Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effects of Ukgansan Pharmacopuncture at GB20 on Cognitive Impariment Induced by Focal Brain Injury in Rats

풍지(風池)(GB20) 억간산(抑肝散) 약침이 국소 뇌손상으로 유발된 흰쥐의 인지장애에 미치는 영향

  • Lee, Jung Hun (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Yang, Tae Jun (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Jeong, Sang Jun (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Wei, Tung Shuen (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University)
  • 이정훈 (동신대학교 한의과대학 침구의학교실) ;
  • 양태준 (동신대학교 한의과대학 침구의학교실) ;
  • 정상준 (동신대학교 한의과대학 침구의학교실) ;
  • 위통순 (동신대학교 한의과대학 침구의학교실)
  • Received : 2016.08.12
  • Accepted : 2016.09.06
  • Published : 2016.09.20
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Abstract

Objectives : This research was performed to investigate the effects of Ukgansan pharmacopuncture(U-PA) of focal brain ischemia induced by middle cerebral artery occlusion(MCAO) in rats. Methods : The subjects were divided into 5 groups : A control group, acupuncture group, pharmacopuncture group U-PA1($2.571mg/250g/40{\mu}{\ell}$), pharmacopuncture group U-PA2($6.428mg/250g/40{\mu}{\ell}$), and pharmacopuncture group U-PA3($12.855mg/250g/40{\mu}{\ell}$). The focal brain ischemia was induced by intraluminal filament insertion into the middle cerebral artery. After 3 days of MCAO, Ukgansan(UGS) pharmacopuncture treatment was performed on the GB20, and the day after being treated with pharmacopuncture, the Morris water maze test was carried out by the assigned group. The series of processes were treated 6 times. Thereafter Bax, Bcl-2, Bax/Bcl-2 ratio, mGluR5, density of neuronal cell, and ChAT were measured. Results : The results were as follows. 1. The intensity of Bax significantly decreased in the U-PA1, U-PA2, U-PA3 groups. 2. The Bax/Bcl-2 ratio significantly decreased in the U-PA3 group compared with the control group. 3. The neuroprotective effect on the hippocampal CA1 significantly increased in the U-PA1, U-PA2, U-PA3 groups compared with the control group. 4. The density of ChAT in the hippocampal CA1 significantly increased in the U-PA1, U-PA2, U-PA3 groups compared with the control group. Conclusion : These results suggest that UGS pharmacopuncture may have anti-apoptotic and neuroprotective effects on focal cerebral ischemia caused by intraluminal filament insertion into the middle cerebral artery in rats.

Keywords

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