Environmental Engineering Research

  • 제21권3호
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  • Pages.265-275
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  • 2016
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  • 1226-1025(pISSN)
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  • 2005-968X(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
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Anaerobic digestate as a nutrient medium for the growth of the green microalga Neochloris oleoabundans

  • Abu Hajar, Husam A. (Department of Civil Engineering, Ohio University) ;
  • Guy Riefler, R. (Department of Civil Engineering, Ohio University) ;
  • Stuart, Ben J. (Department of Civil & Environmental Engineering, Old Dominion University)
  • 투고 : 2016.01.12
  • 심사 : 2016.04.09
  • 발행 : 2016.09.30
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초록

In this study, the microalga Neochloris oleoabundans was cultivated in a sustainable manner using diluted anaerobic digestate to produce biomass as a potential biofuel feedstock. Prior to microalgae cultivation, the anaerobic digestate was characterized and several pretreatment methods including hydrogen peroxide treatment, filtration, and supernatant extraction were investigated and their impact on the removal of suspended solids as well as other organic and inorganic matter was evaluated. It was found that the supernatant extraction was the most convenient pretreatment method and was used afterwards to prepare the nutrient media for microalgae cultivation. A bench-scale experiment was conducted using multiple dilutions of the supernatant and filtered anaerobic digestate in 16 mm round glass vials. The results indicated that the highest growth of the microalga N. oleoabundans was achieved with a total nitrogen concentration of 100 mg N/L in the 2.29% diluted supernatant in comparison to the filtered digestate and other dilutions.

