Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Virucidal Efficacy against Avian Influenza Virus of a Disinfectant Spray Containing Grapefruit Seed Extracts, Citric Acid, Malic Acid and Benzalkonium Chloride

자몽종자추출물, 구연산, 사과산 그리고 염화벤잘코늄을 주성분으로 하는 스프레이형 소독제의 조류인플루엔자바이러스에 대한 살바이러스 효과

  • Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Yoo, Chang-Yeol (Department of Smart Information Convergence, Gyeongnam Provincial Namhae College) ;
  • Kim, Suk (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
  • 차춘남 (경상대학교 산업시스템공학과.공학연구원) ;
  • 박은기 (고신대학교 인문사회의학교실) ;
  • 정지윤 (공주대학교 특수동물학과) ;
  • 유창열 (경남도립남해대학 스마트융합정보학과) ;
  • 김석 (경상대학교 수의과대학.동물의학연구소) ;
  • 이후장 (경상대학교 수의과대학.동물의학연구소)
  • Received : 2016.04.18
  • Accepted : 2016.06.27
  • Published : 2016.08.31
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Abstract

Objectives: This study evaluated the virucidal efficacy against avian influenza virus (AIV) of a disinfectant spray containing 0.25% grapefruit seed extract, 0.2% citric acid, 0.0625% malic acid and 0.0125% benzalkonium chloride. Methods: The disinfectant spray was diluted several times with hard water (HW) and organic matter (OM). Two point five mL of each diluent was added into each test tube, and 2.5 mL of AIV suspension was inserted into each test tube. After 30 minutes of virus-disinfectant contact reaction at $4^{\circ}C$, 2.5 mL of 10% inactivated fetal bovine serum was added into each test tube to neutralize the sanitizer efficacy. The neutralized solutions were serial 10-fold dilutions with phosphate buffer solution, and 0.2 mL of the diluents was injected into the allantoic cavity of five ten-day-old-chickens per dilution time. After incubation of the embryos for five days, the viability of the AIV was examined by hemagglutination titer. The valid dilution of the disinfectant spray was estimated according to the dilution time that the virus titer was inactivated more than $10^4$ 50% egg-infective dose (EID50)/mL compared with pathogen control. Results: In HW and OM conditions, the valid dilutions of the disinfectant spray against AIV were seven- and three-fold dilutions, respectively. The AIV titer of the pathogen control was more than 6.1 log10EID50/mL, and there was no embryonic toxicity. Conclusion: The present study showed that this disinfectant spray has effective virucidal activity against AIV.

