DOI QR코드

DOI QR Code

Synergistic effect of co-inoculation with phosphate-solubilizing bacteria

  • Park, Jin-Hee (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Heon-Hak (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Han, Chang-Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yoo, Jeoung-Ah (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2016.05.03
  • Accepted : 2016.07.28
  • Published : 2016.09.30

Abstract

The synergistic effect on phosphate solubilization of single- and co-inoculation of two phosphate solubilizing bacteria, Burkholderia anthina PSB-15 and Enterobacter aerogenes PSB-16, was assessed in liquid medium and green gram plants. Co-inoculation of two strains was found to release the highest content of soluble phosphorus ($519{\mu}g\;mL^{-1}$) into the medium, followed by single inoculation of Burkholderia strain ($492{\mu}g\;mL^{-1}$) and Enterobacter strain ($483{\mu}g\;mL^{-1}$). However, there was no significant difference between single inoculation of bacterial strain and co-inoculation of two bacterial strains in terms of phosphorous release. The highest pH reduction, organic acid production, and glucose consumption were observed in the culture medium co-inoculated with PSB-15 and PSB-16 strains rather than that of single inoculation. Based on the plant growth promotion bioassay, co-inoculated mung bean seedlings recorded 9% and 8% higher shoot and root growth, respectively, compared to the control. Therefore, in conclusion, co-inoculation of the strains B. anthina and E. aerogenes displayed better performance in stimulating plant growth than inoculation of each strain alone. However, considering the short assessment period of the present study, we recommend engaging in further work under field conditions in order to test the suitability of these strains as bio-inoculants.

