Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidant Activities and Anti-aging Effects of Orostachys japonicus A. Berger Extracts

와송 추출물의 항산화 활성 및 항노화 효과

  • Jung, Da-Jung (Department of Microbial Engineering, Konkuk University) ;
  • Choe, Tae-Boo (Department of Microbial Engineering, Konkuk University)
  • 정다정 (건국대학교 일반대학원 생물공학과) ;
  • 최태부 (건국대학교 일반대학원 생물공학과)
  • Received : 2016.05.13
  • Accepted : 2016.06.23
  • Published : 2016.06.30
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Abstract

This study tested the antioxidant activities and anti-aging effects of an extract of Orostachys japonicus, a herb used as a folk remedy for cancer for a long time. To assess the antioxidant activities of the Orostachys japonicus extract (OJE), the total polyphenol content was confirmed to have a high caffeic acid equivalent weight of 12 g by dry weight of 100g OJE and the total flavonoid content was confirmed that the quercetin equivalent of 1.7 g by dry weight of 100g OJE. DPPH free radical scavenging activity was confirmed a high scavenging activity of 79.7% at a concentration of 1%. To confirm the anti-aging effect of OJE, MMP-1 expression was decreased in $25{\mu}L/mL$ and $50{\mu}L/mL$ similar to control. In a clinical experiment, a group of subjects who used a cosmetic product containing OJE showed a significant increase in the amount of skin moisture from the forehead, left cheek, and chin. The experimental group showed a significant increase in the amount of skin sebum from the chin and maintaining pH balance of normal skin, As a result, the OJE demonstrates the efficacy of natural cosmetic material as antioxidant and anti-aging.

본 연구는 오래전부터 민간요법으로 암의 치료제로 사용된 약초인 와송 추출물의 항산화 활성과 항노화 효능을 검증하였다. 와송 추출물의 항산화 활성을 측정한 결과, 총 폴리페놀 함량은 100 g의 와송 추출물 건조중량 당 12 g의 높은 caffeic acid 당량이 함유되어 있는 것을 확인하였고, 총 플라보노이드 함량은 100 g의 와송 추출물 건조중량 당 1.7 g의 quercetin 당량이 함유되어 있는 것을 확인하였다. DPPH free radical 소거능은 와송 추출물 1%의 농도에서 79.7%의 높은 소거능을 확인하였다. 또한 와송 추출물의 항노화 효능 측정 결과 $25{\mu}g/mL$, $50{\mu}g/mL$ 농도에서 control과 유사한 MMP-1 발현 억제능을 확인하였다. 와송 추출물의 임상실험결과 와송 추출물 함유 화장품 사용군의 수분함량은 오른쪽 볼과 코, 턱의 부위에서 통계적으로 유의하게 증가하였고, 정상적인 피부의 유분 함량과 pH를 유지하는 결과를 확인하였다. 이상의 결과에 따라 와송 추출물은 항산화와 항노화 효능을 가진 천연 화장품 소재로서의 활용 가능성이 있다고 사료된다.

Keywords

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