The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Antibacterial Activity of Cinnamaldehyde and Estragole Extracted from Plant Essential Oils against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit

  • Song, Yu-Rim (Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University) ;
  • Choi, Min-Seon (Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University) ;
  • Choi, Geun-Won (Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University) ;
  • Park, Il-Kwon (Department of Forest Sciences, Seoul National University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
  • Received : 2016.01.04
  • Accepted : 2016.03.02
  • Published : 2016.08.01
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Abstract

Pseudomonas syringae pv. actinidiae (Psa) causes bacterial canker disease in kiwifruit. Antibacterial activity of plant essential oils (PEOs) originating from 49 plant species were tested against Psa by a vapor diffusion and a liquid culture assays. The five PEOs from Pimenta racemosa, P. dioica, Melaleuca linariifolia, M. cajuputii, and Cinnamomum cassia efficiently inhibited Psa growth by either assays. Among their major components, estragole, eugenol, and methyl eugenol showed significant antibacterial activity by only the liquid culture assay, while cinnamaldehyde exhibited antibacterial activity by both assays. The minimum inhibitory concentrations (MICs) of estragole and cinnamaldehyde by the liquid culture assay were 1,250 and 2,500 ppm, respectively. The MIC of cinnamaldehyde by the vapor diffusion assay was 5,000 ppm. Based on the formation of clear zones or the decrease of optical density caused by these compounds, they might kill the bacterial cells and this feature might be useful for managing the bacterial canker disease in kiwifruit.