Keywords

References

  1. Kim YT. Current situation and elimination plan of influenza. J Korean Med Assoc. 2004; 47: 1116-1128. https://doi.org/10.5124/jkma.2004.47.11.1116
  2. World Health Organization. Cumulative number of confirmed human cases for avian influenza A(H5N1) reported to WHO, 2003-2016. Available: http://www.who.int/influenza/human_animal_interface/EN_GIP_20160404cumulativenumberH5N1-cases.pdf?ua=1 [accessed 18 April 2016].
  3. Si Y, Wang T, Skidmore AK, de Boer WF, Li L, Prins HHT. Environmental factors influencing the spread of the highly pathogenic avian influenza H5N1 virus in wild birds in Europe. Ecol Soc. 2010; 15: 26.
  4. Korea Animal Health Integrated System. Outbreak of legal communicable diseases: High pathogenic avian influenza. Available: http://www.kahis.go.kr/home/lkntscrinfo/selectLkntsOccrrncList.do?open-Flag=Y [accessed 18 April 2016].
  5. Woo BJ, Lee HW, Hwang YJ, Kim JN. Estimation of the economic loss by outbreak of highly pathogenic avian influenza, 1st ed. Naju: Korea Rural Economic Institute; 2008. p. 5-14.
  6. Swayne DE, Halvorson DA. Influenza. In: Saif YM, Barnes HJ, Fadly AM, Glisson JR, McDougald LR, Swayne DE. editors. Diseases of poultry, 11th ed. Iowa: Blackwell Science; 2003. p. 135-160.
  7. Fouchier RAM, Munster V, Wallensten A, Bestebroer TM, Herfest S, Smith D, et al. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. J Virol. 2005; 79: 2814-2822. https://doi.org/10.1128/JVI.79.5.2814-2822.2005
  8. Hitchner SB, Domermuth CH, Purchase HG, Williams JE. Isolation and identification of avian pathogens, 4th ed. College Station: American Association of Avian Pathologists; 1997. p. 140-145.
  9. Peiris JSM, De Jong MD, Guan Y. Avian influenza virus (H5N1): a threat to human health. Clin Micorbiol Rev. 2007; 20: 243-267. https://doi.org/10.1128/CMR.00037-06
  10. Cha CN, Lee YE, Kang IJ, Yoo CY, Park EK, An S, et al. Antiviral efficacy of Citra-kill$^{(R)}$, disinfectant solution against avian influenza virus. J Fd Hyg Safety. 2012; 27: 18-23. https://doi.org/10.13103/JFHS.2012.27.1.018
  11. Frier M. Derivatives of 4-Amino-quinaldinium and 8-Hydroxyquinoline. In: Hugo WB. editor. Inhibition and destruction of the microbial cell, 1st ed. London: Academic Press; 1971. p. 107-120.
  12. Sadakane K, Ichinose T. Effect of the hand antiseptic agents benzalkonium chloride, povidone-iodine, ethanol, and chlorhexidine gluconate on atopic dermatitis in NC/Nga mice. Int J Med Sci. 2015; 12: 116-125. https://doi.org/10.7150/ijms.10322
  13. Hohtola A. Bioactive compounds from northern plants. Adv Exp Med Biol. 2010; 698: 99-109. https://doi.org/10.1007/978-1-4419-7347-4_8
  14. Brandin H, Myrberg O, Rundlof T, Arvidsson AK, Brenning G. Adverse effects by artificial grapefruit seed extract products in patients on warfarin therapy. Eur J Clin Pharmacol. 2007; 63: 565-570. https://doi.org/10.1007/s00228-007-0289-1
  15. Shin WJ, Kim YK, Lee KH, Seong BL. Evaluation of the antiviral activity of a green tea solution as a hand-wash disinfectant. Biosci Biotechnol Biochem. 2012; 76: 581-584. https://doi.org/10.1271/bbb.110764
  16. Suarez DL, Spackman E, Senne DA, Bulaga L, Welsch AC, Froberg K. The effect of various disinfectants on detection of avian influenza virus by real time RT-PCR. Avian Dis. 2003; 47(3 Suppl): 1091-1095. https://doi.org/10.1637/0005-2086-47.s3.1091
  17. Hirneisen KA, Black EP, Cascarino JL, Fino VR, Hoover DG, Kniel KE. Viral inactivation in foods: A review of traditional and novel food-processing technologies. Compr Rev Food Sci Food Saf. 2010; 9: 3-20. https://doi.org/10.1111/j.1541-4337.2009.00092.x
  18. Maris P. Modes of action of disinfectants. Rev Sci. Tech Off Int Epiz. 1995; 14: 47-55. https://doi.org/10.20506/rst.14.1.829
  19. Turner RB, Biedermann KA, Morgan JM, Keswick B, Ertel KD, Barker MF. Efficacy of organic acids in hand cleansers for prevention of rhinovirus infections. Antimicrob Agents Chemother. 2004; 48: 2595-2598. https://doi.org/10.1128/AAC.48.7.2595-2598.2004
  20. Animal, Plant and Fisheries Quarantine and Inspection Agency (APFQIA). The guideline of the efficacy test for disinfectants. APFQIA Regulation No. 2013-34. Anyang: APFQIA; 2013.
  21. Karber G. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Exp Pathol Pharmakol. 1931; 162: 480-487. https://doi.org/10.1007/BF01863914
  22. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev. 1999; 12: 147-179.
  23. Jang Y, Lee G, Song J, Kim H, Jang B, Choe N. Time dependent efficacy change after dilution of the disinfectants against avian influenza virus. J Prev Vet Med. 2015; 39: 44-47.
  24. Yilmaz A, Heffels-Redmann U, Redmann T. Evaluation of the virucidal efficacy of two chemical disinfectants against avian influenza virus A at different temperatures. Arch Geflugelk. 2004; 68: 50-56.
  25. Heggers JP, Cottingham J, Gusman J, Reagor L, McCoy L, Carino E, et al. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: II. Mechanism of action and in vitro toxicity. J Altern Complement Med. 2002; 8: 333-340. https://doi.org/10.1089/10755530260128023
  26. International Chemical Safety Cards (ICSC). Citric acid. Available: http://www.cdc.gov/niosh/ipcsneng/neng0855.html [accessed 28 April 2016].
  27. Fiume Z. Final report on the safety assessment of malic acid and sodium malate. Int J Toxicol. 2001; 1 (20 Suppl): 47-55.
  28. European Medicines Agency (EMEA). The European Agency for the Evaluation of Medicinal Products, Veterinary Medicines Evaluation Unit, Committee for Veterinary Medicinal Products. Benzalkonium chloride - Summary Report. Available: http://www.ema.europa.eu/docs/en_GB/document_library/Maximum_Residue_Limits_-_Report/2009/11/WC500010967.pdf [accessed 28 April 2016].