Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Anti-Oxidative and Anti-Inflammatory Effects of Cheongajihwang-Tang Extract on RAW264.7 Cells

청아지황탕(靑蛾地黃湯)의 RAW264.7 Cell에서 항산화 및 항염증 효능 연구

  • Yoon, Jeong-won (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Se-Myung University) ;
  • Kim, Soon-Joong (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Se-Myung University) ;
  • Park, Dong-Su (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Se-Myung University)
  • 윤정원 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 김순중 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 박동수 (세명대학교 한의과대학 한방재활의학과교실)
  • Received : 2016.06.23
  • Accepted : 2016.07.06
  • Published : 2016.07.31
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Abstract

Objectives This study was designed to investigate whether the Cheongajihwang-Tang (CT) has an inhibitory effect association with oxidation or inflammation in RAW264.7 cells. Methods Cytotoxic activity of CT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Nitric oxide production was measured using Griess reagent system. The total phenolic contents and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to evaluate the anti-oxidative effects of CT. Dichlorofluorescin diacetate (DCFH-DA) has been used as a substrate for measuring intracellular oxidant production. Results Cheongajihwang-Tang does not impair the cell viability in tested concentration. CT showed anti-oxidative effects in vitro by decreasing electron donating ability, and also showed anti-inflammatory effects suppressing NO and ROS expression in LPS induced RAW264.7 activation. CT inhibited the generation of intracellular ROS production as dose dependant manner. Conclusions CT has anti-oxidative effects and anti-inflammatory activities. These results indicate that CT extract has an anti-inflammatory activities via anti-oxidative effects.

Keywords

References

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