Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of combined sodium hyaluronate and carboxymethyl cellulose on ocular surface in rat dry eye model

  • Moon, Jong-Gab (Department of Veterinary surgery, College of Veterinary Medicine, Kyungpook National University) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Kwon, Young-Sam (Department of Veterinary surgery, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2016.01.28
  • Accepted : 2016.07.01
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to evaluate three different mixed formulations of sodium hyaluronate (SH) and carboxymethyl cellulose (CMC) using a low-humidity air flow-induced rat dry eye model and determine the most suitable mixture. The total thickness of the cornea, corneal epithelial thickness, corneal stroma thickness, damaged corneal epithelium percentage region, thickness of the bulbar conjunctiva epithelium, number of goblet cells, goblet cell occupation percentage region, and damaged bulbar conjunctiva epithelium percentage region were measured by histomorphological evaluation. After 5 h exposure to drying airflow, the thickness of the cornea and conjunctiva was decreased with desquamation of the corneal and conjunctiva epithelium. However, these dry eye symptoms were markedly inhibited by treatment with the reference and test formulations. More favorable effects on decreased thickness were detected in response to the CMC than the SH. However, SH had a greater protective effect against corneal and conjunctiva epithelial damage. The application of a mixture of 0.1% SH and 0.2% CMC showed more favorable effects on the corneal and conjunctival damage and the stabilization of the ocular surface than SH or CMC alone.

