DOI QR코드

DOI QR Code

A Study on the Development of System for the Micro-Volume Samples Analysis

극미량 시료분석을 위한 시스템 개발에 대한 연구

  • Lee, Sang Wook (Department of Mechanical Engineering, Korea Army Academy) ;
  • Lee, Jin Ho (Department of Mechanical Engineering, Korea Army Academy)
  • 이상욱 (육군 3 사관학교 기계공학과) ;
  • 이진호 (육군 3 사관학교 기계공학과)
  • Received : 2016.03.02
  • Accepted : 2016.05.04
  • Published : 2016.09.01

Abstract

Assay of biomolecules based on absorption spectroscopy is a sensitive, minute, quantitative, rapid and real-time process. UV-vis spectrometry has inherent advantages for use in analytical applications. To reduce the consumption of scarce samples, a new generation of miniaturized UV-vis spectrometric systems has been developed. This study determined empirically and through simulations the feasibility of assaying biomolecules by means of UV-vis spectrometry. This paper also reports a fully integrated portable micro-detection equipment system that utilizes a micro-optical path for analysis of micro-volume samples.

Keywords

References

  1. Wikipedia "Biomolecule," http://en.wikipedia.org/wiki/biomolecule, (Accessed 5 January 2016)
  2. Fodor, S. P. A., Read, J. L., Pirrung, M. C., Stryer, L., Lu, A. T., et al., "Light-Directed, Spatially Addressable Parallel Chemical Synthesis Science," Vol. 251, No. 4995, pp. 767-773, 1991. https://doi.org/10.1126/science.1990438
  3. Singh-Gasson, S., Green, R. D., Yue, Y., Nelson, C., Blattner, F., et al., "Maskless Fabrication of Light-Directed Oligonucleotide Microarrays Using a Digital Micromirror Array." Nature Biotechnology, Vol 17, No. 10, pp. 974-978, 1999. https://doi.org/10.1038/13664
  4. Haab, B. B., Dunham, M. J., and Brown, P. O., "Protein Microarrays for Highly Parallel Detection and Quantitation of Specific Proteins and Antibodies in Complex Solutions," Genome Biology, Vol. 2, No. 3, pp. 1-13, 2001.
  5. Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., et al., "Global Analysis of Protein Activities Using Proteome Chips," Science, Vol. 293, No. 5537, pp. 2101-2015, 2001. https://doi.org/10.1126/science.1062191
  6. Freundlieb, S. and Garner, J., "Chemical Microarrays: a Novel Approach to Drug Discovery," New Drugs, Vol. 3, pp. 54-60, 2002.
  7. Baker, D. D., Chu, M., Oza, U., and Rajgarhia, V., "The Value of Natural Products to Future Pharmaceutical Discovery," Natural Product Reports, Vol. 24, No. 6, pp. 1225-1244, 2007. https://doi.org/10.1039/b602241n
  8. Cai, Q. Y., Park, J., Heldsinger, D., Hsieh, M.-D., and Zellers, E. T., "Vapor Recognition with an Integrated Array of Polymer-Coated Flexural Plate Wave Sensors," Sensors and Actuators B: Chemical, Vol. 62, No. 2, pp. 121-130, 2000. https://doi.org/10.1016/S0925-4005(99)00381-0
  9. Cooper, M. A., "Label-Free Screening of Bio-Molecular Interactions," Analytical And Bioanalytical Chemistry, Vol. 377, No. 5, pp. 834-843, 2003. https://doi.org/10.1007/s00216-003-2111-y
  10. Homola, J., Yee, S. S., and Gauglitz, G., "Surface Plasmon Resonance Sensors: Review," Sensors and Actuators B: Chemical, Vol. 54, No. 1, pp. 3-15.1999. https://doi.org/10.1016/S0925-4005(98)00321-9
  11. Lofas, S., Malmqvist, M., Ronnberg, I., Stenberg, E., Liedberg, B., et al., "Bioanalysis with Surface Plasmon Resonance," Sensors and Actuators B: Chemical, Vol. 5, Nos. 1-4, pp. 79-84, 1991. https://doi.org/10.1016/0925-4005(91)80224-8
  12. Morhard, F., Pipper, J., Dahint, R., and Grunze, M., "Immobilization of Antibodies in Micropatterns for Cell Detection by Optical Diffraction," Sensors and Actuators B: Chemical, Vol. 70, No. 1, pp. 232-242, 2000. https://doi.org/10.1016/S0925-4005(00)00574-8
  13. Jin, G., Tengvall, P., Lundstrom, I., and Arwin, H., "A Biosensor Concept Based on Imaging Ellipsometry for Visualization of Biomolecular Interactions," Analytical Biochemistry, Vol. 232, No. 1, pp. 69-72, 1995. https://doi.org/10.1006/abio.1995.9959
  14. Huber, W., Barner, R., Fattinger, C., Hubscher, J., Koller, H., et al., "Direct Optical Immunosensing (Sensitivity and Selectivity)," Sensors and Actuators B: Chemical, Vol. 6, No. 1, pp. 122-126, 1992. https://doi.org/10.1016/0925-4005(92)80042-V
  15. Brecht, A. and Gauglitz, G., "Optical Probes and Transducer," Biosensors and Bioelectronics, Vol. 10, No. 9, pp. 923-936, 1995. https://doi.org/10.1016/0956-5663(95)99230-I
  16. Jenison, R., Yang, S., Haeberli, A., and Polisky, B., "Interference-Based Detection of Nucleic Acid Targets on Optically Coated Silicon," Nature Biotechnology, Vol. 19, No. 1, pp. 62-65, 2001. https://doi.org/10.1038/83530
  17. Lin, V. S.-Y., Motesharei, K., Dancil, K.-P. S., Sailor, M. J., and Ghadiri, M. R., "A Porous Silicon-Based Optical Interferometric Biosensor," Science, Vol. 278, No. 5339, pp. 840-843, 1997. https://doi.org/10.1126/science.278.5339.840
  18. Harsanyi, G., "Sensors in Biomedical Applications: Fundamentals, Technology and Applications," CRC Press, pp. 108-123, 1998.
  19. McCaig, T., "Extending the Use of Visible/Near-Infrared Reflectance Spectrophotometers to Measure Color of Food and Agricultural Products," Food Research International, Vol. 35, No. 8, pp. 731-736, 2002. https://doi.org/10.1016/S0963-9969(02)00068-6
  20. Kim, S., Yu, S. Y., Kim, S. K., and Bong, E. H., "Optical System Design for the UV Energy Measurement," Proc. of KSPE Spring Conference, pp. 489-490, 2011.