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참고문헌

  1. Gouveia L, Marques A, Da Silva T, Reis A. Neochloris oleoabundans UTEX #1185: A suitable renewable lipid source for biofuel production. J. Ind. Microbiol. Biotechnol. 2009;36:821-826. https://doi.org/10.1007/s10295-009-0559-2
  2. Gouveia L, Oliveira A. Microalgae as a raw material for biofuels production. J. Ind. Microbiol. Biotechnol. 2009;36:269-274. https://doi.org/10.1007/s10295-008-0495-6
  3. Levine RB, Costanza-Robinson MS, Spatafora GA. Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production. Biomass Bioenerg. 2009;35:40-49.
  4. Pruvost J, Van Vooren G, Cogne G, Legrand J. Investigation of biomass and lipids production with Neochloris oleoabundans in photobioreactor. Bioresour. Technol. 2009;100:5988-5995. https://doi.org/10.1016/j.biortech.2009.06.004
  5. Gour RS, Kant A, Chauhan RS. Screening of micro algae for growth and lipid accumulation properties. J. Algal. Biomass Utln. 2014;5:38-46.
  6. Li Y, Horsman M, Wang B, Wu N, Lan C. Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl. Microbiol. Biotechnol. 2008;81:629-636. https://doi.org/10.1007/s00253-008-1681-1
  7. Meti BS, Sailaja B. Treatment of sugar process waste water and biogas production using algal biomass. Int. J. Eng. Res. Technol. 2014;3:61-67.
  8. James SC, Boriah V. Modeling algae growth in an open-channel raceway. J. Comput. Biol. 2010;17:895-906. https://doi.org/10.1089/cmb.2009.0078
  9. Terry KT, Raymond LP. System design for the autotrophic production of microalgae. Enzyme Microb. Technol. 1985;7:474-487. https://doi.org/10.1016/0141-0229(85)90148-6
  10. Santos AM, Lamers PP, Janssen M, Wijffels RH. Biomass and lipid productivity of Neochloris oleoabundans under alkaline-saline conditions. Algal Res. 2013;2:204-211. https://doi.org/10.1016/j.algal.2013.04.007
  11. Da Silva TL, Reis A, Medeiros R, Oliveira AC, Gouveia L. Oil production towards biofuel from autotrophic microalgae semicontinuous cultivations monitorized by flow cytometry. Appl. Biochem. Biotech. 2009;159:568-578. https://doi.org/10.1007/s12010-008-8443-5
  12. Giovanardi M, Baldisserotto C, Ferroni L, Longoni P, Cella R, Pancaldi S. Growth and lipid synthesis promotion in mixotrophic Neochloris oleoabundans (Chlorophyta) cultivated with glucose. Protoplasma 2014;251:115-125. https://doi.org/10.1007/s00709-013-0531-x
  13. Urreta I, Ikaran Z, Janices I, et al. Revalorization of Neochloris oleoabundans biomass as source of biodiesel by concurrent production of lipids and carotenoids. Algal Res. 2014;5:16-22. https://doi.org/10.1016/j.algal.2014.05.001
  14. Wang B, Lan CQ. Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent. Bioresour. Technol. 2011;102:639-644.
  15. Yang Y, Mininberg B, Tarbet A, Weathers P. At high temperature lipid production in Ettlia oleoabundans occurs before nitrate depletion. Appl. Microbiol. Biot. 2013;97:2263-2273. https://doi.org/10.1007/s00253-012-4671-2
  16. Choi HJ, Lee SM. Effects of microalgae on the removal of nutrients from wastewater: Various concentrations of Chlorella vulgaris. Environ. Eng. Res. 2012;17:S3-S8. https://doi.org/10.4491/eer.2012.17.1.003
  17. Tam NFY, Wong YS. The comparison of growth and nutrient removal efficiency of Chlorella pyrenoidosa in settled and activated sewages. Environ. Pollut. 1990;65:93-108. https://doi.org/10.1016/0269-7491(90)90177-E
  18. Park K, Lim B, Lee K. Growth of microalgae in diluted process water of the animal wastewater treatment plant. Water Sci. Technol. 2009;59:2111-2116. https://doi.org/10.2166/wst.2009.233
  19. Tam NFY, Wong YS. Effect of ammonia concentrations on growth of Chlorella vulgaris and nitrogen removal from media. Bioresour. Technol. 1996;57:45-50. https://doi.org/10.1016/0960-8524(96)00045-4
  20. Park J, Jin HF, Lim BR, Park KY, Lee K. Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresour. Technol. 2010;101:8649-8657. https://doi.org/10.1016/j.biortech.2010.06.142
  21. Franchino M, Comino E, Bona F, Riggio VA. Growth of three microalgae strains and nutrient removal from an agro-zootechnical digestate. Chemosphere 2013;92:738-744. https://doi.org/10.1016/j.chemosphere.2013.04.023
  22. Yang Y, Xu J, Vail D, Weathers P. Ettlia oleoabundans growth and oil production on agricultural anaerobic waste effluents. Bioresour. Technol. 2011;102:5076-5082. https://doi.org/10.1016/j.biortech.2011.02.014
  23. Sturm BS, Lamer SL. An energy evaluation of coupling nutrient removal from wastewater with algal biomass production. Appl. Energ. 2011;88:3499-3506. https://doi.org/10.1016/j.apenergy.2010.12.056
  24. Clesceri L, Greenberg A, Eaton A. Standard Methods for the Examination of Water and Wastewater, 20th ed. Washington D.C: American Public Health Association; 1998.
  25. Crittenden JC, Trussell RR, Hand DW, Howe KJ, Tchobanoglous G. Water treatment principles and design. Hoboken, NJ: John Wiley & Sons; 2005.
  26. Ksibi M. Chemical oxidation with hydrogen peroxide for domestic wastewater treatment. Chem. Eng. J. 2006;119:161-165. https://doi.org/10.1016/j.cej.2006.03.022
  27. James DE. Culturing algae. Carolina Biological Supply Company; 1978.
  28. Forster CF. The surface of activated sludge particles in relation to their settling characteristics. Water Res. 1968;2:767-776. https://doi.org/10.1016/0043-1354(68)90011-0
  29. Pere J, Alen R, Viikari L, Eriksson L. Characterization and dewatering of activated sludge from the pulp and paper industry. Water Sci. Technol. 1993;28:193-201.
  30. Liao BQ, Allen DG, Droppo IG, Leppard GG, Liss SN. Surface properties of sludge and their role in bioflocculation and settleability. Water Res. 2001;35:339-350. https://doi.org/10.1016/S0043-1354(00)00277-3
  31. Su B, Qu Z, Song Y, Jia L, Zhu J. Investigation of measurement methods and characterization of zeta potential for aerobic granular sludge. J. Environ. Chem. Eng. 2014;2:1142-1147. https://doi.org/10.1016/j.jece.2014.03.006
  32. Morgan JW, Forster CF, Evison L. A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges. Water Res. 1990;24:743-750. https://doi.org/10.1016/0043-1354(90)90030-A
  33. Roudsari FP, Mehrnia MR, Asadi A, Moayedi Z, Ranjbar R. Effect of microalgae/activated sludge ratio on cooperative treatment of anaerobic effluent of municipal wastewater. Appl. Biochem. Biotech. 2014;172:131-140. https://doi.org/10.1007/s12010-013-0480-z
  34. Travieso L, Benitez F, Sanchez E, Borja R, Martin A, Colmenarejo MF. Batch mixed culture of Chlorella vulgaris using settled and diluted piggery waste. Ecol. Eng. 2006;28:158-165. https://doi.org/10.1016/j.ecoleng.2006.06.001
  35. Carlsson B. An introduction to sedimentation theory in wastewater treatment. Systems and Control Group, Uppsala University; 1998.
  36. Mata TM, Martins AA, Caetano NS. Microalgae for biodiesel production and other applications: A review. Renew. Sust. Energ. Rev. 2010;14:217-232. https://doi.org/10.1016/j.rser.2009.07.020
  37. Menetrez MY. An overview of algae biofuel production and potential environmental impact. Environ. Sci. Technol. 2012;46:7073-7085. https://doi.org/10.1021/es300917r
  38. Rodolfi L, Zittelli GC, Bassi N, et al. Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol. Bioeng. 2009;102:100-112. https://doi.org/10.1002/bit.22033
  39. Sheehan J, Dunahay T, Benemann J, Roessler P. A look back at the U.S. Department of Energy's Aquatic Species Program-Biodiesel from algae. U.S. Report NREL/TP-580-24190. National Renewable Energy Laboratory: Golden CO; 1998.

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