Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-inflammatory effect of zaluzanin C on lipopolysaccharide-stimulated murine macrophages

지방질다당류로 자극한 마우스 대식세포에 있어서 zaluzanin C의 항염증 효과

  • Kang, Ye Rim (Department of Food and Nutrition, College of Engineering, Daegu University) ;
  • Lee, Hee Won (Department of Food and Nutrition, College of Engineering, Daegu University) ;
  • Kim, Yoon Hee (Department of Food and Nutrition, College of Engineering, Daegu University)
  • 강예림 (대구대학교 식품영양학과) ;
  • 이희원 (대구대학교 식품영양학과) ;
  • 김윤희 (대구대학교 식품영양학과)
  • Received : 2016.04.27
  • Accepted : 2016.06.10
  • Published : 2016.08.31
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Abstract

Zaluzanin C is a sesquiterpene lactone isolated from Ainsliaea acerifolia, a Korean medicinal plant. In the present study, the anti-inflammatory effects of zaluzanin C were demonstrated in lipopolysaccharide (LPS)-stimulated murine macrophages (RAW264.7 cells). Zaluzanin C inhibited the release of nitric oxide (NO) by alleviating the protein expression of inducible NO synthase in LPS-treated RAW264.7 cells. Furthermore, it suppressed the release of interleukin-6 induced by LPS. Zaluzanin C was also found to block the translocation of the p65 subunit of nuclear factor-kB from the cytosol to the nucleus, which is one of the underlying mechanisms of the anti-inflammatory action of zaluzanin C. These data suggest the potential of zaluzanin C in the treatment of inflammatory diseases.

천연물 유래 단일 성분의 항염증 효과에 대한 잠재성을 평가하는 스크리닝의 일환으로 11종의 단일 물질을 대상으로 항염증 효과를 탐색한 결과, 단풍취에서 분리정제한 zaluzanin C의 산화질소(II) 생성 억제능이 뛰어난 것을 확인하였다. 따라서 본 연구에서는 단풍취에서 분리정제한 zaluzanin C가 지질다당류로 자극한 마우스 대식세포인 RAW264.7 세포에서 염증반응에 미치는 영향에 대해 평가하고, 관련 메커니즘에 대해 검토하였다. Zaluzanin C는 LPS 자극에 의해 유도된 iNOS 단백질 발현양을 감소시킴으로써 산화질소(II) 생성을 억제할 뿐만 아니라 IL-6와 같은 염증 유발 사이토카인의 분비를 억제하였다. 이러한 효과는 전사인자인 NF-kB의 세포질에서 핵으로의 이동을 억제함으로써 나타나는 것으로 판단된다. 이러한 결과로부터, zaluzanin C가 염증 반응을 저해하는 효과가 있는 것으로 나타나, 향후 염증성 질환을 예방, 개선 및 치료하는데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다. 하지만 이를 위해서는 zaluzanin C의 생체 내 이용률 및 생리적 활성 농도 등에 대한 추가 연구가 필요한 것으로 생각된다.

Keywords

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