Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Isolation of Antifungal Compound and Biocontrol Potential of Lysobacter antibioticus HS124 against Fusarium Crown Rot of Wheat

  • Monkhung, Sararat (Crop Production Technology Program, Faculty of Animal Science and Agricultural Technology, Silpakorn University, Phetchaburi Information Technology Campus) ;
  • Kim, Yun-Tae (Future Agricultural Strategy Institute) ;
  • Lee, Yong-Seong (Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Cho, Jeong-Yong (Department of Food Engineering, Mokpo National University) ;
  • Moon, Jae-Hak (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Kim, Kil-Yong (Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • Received : 2016.08.02
  • Accepted : 2016.08.29
  • Published : 2016.08.31
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Abstract

Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed destructive alterations in the hyphal morphology of F. graminearum and almost degraded with $1,000{\mu}g\;mL^{-1}$ concentration. For further study, the antifungal compound extracted from the n-butanol crude extract of L. antibioticus HS124 was identified as N-Butyl-tetrahydro-5-oxofuran-2-carboxamide ($C_9H_{16}NO_3$) using NMR ($^1H-NMR$, $^{13}C-NMR$, $^1H-^1H\;COSY$, HMBC, and HMQC), and HR-ESI-MS analysis. To our knowledge, N-Butyl-tetrahydro-5-oxofuran-2-carboxamide may be a novel compound with molecular weight of 186.1130. The minimum inhibitory concentration value of antifungal compound was $62.5{\mu}g\;mL^{-1}$ against F. graminearum. In an in vivo pot experiment, crown rot disease from F. graminearum was inhibited when wheat seeds were treated with both HS124 culture and F. graminearum. Growth of wheat seedling was enhanced by treatment of HS124 compared to control. Our results suggest that L. antibioticus HS124 characterized in this study could be successfully used to control F. graminearum and could be used as an alternative to chemical fungicides in modern agriculture.

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References

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