한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제45권8호
  • /
  • Pages.1162-1169
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  • 2016
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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DOI QR코드

DOI QR Code

반응표면분석법을 사용한 가속 용매 추출에 의한 홍삼 지표성분의 추출 최적화

Optimization of Extraction of Marker Compounds from Red Ginsengs by Accelerated Solvent Extraction Using Response Surface Methodology

  • 김은옥 (한국과학기술연구원 강릉분원 천연물융합연구센터) ;
  • 서구량 (한국과학기술연구원 강릉분원 천연물융합연구센터) ;
  • 엄병헌 (한국과학기술연구원 강릉분원 천연물융합연구센터)
  • Kim, Eun Ok (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Xu, Jiu Liang (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Um, Byung Hun (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
  • 투고 : 2016.04.05
  • 심사 : 2016.06.13
  • 발행 : 2016.08.31
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초록

본 연구에서는 기능성 홍삼 엑기스 제조를 위해 추출용매, 압력, 온도, 추출시간을 조절하여 가속 용매 추출한 후 기능성 홍삼제품의 지표성분인 진세노사이드 함량, 총페놀화합물 및 벤조피렌 함량 등을 조사하였다. 홍삼 가속 용매 추출 최적화를 위한 독립변수 선택을 위해 에탄올 농도, 압력, 온도 및 추출시간에 따른 기능성 홍삼제품의 지표성분인 진세노사이드 Rb1, Rg1 및 Rg3(20S) 함량의 합을 분석하여 진세노사이드 추출에 가장 최적의 용매를 50% 에탄올로 하고, 영향이 다소 작았던 요인인 압력을 2,500 psi로 설정하였으며, 온도와 추출시간을 독립변수로 설정하였다. 추출 온도 및 시간을 독립변수로 하여 고온고압 처리한 홍삼의 진세노사이드 Rb1, Rg1 및 Rg3(20S) 함량의 합, 총 페놀성 화합물 및 벤조피렌 함량을 측정한 결과, 벤조피렌이 검출되지 않는 조건에서 진세노사이드 Rb1, Rg1 및 Rg3(20S)의 함량 합이 최적화된 조건은 $158^{\circ}C$, 20분임을 확인할 수 있었다. 최적화된 조건에서의 가속 용매 추출 시 초음파 및 환류냉각 추출에 비해 적은 용매로 단시간 추출하면서 진세노사이드 Rb1, Rg1 및 Rg3(20S) 함량 합 및 총폴리페놀 함량이 각각 1.5~2배 및 2.2~5배 높게 추출할 수 있어 효율적인 추출방법이 될 것으로 생각된다.

A new method based on accelerated solvent extraction (ASE) combined with response surface methodology (RSM) has been developed for optimization of the extraction of ginsenoside [Rb1, Rg1, and Rg3(20S)], total phenolics, and benzopyrene in red ginseng. The RSM method, based on a five level and two variable central composite design, was employed to obtain the optimal combination of extraction conditions. In brief, ginsenosides Rb1, Rg1, and Rg3(20S) and total phenolics with undetectable benzopyrene were optimally extracted with 50% ethanol as an extraction solvent, extraction temperature of $158^{\circ}C$, extraction time of 20 min, extraction pressure of 2,500 psi, flush volume of 60%, and one extraction cycle. The contents of ginsenosides and total phenolics in red ginseng extracted by ASE under optimum conditions were significantly higher than those extracted by sonication and reflux extraction.

키워드

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  2. Antioxidant Effects of Raw Ginseng, Soft Red Ginseng, and Red Ginseng Sap vol.30, pp.9, 2016, https://doi.org/10.5352/jls.2020.30.9.763