Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Hens immunized with live attenuated Salmonella strains expressing virulence-associated genes in avian pathogenic Escherichia coli passively transfer maternal antibodies to chicks

  • Won, Gayeon (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, John Hwa (College of Veterinary Medicine, Chonbuk National University)
  • Received : 2016.05.08
  • Accepted : 2016.07.11
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated whether maternal antibodies (mAbs) elicited by dams immunized with recombinant vaccine candidates against avian pathogenic Escherichia coli (APEC) can passively confer protective immunity to chicks. In the present study, pBP244 plasmids carrying selected antigens of APEC were transformed into Salmonella Typhimurium JOL912, which was used as a vaccine candidate against APEC. The hens were immunized with the vaccine candidates using prime or booster doses. The levels of IgG and sIgA specific to the selected antigens increased significantly following prime immunization. To evaluate the persistence of passively transferred mAbs, the levels of IgY and IgA were determined in egg yolks and whites, respectively. The eggs from the immunized group showed consistently increased levels of IgY and IgA until week 16 post-laying (PL) and week 8 PL, respectively, relative to the control group. The presence of mAbs was observed in chicks that hatched from the hens, and titers of plasma IgY were consistently raised in those from the immunized hens by day 14 post-hatching. Further, chicks from the immunized hens were protected from challenge with a virulent APEC strain, whereas those from non-immunized hens showed acute mortality.

Keywords

References

  1. Alvarez J, Sota M, Vivanco AB, Perales I, Cisterna R, Rementeria A, Garaizar J. Development of a multiplex PCR technique for detection and epidemiological typing of Salmonella in human clinical samples. J Clin Microbiol 2004, 42, 1734-1748. https://doi.org/10.1128/JCM.42.4.1734-1738.2004
  2. Brambell FWR. The transmission of immunity in birds. In: The Transmission of Passive Immunity from Mother to Young. North-Holland, London, 1970.
  3. Clem LW, Small PA Jr. Phylogeny of immunoglobulin structure and function. I. Immunoglobulins of the lemon shark. J Exp Med 1967, 125, 893-920. https://doi.org/10.1084/jem.125.5.893
  4. Dho-Moulin M, Fairbrother JM. Avian pathogenic Escherichia coli (APEC). Vet Res 1999, 30, 299-316.
  5. Dziva F, Stevens MP. Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts. Avian Pathol 2008, 37, 355-366. https://doi.org/10.1080/03079450802216652
  6. Gantois I, Ducatelle R, Timbermont L, Boyen F, Bohez L, Haesebrouck F, Pasmans F, van Immerseel F. Oral immunisation of laying hens with the live vaccine strains of TAD Salmonella $vac^{(R)}$ E and TAD Salmonella $vac^{(R)}$ T reduces internal egg contamination with Salmonella Enteritidis. Vaccine 2006, 24, 6250-6255. https://doi.org/10.1016/j.vaccine.2006.05.070
  7. Gogal RM Jr, Ahmed SA, Larsen CT. Analysis of avian lymphocyte proliferation by a new, simple, nonradioactive assay (lympho-pro). Avian Dis 1997, 41, 714-725. https://doi.org/10.2307/1592166
  8. Grindstaff JL, Brodie ED 3rd, Ketterson ED. Immune function across generations: integrating mechanism and evolutionary process in maternal antibody transmission. Proc Biol Sci 2003, 270, 2309-2319. https://doi.org/10.1098/rspb.2003.2485
  9. Heller ED, Leitner H, Drabkin N, Melamed D. Passive immunisation of chicks against Escherichia coli. Avian Pathol 1990, 19, 345-354. https://doi.org/10.1080/03079459008418685
  10. Kang HY, Srinivasan J, Curtiss R 3rd. Immune responses to recombinant pneumococcal PspA antigen delivered by live attenuated Salmonella enterica serovar Typhimurium vaccine. Infect Immun 2002, 70, 1739-1749. https://doi.org/10.1128/IAI.70.4.1739-1749.2002
  11. Kariyawasam S, Wilkie BN, Gyles CL. Resistance of broiler chickens to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies. Vet Microbiol 2004, 98, 273-284. https://doi.org/10.1016/j.vetmic.2003.10.022
  12. Kariyawasam S, Wilkie BN, Hunter DB, Gyles CL. Systemic and mucosal antibody responses to selected cell surface antigens of avian pathogenic Escherichia coli in experimentally infected chickens. Avian Dis 2002, 46, 668-678. https://doi.org/10.1637/0005-2086(2002)046[0668:SAMART]2.0.CO;2
  13. Kim SW, Kim YH, Yoo AY, Yu JE, Hur J, Lee JH, Cha J, Kang HY. Development of a protein secretion system with the application of sec-dependent protein secretion components. J Microbiol Biotechnol 2007, 17, 1316-1323.
  14. Kim SW, Moon KH, Baik HS, Kang HY, Kim SK, Bahk JD, Hur J, Lee JH. Changes of physiological and biochemical properties of Salmonella enterica serovar Typhimurium by deletion of cpxR and lon genes using allelic exchange method. J Microbiol Methods 2009, 79, 314-320. https://doi.org/10.1016/j.mimet.2009.09.025
  15. Leslie GA, Martin LN. Modulation of immunoglobulin ontogeny in the chicken: effect of purified antibody specific for ${\mu}$ chain on IgM, IgY, and IgA production. J Immunol 1973, 110, 959-967.
  16. Loeken MR, Roth TF. Analysis of maternal IgG subpopulations which are transported into the chicken oocyte. Immunology 1983, 49, 21-28.
  17. Polson A, Coetzer T, Kruger J, von Maltzahn E, van der Merwe KJ. Improvements in the isolation of IgY from the yolks of eggs laid by immunized hens. Immunol Invest 1985, 14, 323-327. https://doi.org/10.3109/08820138509022667
  18. Porter RE Jr, Holt PS. Use of a pilocarpine-based lavage procedure to study secretory immunoglobulin concentration in the alimentary tract of White Leghorn chickens. Avian Dis 1992, 36, 529-536. https://doi.org/10.2307/1591745
  19. Rana N, Kulshreshtha RC. Cell-mediated and humoral immune responses to a virulent plasmid-cured mutant strain of Salmonella enterica serotype Gallinarum in broiler chickens. Vet Microbiol 2006, 115, 156-162. https://doi.org/10.1016/j.vetmic.2006.01.011
  20. Sharma JM, Dohms JE, Metz AL. Comparative pathogenesis of serotype 1 and variant serotype 1 isolates of infectious bursal disease virus and their effect on humoral and cellular immune competence of specific-pathogen-free chickens. Avian Dis 1989, 33, 112-124. https://doi.org/10.2307/1591076
  21. Sirard JC, Niedergang F, Kraehenbuhl JP. Live attenuated Salmonella: a paradigm of mucosal vaccines. Immunol Rev 1999, 171, 5-26. https://doi.org/10.1111/j.1600-065X.1999.tb01340.x
  22. Smith NC, Wallach M, Miller CM, Morgenstern R, Braun R, Eckert J. Maternal transmission of immunity to Eimeria maxima: enzyme-linked immunosorbent assay analysis of protective antibodies induced by infection. Infect Immun 1994, 62, 1348-1357.
  23. Son AP. Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. Immunol Invest 1990, 19, 253-258. https://doi.org/10.3109/08820139009041840
  24. Sztein MB. Cell-mediated immunity and antibody responses elicited by attenuated Salmonella enterica serovar Typhi strains used as live oral vaccines in humans. Clin Infect Dis 2007, 45 (Suppl 1), S15-19. https://doi.org/10.1086/518140