71(1), 109-114 (2000). https://doi.org/10.1016/S0378-8741(99)00189-0
  20. M. S. Blois, Antioxidant determination by the use of a stable free radical. Nature, 181(4617), 1199-1200 (1958). https://doi.org/10.1038/1811199a0
  21. E. N. Frankel, J. B. German, J. E. Kinsella, E. Parks, J. Kanner, Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine, J Lancet, 341(8843), 454-457 (1993). https://doi.org/10.1016/0140-6736(93)90206-V
  22. C. A. Rice-Evans, N. J. Miller, G. Paganga, Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radic bio med, 20(7), 933-956 (1996). https://doi.org/10.1016/0891-5849(95)02227-9
  23. J. B. Harborne, C. A. Williams, Advances in flavonoid research since 1992, Phytochemistry. 55(6), 481-504 (2000). https://doi.org/10.1016/S0031-9422(00)00235-1
  24. R. J. Gryglewski, R. Korbut, J. Robak, J. Swies, On the mechanism of antithrombotic action of flavonoids. Biochem pharmacol, 36(3), 317-322 (1987). https://doi.org/10.1016/0006-2952(87)90288-7
  25. S. J. Lee, E. J. Song, S. Y. Lee, K. B. W. R. Kim, S. J. Kim, S. Y. Yoon, C. J. Lee, D. H. Ahn, Antioxidant Activity of Leaf, Stem and Root Extracts from Orostachys japonicus and Their Heat and pH Stabilities, J Korean Soc Food Sci Nutr, 38(11), 1571-1579 (2009). https://doi.org/10.3746/jkfn.2009.38.11.1571
  26. E. Y. Kim, I. H. Baik, J. H. Kim, S. R. Kim, M. R. Rhyu, Screening of the Antioxidant Activity of Some Medicinal Plants ,Kor J Food Sci. Technol, 36(2), 333-338 (2004).
  27. I. H. Baek, Antioxidant activity and skin improvement effects of Smilacis glabrae Rhizoma extracts. Major of Esthetics, Department of Beauty Art, The Graduate School of Seokyeong University (2012).
  28. S. K. Kim, Study on the antiatherosclerosis functional materization of natural antioxidant from the aerial part of Saururus chinensis Baill. Department of Food Science and Technology Graduate School, Kyungpook National University (2009).
  29. H. Y. Ahn, D. J. Choe, Y. S. Cho, Antioxidant Activity and Chemical Characteristics of Orostachys malacophyllus and Fermented. J Life Science, 25(5), 577-584 (2015). https://doi.org/10.5352/JLS.2015.25.5.577
  30. S. Y. Choi, Effect of Orostachys japonicus extracts on antioxidative activity and N-nitrosodimethylamine formation. Department of Food Science and Nutrition, College of Natural Science, Gyeongsang National University, Master's thesis (2006).
  31. Y. H. Jeon, M. H. Kim, M. R. Kim, Antioxidative, Antimutagenic, and Cytotoxic Activities of Ethanol Extracts from Cornus officianalis. J Korean Soc Food Sci Nutr, 37(1), 1-7 (2008). https://doi.org/10.3746/jkfn.2008.37.1.1
  32. H. S. Kang, J. S. Kang, W. S. Jeong, Cytotoxic and Apoptotic Effects of Saponins from Akebia quinata on HepG2 Hepatocarcinoma Cells. Kor J. Food Preserv. 17(3), 311-319 (2010).
  33. S. K. Park, Studies on the proteome profiling for anti-aging efficacy of polypeptides from Astragalus membranaceus Bunge. Aju University, D. Thesis (2015).
  34. Y. A. Choi, Signaling pathway of MMP-2 in fibroblast migration and chondrogenic differentiation. Department of Biology, major in Zoology, The Graduate School (2007).
  35. Farwick M, Watson RE, Rawlings AV, Wollenweber U, Lersch P, Bowden JJ, Salicyloyl-phytosphingosine: a novel agent for the repair of photoaged skin. Int J. Cosmet Sci, 29(4): 319-329, 2007. https://doi.org/10.1111/j.1467-2494.2007.00394.x
  36. Maity N, Nema NK, Abedy MK, Sarkar BK, Mukherjee pK. Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity. J. Ethnopharmacol., 137(3): 1300-1305, 2011. https://doi.org/10.1016/j.jep.2011.07.064
  37. Y. K. Muramatsu, K. Shita, Water and skin and NMF. Frag J, 13, 518 (1993).
  38. R. Ghadially, B. E. Brown, S. M. Sequeira-Martin, K. R. Feingold, P. M. Elias, The aged epidermal permeability barrier: Structural, functional, and lipid biochemical abnormalities in humans and a senescent murine model. J Clinical Investigation, 95(5), 2281-2290 (1995). https://doi.org/10.1172/JCI117919
  39. R. Blanken, M. I. van Vilsteren, R. A. Tupker, P. J. Coenraads, Effect of mineral oil and linoleic-acid-containing emulsions on the skin vapour loss of sodium-laurylsulphate-induced irritant skin reactions. Contact dermatitis, 20(2), 93-97 (1989). https://doi.org/10.1111/j.1600-0536.1989.tb03114.x
  40. J. W. Fluhr, M. J. Behne, B. E. Brown, D. G. Moskowitz, C. Selden, M. Mao-Qiang, T. M. Mauro, P. M. Elias, K. R. Feingold, Stratum corneum acidification in neonatal skin: Secretory phospholipase A2 and the sodium/ hydrogen antiporter-1 acidify neonatal rat stratum corneum. J Invesitigative dermatology, 122(2), 320-329 (2004). https://doi.org/10.1046/j.0022-202X.2003.00204.x
  41. A. K. Han, J. S. Won, O. S. Kim, Skin Hydration Status and Skin Surface pH According to the Body Parts of the Aged with Immobilization. J Kor Acad Fundam Nurs, 17(3), 314-323 (2010).

Cited by

  1. Identification of Flavoanoids From Finger Citron and Evaluation on Their Antioxidative and Antiaging Activities vol.7, pp.None, 2020, https://doi.org/10.3389/fnut.2020.584900