Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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In Vitro Screening for Anti-Dementia Activities of Seaweed Extracts

해조류 추출물의 In Vitro 항치매 활성

  • Son, Hyun Jung (Division of Functional Food Research, Korea Food Research Institute) ;
  • Um, Min Young (Division of Functional Food Research, Korea Food Research Institute) ;
  • Kim, Inho (Division of Functional Food Research, Korea Food Research Institute) ;
  • Cho, Suengmok (Division of Functional Food Research, Korea Food Research Institute) ;
  • Han, Daeseok (Division of Functional Food Research, Korea Food Research Institute) ;
  • Lee, Changho (Division of Functional Food Research, Korea Food Research Institute)
  • 손현정 (한국식품연구원 기능성식품연구본부) ;
  • 엄민영 (한국식품연구원 기능성식품연구본부) ;
  • 김인호 (한국식품연구원 기능성식품연구본부) ;
  • 조승목 (한국식품연구원 기능성식품연구본부) ;
  • 한대석 (한국식품연구원 기능성식품연구본부) ;
  • 이창호 (한국식품연구원 기능성식품연구본부)
  • Received : 2016.03.25
  • Accepted : 2016.05.23
  • Published : 2016.07.31
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Abstract

We investigated that methanolic extracts of 20 kinds of seaweeds from Jeju Island for their antioxidant activities, acetylcholinesterase and ${\beta}$-secretase inhibitory activities, and neuronal survival in order to evaluate their potentials as anti-dementia agents. Ecklonia cava extracts had the highest total polyphenol content among the 20 seaweed extracts. The antioxidant activity of seaweed extracts was measured by using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. It was found that Ecklonia kurome extracts had the highest ABTS scavenging activity ($IC_{50}=0.07{\pm}0.01mg/mL$). As a result, Ecklonia cava, Ecklonia kurome, and Myelophycus simplex extracts were found to be the most effective in terms of acetylcholinesterase inhibitory activity. In the ${\beta}$-secretase activity assay, Ecklonia cava and Ecklonia kurome extracts were effectively inhibited ($84.41{\pm}1.70%$ and $81.17{\pm}2.43%$, respectively). As expected, neuronal cell death induced by $H_2O_2$ in SH-SY5Y cells was diminished by Ecklonia cava, Ecklonia kurome, and Sargassum yezoense extracts. Taken together, these results showed that Ecklonia cava extract has potential anti-dementia activity, which suggests that it might provide an effective strategy for improving dementia.

본 연구에서는 해조류 추출물 20종의 치매 예방 및 개선 소재로서의 가치를 검토하기 위해 총폴리페놀 함량, ABTS radical 소거능, acetylcholinesterase(AChE) 저해 활성, ${\beta}$-secretase 효소 억제 활성 및 신경세포 보호 효과를 비교하였다. 해조류 추출물의 총폴리페놀 함량과 ABTS radical 소거능을 측정한 결과 감태 추출물의 총폴리페놀 함량이 가장 높았으며, 검둥감태와 감태 추출물이 높은 ABTS radical 소거능을 나타내었다. AChE 저해 활성을 검토한 결과 감태, 검둥감태, 바위수염이 30% 이상의 높은 저해 활성을 보였고 그중 감태가 가장 높은 억제율을 나타내었다. 또한, ${\beta}$-secretase 효소 억제 활성은 검둥감태, 감태, 왜모자반 추출물에서 관찰되었다. $H_2O_2$에 의해 유도된 신경세포 독성에 대한 보호 효과는 감태, 검둥감태, 왜모자반 추출물 $100{\mu}g/mL$ 농도에서 관찰되었다. 이상의 결과로 미루어 보았을 때 감태 추출물이 치매 예방 및 개선제로서의 활용 가능성이 가장 뛰어난 것으로 생각되며, 추가로 생리활성 물질 구명 및 작용기전 입증을 위한 후속 연구가 진행되어야 할 것으로 생각한다.

Keywords

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