Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effect of Gentianae Macrophyllae Radix Pharmacopuncture at Hwando (GB30) on Neuropathic Pain in Tibial and Sural Nerve Transected Rats

환도(環跳)(GB30) 진구약침(秦艽藥鍼)이 신경병리성 통증 유발 흰쥐에 미치는 영향

  • Lee, Ook Jae (Cham Joeun Hospital) ;
  • Kim, Seon Wook (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Shin, Jeong Cheol (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University)
  • 이욱재 (참조은요양병원) ;
  • 김선욱 (동신대학교 한의과대학 침구의학교실) ;
  • 신정철 (동신대학교 한의과대학 침구의학교실)
  • Received : 2016.05.18
  • Accepted : 2016.08.26
  • Published : 2016.09.20
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Abstract

Objectives : The objective of this study was to investigate the effects of Gentianae Macrophyllae Radix pharmacopuncture (GP) at Hwando (GB30) in neuropathic pain induced rats. Methods : Neuropathic pain in rats was induced by tibial and sural nerve transection. The rat subjects were divided into 6 groups : normal (Nor, n = 5), control (Con, n = 5), neuropathic pain- induced injected at GB30 with 1 mg/kg GP (GP-A, n = 5), 5 mg/kg GP (GP-B, n = 5) and 20 mg/kg GP (GP-C, n = 5), and neuropathic pain-induced injected with 1mg/kg Tramadol (Tramadol, n＝5). Injections were administered 2 times a week for a total of 5 treatments. After each treatment plantar withdrawal response was measured and after all 5 treatments were completed c-fos, Bax, Bcl-2, mGlu5 and leukocytes in the blood were analyzed. Results : 1. Groups GP-A, GP-B and GP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia compared to the control group. 2. Groups GP-A, GP-B and GP-C showed a meaningful decrease in the expression of c-fos compared to the control group. 3. Groups GP-A and GP-C showed a meaningful increase in the expression of mGluR5 compared to the control group. 4. Groups GP-A, GP-B and GP-C showed a meaningful decrease in Bax/Bcl-2 ratio compared to the control group. Conclusion : These results suggest that Gentianae Macrophyllae Radix pharmacopuncture at Hwando (GB30) could decrease mechanical allodynia and could have analgesic and neuroprotective effects on the model of neuropathic pain.

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