Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Changes in Platycoside Components and Antimicrobial Activities of Bronchus Disease-Inducing Bacteria of Fermented Platycodon grandiflorum Root by Lactic Acid Bacteria

젖산발효 처리에 의한 도라지의 Platycosides 조성 및 호흡기질환 유발세균에 대한 항균 활성 변화

  • Lee, Ka Soon (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Seong, Bong Jae (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Kim, Sun Ick (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Jee, Moo Geun (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Park, Saet Byeol (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Park, Myeong Hee (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Park, Shin Young (Fermented Food Science Division, National Institute of Agricultural Science, RDA) ;
  • Kim, Hyun Ho (Ginseng & Medicinal Plant Research Institute, CNARES)
  • 이가순 (충남농업기술원 인삼약초연구소) ;
  • 성봉재 (충남농업기술원 인삼약초연구소) ;
  • 김선익 (충남농업기술원 인삼약초연구소) ;
  • 지무근 (충남농업기술원 인삼약초연구소) ;
  • 박샛별 (충남농업기술원 인삼약초연구소) ;
  • 박명희 (충남농업기술원 인삼약초연구소) ;
  • 박신영 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 김현호 (충남농업기술원 인삼약초연구소)
  • Received : 2016.04.01
  • Accepted : 2016.06.21
  • Published : 2016.07.31
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Abstract

This study was performed in order to investigate changes in platycosides, as well as antimicrobial activities of bronchus diseases-inducing bacteria (Corynebacterium diphtheriae, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus pyogenes) of Platycodon grandiflorum root (PGR) fermented by lactic acid bacteria (Leuconostoc mesenteroides N12-4, Leuc. mesenteroides N58-5, Lactobacillus plantarum N76-10, L. plantarum N56-12, Lactobacillus brevis N70-9, and L. brevis E3-8). Growth of L. plantarum on PGR was most active during lactic acid fermentation using different strains. Total platycoside, platycoside E, platycodin A, polygalacin $D_2$, polygalacin D, and diapioplatyco-side E contents of PGR fermented for 96 h at $37^{\circ}C$ by Leuc. mesenteroides and L. plantarum increased, whereas contents of platycodin D and platycodin $D_3$ were reduced. The antimicrobial activity on PGR fermented by L. plantarum N56-12 exhibited strong microbial proliferation for all four kinds of bronchus disease-inducing bacteria and was higher than that of non-fermented PGR extract. MIC of fermented PGR extract by L. plantarum N56-12 on C. diphtheriae, K. pneumoniae, S. aureus, and S. pyogenes were 45, 10, 50, and 25 mg/mL, respectively. Thus, this result shows that the antimicrobial activities of bronchus disease-inducing bacteria and platycoside content of PGR by L. plantarum N56-12 were higher than that of non-fermented PGR extract.

기관지질환에 우수한 기능성을 가지고 있는 도라지는 수십종의 다양한 사포닌 종류를 함유하고 있다. 이러한 도라지를 젖산발효 시킬 경우 사포닌 조성의 변화와 기관지질환 유발 세균에 대한 항균 활성의 변화를 측정하였다. 도라지 발효를 위하여 사용된 젖산균은 Leuconostoc mesenteroides N12-4, Leuconostoc mesenteroides N58-5, Lactobacillus plantarum N76-10, Lactobacillus plantarum N56-12, Lactobacillus brevis N70-9 및 Lactobacillus brevis E3-8 등 6종이었다. 도라지 발효 시 젖산균 생육은 L. plantarum균이 가장 활발하였으며 pH 값도 가장 많이 내려가 발효 24시간째 pH는 3.73을 보였고 colony 수는 $10^7CFU/g$ 이상으로 증가하였다. 젖산발효 시간 동안 도라지 사포닌 9종에 대한 조성 변화는 사용된 도라지 시료에서 함량이 높았던 platycoside E, diapioplatycoside E, platycodin A, polygalacin D 등이 발효기간 중 일정량 감소하다가 발효 72시간 이후부터는 증가하는 경향이었고 platycodin D는 약간 증가하다가 72시간 이후부터는 오히려 감소하는 경향이었으며, 총사포닌 함량은 발효기간 동안 함량의 변화가 없었던 L. brevis E3-8균을 제외하고는 발효 72시간 이후 96시간째 증가하였다. 기관지질환 유발세균에 대한 항균 활성의 변화를 보기 위하여 사용된 세균은 Corynebacterium diphtheriae KCTC 3075, Klebsiella pneumoniae KCTC 2246, Staphylococcus aureus KCTC 1621 및 Streptococcus pyogenes ATCC 19615균 등 4종으로 각 균에 대한 항균 활성이 발효에 의하여 증대되는 젖산균은 L. plantarum균으로, 특히 L. plantarum N56-12균이 강한 항균 활성을 보여주었다. 이에 따라 각 기관지질환 유발세균에 대한 MIC를 조사한 결과 각 균에 대하여 도라지 무발효물은 200, 180, 210 및 90 mg/mL였고, L. plantarum N56-12균으로 발효된 도라지 상등액은 45, 10, 50 그리고 25 mg/mL였다.

Keywords

References

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