Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method

  • Jang, Sun Mi (Department of Conservative Dentistry, Yonsei University College of Dentistry) ;
  • Cho, Sin-Yeon (Department of Dentistry, Catholic Kwandong University International St. Mary's Hospital) ;
  • Kim, Eui-Seong (Department of Conservative Dentistry, Yonsei University College of Dentistry) ;
  • Jung, Il-Young (Department of Conservative Dentistry, Yonsei University College of Dentistry) ;
  • Lee, Seung Jong (Department of Conservative Dentistry, Yonsei University College of Dentistry)
  • Received : 2016.04.04
  • Accepted : 2016.06.20
  • Published : 2016.06.30
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Abstract

Purpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under $0^{\circ}C/2$ MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman's solution under $0^{\circ}C/2$ MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 34, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups. Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at $0^{\circ}C/2$ MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.

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