Keywords

References

  1. Ahee JA, Kaufman SC, Samuel MA, Bogorad D, Wee C. Decreased incidence of epithelial defects during laser in situ keratomileusis using intraoperative nonpreserved carboxymethylcellulose sodium 0.5% solution. J Cataract Refract Surg 2002, 28, 1651-1654. https://doi.org/10.1016/S0886-3350(01)01348-7
  2. Albietz JM, Lenton LM, McLennan SG, Earl ML. A comparison of the effect of refresh plus and bion tears on dry eye symptoms and ocular surface health in myopic LASIK patients. CLAO J 2002, 28, 96-100.
  3. Arslan E, Talih T, Oz B, Halaclar B, Caglayan K, Sipahi M. Comparison of lovastatin and hyaluronic acid/carboxymethyl cellulose on experimental created peritoneal adhesion model in rats. Int J Surg 2014, 12, 120-124. https://doi.org/10.1016/j.ijsu.2013.11.010
  4. Barabino S, Dana MR. Animal models of dry eye: a critical assessment of opportunities and limitations. Invest Ophthalmol Vis Sci 2004, 45, 1641-1646. https://doi.org/10.1167/iovs.03-1055
  5. Bashir S, Ananth CV, Lewin SN, Burke WM, Lu YS, Neugut AI, Herzog TJ, Hershman DL, Wright JD. Utilization and safety of sodium hyaluronate-carboxymethylcellulose adhesion barrier. Dis Colon Rectum 2013, 56, 1174-1184. https://doi.org/10.1097/DCR.0b013e31829ec889
  6. Brewitt H, Sistani F. Dry eye disease: the scale of the problem. Surv Ophthalmol 2001, 45 (Suppl 2), S199-202. https://doi.org/10.1016/S0039-6257(00)00202-2
  7. Burgalassi S, Panichi L, Chetoni P, Saettone MF, Boldrini E. Development of a simple dry eye model in the albino rabbit and evaluation of some tear substitutes. Ophthalmic Res 1999, 31, 229-235. https://doi.org/10.1159/000055537
  8. Caglayan EK, Caglayan K, Erdogan N, Cinar H, Gungor B. Preventing intraperitoneal adhesions with ethyl pyruvate and hyaluronic acid/carboxymethylcellulose: a comparative study in an experimental model. Eur J Obstet Gynecol Reprod Biol 2014, 181, 1-5. https://doi.org/10.1016/j.ejogrb.2014.07.004
  9. Caglayan K, Gungor B, Cinar H, Erdogan NY, Koca B. Preventing intraperitoneal adhesions with linezolid and hyaluronic acid/carboxymethylcellulose: a comparative study in cecal abrasion model. Am J Surg 2014, 208, 106-111. https://doi.org/10.1016/j.amjsurg.2012.05.038
  10. DeLuise VP, Peterson WS. The use of topical Healon tears in the management of refractory dry-eye syndrome. Ann Ophthalmol 1984, 16, 823-824.
  11. Donshik PC, Nelson JD, Abelson M, McCulley JP, Beasley C, Laibovitz RA. Effectiveness of BION tears, Cellufresh, Aquasite, and Refresh Plus for moderate to severe dry eye. Adv Exp Med Biol 1998, 438, 753-760. https://doi.org/10.1007/978-1-4615-5359-5_106
  12. Dursun D, Wang M, Monroy D, Li DQ, Lokeshwar BL, Stern ME, Pflugfelder SC. A mouse model of keratoconjunctivitis sicca. Invest Ophthalmol Vis Sci 2002, 43, 632-638.
  13. Fujihara T, Nagano T, Nakamura M, Shirasawa E. Establishment of a rabbit short-term dry eye model. J Ocul Pharmacol Ther 1995, 11, 503-508. https://doi.org/10.1089/jop.1995.11.503
  14. Grene RB, Lankston P, Mordaunt J, Harrold M, Gwon A, Jones R. Unpreserved carboxymethylcellulose artificial tears evaluated in patients with keratoconjunctivitis sicca. Cornea 1992, 11, 294-301. https://doi.org/10.1097/00003226-199207000-00004
  15. Higuchi A, Ueno R, Shimmura S, Suematsu M, Dogru M, Tsubota K. Albumin rescues ocular epithelial cells from cell death in dry eye. Curr Eye Res 2007, 32, 83-88. https://doi.org/10.1080/02713680601147690
  16. Inoue M, Katakami C. The effect of hyaluronic acid on corneal epithelial cell proliferation. Invest Ophthalmol Vis Sci 1993, 34, 2313-2315.
  17. Johnson ME, Murphy PJ, Boulton M. Effectiveness of sodium hyaluronate eyedrops in the treatment of dry eye. Graefes Arch Clin Exp Ophthalmol 2006, 244, 109-112. https://doi.org/10.1007/s00417-005-0028-1
  18. Lenton LM, Albietz JM. Effect of carmellose-based artificial tears on the ocular surface in eyes after laser in situ keratomileusis. J Refract Surg 1999, 15 (2 Suppl), S227-231.
  19. Maitchouk DY, Beuerman RW, Ohta T, Stern M, Varnell RJ. Tear production after unilateral removal of the main lacrimal gland in squirrel monkeys. Arch Ophthalmol 2000, 118, 246-252. https://doi.org/10.1001/archopht.118.2.246
  20. Nakamura S, Shibuya M, Saito Y, Nakashima H, Saito F, Higuchi A, Tsubota K. Protective effect of D-${\beta}$-hydroxybutyrate on corneal epithelia in dry eye conditions through suppression of apoptosis. Invest Ophthalmol Vis Sci 2003, 44, 4682-4688. https://doi.org/10.1167/iovs.03-0198
  21. Nehez L, Vodros D, Axelsson J, Tingstedt B, Lindman B, Andersson R. Prevention of postoperative peritoneal adhesions: effects of lysozyme, polylysine and polyglutamate versus hyaluronic acid. Scand J Gastroenterol 2005, 40, 1118-1123. https://doi.org/10.1080/00365520510023332
  22. Nelson JD, Farris RL. Sodium hyaluronate and polyvinyl alcohol artificial tear preparations. A comparison in patients with keratoconjunctivitis sicca. Arch Ophthalmol 1988, 106, 484-487. https://doi.org/10.1001/archopht.1988.01060130530029
  23. Ryan CK, Sax HC. Evaluation of a carboxymethylcellulose sponge for prevention of postoperative adhesions. Am J Surg 1995, 169, 154-159. https://doi.org/10.1016/S0002-9610(99)80125-1
  24. Stuart JC, Linn JG. Dilute sodium hyaluronate (Healon) in the treatment of ocular surface disorders. Ann Ophthalmol 1985, 17, 190-192.
  25. Wurster SH, Bonet V, Mayberry A, Hoddinott M, Williams T, Chaudry IH. Intraperitoneal sodium carboxymethylcellulose administration prevents reformation of peritoneal adhesions following surgical lysis. J Surg Res 1995, 59, 97-102. https://doi.org/10.1006/jsre.1995.1138
  26. Wysenbeek YS, Loya N, Ben Sira I, Ophir I, Ben Shaul Y. The effect of sodium hyaluronate on the corneal epithelium. An ultrastructural study. Invest Ophthalmol Vis Sci 1988, 29, 194-199.