Keywords

References

  1. Balestra, G. M., Mazzaglia, A., Quattrucci, A., Renzi, M. and Rossetti, A. 2009. Current status of bacterial canker spread on kiwifruit in Italy. Australas. Plant Dis. Notes 4:34-36.
  2. Catherine, A. A., Deepika, H. and Negi, P. S. 2012. Antibacterial activity of eugenol and peppermint oil in model food systems. J. Essent. Oil Res. 24:481-486. https://doi.org/10.1080/10412905.2012.703513
  3. Cooksey, D. A. 1994. Molecular mechanisms of copper resistance and accumulation in bacteria. FEMS Microbiol. Rev. 14:381-386. https://doi.org/10.1111/j.1574-6976.1994.tb00112.x
  4. Devi, K. P., Nisha, S. A., Sakthivel, R. and Pandian, S. K. 2010. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J. Ethnopharmacol. 130:107-115. https://doi.org/10.1016/j.jep.2010.04.025
  5. Di Lallo, G., Evangelisti, M., Mancuso, F., Ferrante, P., Marcelletti, S., Tinari, A., Superti, F., Migliore, L., D'Addabbo, P., Frezza, D., Scortichini, M. and Thaller, M. C. 2014. Isolation and partial characterization of bacteriophages infecting Pseudomonas syringae pv. actinidiae, causal agent of kiwifruit bacterial canker. J. Basic Microbiol. 54:1210-1221. https://doi.org/10.1002/jobm.201300951
  6. Di Pasqua, R., Hoskins, N., Betts, G. and Mauriello, G. 2006. Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol in the growing media. J. Agric. Food Chem. 54:2745-2749. https://doi.org/10.1021/jf052722l
  7. Ferguson, A. R. and Stanley, R. 2003. Kiwifruit. In: Encyclopedia of food sciences and nutrition, eds. by B. Caballero, L. C. Trugo and P. M. Finglas, pp. 3425-3431. Academic Press, Waltham, MA, USA.
  8. Garcia, C. V., Quek, S. Y., Stevenson, R. J. and Winz, R. A. 2012. Kiwifruit flavour: a review. Trends Food Sci. Technol. 24:82-91. https://doi.org/10.1016/j.tifs.2011.08.012
  9. Gill, A. O. and Holley, R. A. 2004. Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei. Appl. Environ. Microbiol. 70:5750-5755. https://doi.org/10.1128/AEM.70.10.5750-5755.2004
  10. Han, H. S., Nam, H. Y., Koh, Y. J., Hur, J. S. and Jung, J. S. 2003. Molecular bases of high-level streptomycin resistance in Pseudomonas marginalis and Pseudomonas syringae pv. actinidiae. J. Microbiol. 41:16-21.
  11. Iacobellis, N. S., Lo Cantore, P., Capasso, F. and Senatore, F. 2005. Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. J. Agric. Food Chem. 53:57-61. https://doi.org/10.1021/jf0487351
  12. Koh, Y. J., Kim, G. H., Jung, J. S., Lee, Y. S. and Hur, J. S. 2010. Outbreak of bacterial canker on Hort16A (Actinidia chinensis Planchon) caused by Pseudomonas syringae pv. actinidiae in Korea. N. Z . J. Crop Hortic. Sci. 38:275-282. https://doi.org/10.1080/01140671.2010.512624
  13. Koh, Y. J., Kim, G. H., Koh H. S., Lee, Y. S., Kim, S. C. and Jung, J. S. 2012. Occurrence of a new type of Pseudomonas syringae pv. actinidiae strain of bacterial canker on kiwifruit in Korea. Plant Pathol. J. 28:423-427. https://doi.org/10.5423/PPJ.NT.05.2012.0061
  14. Kotan, R., Cakir, A., Dadasoglu, F., Aydin, T., Cakmakci, R., Ozer, H., Kordali, S., Mete, E. and Dikbas, N. 2010. Antibacterial activities of essential oils and extracts of Turkish Achillea, Satureja and Thymus species against plant pathogenic bacteria. J. Sci. Food Agric. 90:145-160. https://doi.org/10.1002/jsfa.3799
  15. Lopez, P., Sanchez, C., Batlle, R. and Nerin, C. 2005. Solid-and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected foodborne bacterial and fungal strains. J. Agric. Food Chem. 53:6939-6946. https://doi.org/10.1021/jf050709v
  16. Mulyaningsih, S., Sporer, F., Zimmermann, S., Reichling, J. and Wink, M. 2010. Synergistic properties of the terpenoids aromadendrene and 1, 8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibioticresistant pathogens. Phytomedicine 17:1061-1066. https://doi.org/10.1016/j.phymed.2010.06.018
  17. Ooi, L. S., Li, Y., Kam, S. L., Wang, H., Wong, E. Y. and Ooi, V. E. 2006. Antimicrobial activities of cinnamon oil and cinnamaldehyde from the Chinese medicinal herb Cinnamomum cassia Blume. Am. J. Chin. Med. 34:511-522. https://doi.org/10.1142/S0192415X06004041
  18. Scortichini, M., Marcelletti, S., Ferrante, P., Petriccione, M. and Firrao, G. 2012. Pseudomonas syringae pv. actinidiae: a reemerging, multi-faceted, pandemic pathogen. Mol. Plant Pathol. 13:631-640. https://doi.org/10.1111/j.1364-3703.2012.00788.x
  19. Shahat, A. A., Ibrahim, A. Y., Hendawy, S. F., Omer, E. A., Hammouda, F. M., Abdel-Rahman, F. H. and Saleh, M. A. 2011. Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules 16:1366-1377. https://doi.org/10.3390/molecules16021366
  20. Song, Y. R., Park, I. K. and Oh, C. S. 2013. Two methods to test antibacterial activity of plant essential oils. Research Collection of Institute of Life Sciences & Resources, Kyung Hee University 32:61-65.
  21. Takikawa, Y., Serizawa, S., Ichikawa, T., Tsuyumu, S. and Goto, M. 1989. Pseudomonas syringae pv. actinidiae pv. nov.: the causal bacterium of canker of kiwifruit in Japan. Ann. Phytopathol. Soc. Jpn. 55:437-444. https://doi.org/10.3186/jjphytopath.55.437
  22. Wilson, C. L., Solar, J. M., El Ghaouth, A. and Wisniewski, M. E. 1997. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Dis. 81:204-210. https://doi.org/10.1094/PDIS.1997.81.2.204

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