Keywords

References

  1. Ali B, Sabri AN, Hasnain S. 2010. Rhizobacteial potential to alter auxin content and growth of Vigna radiate (L.). World Journal of Microbiology and Biotechnology 26:1379-1384. https://doi.org/10.1007/s11274-010-0310-1
  2. Ahuja A, Ghosh SB, D'Souza SF. 2007. Isolation of starch utilizing, phosphate solubilization fungus medium and its characterization. Bioresource Technology 98:3408-3411. https://doi.org/10.1016/j.biortech.2006.10.041
  3. Bras RR, Nahas E. 2012. Synergistic action of both Aspergillus niger and Burkholderia cepacea in co-culture increases phosphate solubilization in growth medium. FEMS Microbiology Letters 332:84-90. https://doi.org/10.1111/j.1574-6968.2012.02580.x
  4. Chen Z, Ma S, Liu LL. 2008. Studies on phosphorus solubilizing activity of a strain of phospho bacteria isolated from chestnut type soil in China. Bioresource Technology 99:6702-6707. https://doi.org/10.1016/j.biortech.2007.03.064
  5. Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34:33-41. https://doi.org/10.1016/j.apsoil.2005.12.002
  6. Chaiharn M, Lumyong S. 2009. Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Nothern Thailand. World Journal of Microbiology and Biotechnology 25:305-314. https://doi.org/10.1007/s11274-008-9892-2
  7. Dey R, Pal KK, Bhatt DM, Chauhan SM. 2004. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiological Research 159:371-394. https://doi.org/10.1016/j.micres.2004.08.004
  8. Edi-Premono M, Moawad A, Vleck PLG. 1996. Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indian Journal of Crop Science 11:13-23.
  9. EI-Yazeid AA, Abou-Aly HE. 2011. Enhancing growth, productivity and quality of tomato plants using phosphate solubilizing microorganisms. Australian Journal of Basic and Applied Sciences 5:371-379.
  10. Fernandez LA, Zalba P, Gomez MA, Sagardoy MA. 2007. Phosphate solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions. Biology and Fertility of Soils 43:805-809. https://doi.org/10.1007/s00374-007-0172-3
  11. Frossard E, Condron LM, Oberson A, Sinaj S, Fardeau JC. 2000. Processes governing phosphorus availability in temperate soils. Journal of Environmental Quality 29:12-53.
  12. Ghanem KHM, Abbas EE. 2009. Improvement of mung bean growth and productivity in salinity-affected soil after seed inoculation with phosphate-dissolving bacteria. African Crop Science conference proceedings 9:385-389.
  13. Gaur AC. 1990. Phosphate solubilizing microorganisms as biofertilizers. Omega Scientific Publishers, New Delhi, India.
  14. Halder AK, Mishra AK, Bhattacharya P, Chakrabarty PK. 1991. Solubilization of inorganic phosphates by Bradyrhizobium. Indian Journal of Experimental Biology. 29:28-31.
  15. Hameeda B, Harini G, Rupela OP, Wani SP, Reddy G. 2006. Growth promotion of maize by phosphate solubilizing bacteria isolated from composts and macrofauna. Microbiological Research 163:234-242.
  16. Hariprasad P, Niranjana SR. 2009. Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant and Soil 316:13-24. https://doi.org/10.1007/s11104-008-9754-6
  17. Jackson ML. 1973. Soil Chemical Analysis. Prentice-Hall of India Pvt. Ltd., New Delhi, India.
  18. Jain R, Saxena J, Sharma V. 2012. Effect of phosphate solubilizing fungi Aspergillus awamori S29 on mungbean (Vigna radiata cv. RMG 492) growth. Folia microbiologica DOI 10.1007/s12223-012-0167-9.
  19. Khalid A, Arshad M, Zahir ZA. 2004. Screening plant growth promoting rhizobacteria for improving growth and yield of wheat. Journal of Applied Microbiology 96:473-480. https://doi.org/10.1046/j.1365-2672.2003.02161.x
  20. Khalimi K, Suprapta DN, Nitta Y. 2012. Effect of Pantoea agglomerans on growth promotion and yield of rice. Journal of Agricultural Science Research 2:240-249.
  21. Khan MS, Zaidi A, Wani P. 2007. Role of phosphate solubilizing microorganisms in sustainable agriculture-A review. Agronomy for Sustainable Development 27: 29-43. https://doi.org/10.1051/agro:2006011
  22. Kpomblekou K, Tabatabai MA, 1994. Effect of organic acids on release of phosphorus from phosphate rocks. Soil Science 158:442-453. https://doi.org/10.1097/00010694-199415860-00006
  23. Kumar S, Tamura K, Jakobsen IB, Nei M. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244-1245. https://doi.org/10.1093/bioinformatics/17.12.1244
  24. Kumar V, Behi RK, Narula N. 2001. Establishment of phosphate solubilizing strains of Azotobacter chroococcum in the rhizosphere and their effect on wheat cultivars under greenhouse conditions. Microbiological Research 156:87-93. https://doi.org/10.1078/0944-5013-00081
  25. Lin TF, Huang HI, Shen FT, Young CC. 2006. The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-A174. Bioresource Technology 97:957-960. https://doi.org/10.1016/j.biortech.2005.02.017
  26. Linu MS, Stephen J, Jisha MS. 2009. Phosphate solubilizing Gluconacetobacter sp., Burkholderia sp. and their potential interaction with cowpea (Vigna unguiculata (L.) Walp.). International Journal of Agricultural Research 4:79-87. https://doi.org/10.3923/ijar.2009.79.87
  27. Maliha R, Samina K, Najma A, Sadia A, Farooq L. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms under in vitro conditions. Pakistan Journal of Biological Sciences 7:187-196. https://doi.org/10.3923/pjbs.2004.187.196
  28. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Journal of Analytical Chemistry 31:426-428. https://doi.org/10.1021/ac60147a030
  29. Mundra S, Arora R, Stobdan T. 2011. Solubilization of insoluble inorganic phosphates by a novel temperature, pH, and salt tolerant yeast, Rhodotorula sp. PS4, isolated from seabuckthorn rhizosphere, growing in cold desert of Ladakh, India. World Journal of Microbiology and Biotechnology 27:2387-2396. https://doi.org/10.1007/s11274-011-0708-4
  30. Nautiyal CS, Bhadauria S, Kumar P, Lal H, Mondal R, Verma D. 2000. Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS microbiology letters.182: 291-296. https://doi.org/10.1111/j.1574-6968.2000.tb08910.x
  31. Nautiyal CS. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170:265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  32. Podile AR, Kishor GK. 2006. Plant growth promoting rhizobacteria. Plant associated bacteria pp.195-230. Gnanamanickam, s.s(eds.). springer, Dordrecht.
  33. Ponmurugan P, Gopi C. 2006. In vitro production of growth regulators of phosphatase activity by phosphate solubilizing bacteria. African Journal of Biotechnology 5:348-350.
  34. Qureshi MA, Shakir MA, Iqbal A, Akhtar N, Khan A. 2011. Co-inoculation of phosphate solubilizing bacteria and rhizobia for improving growth and yield of mung bean (Vigna radiate L.). Journal of Animal and Plant Sciences 21:491-497.
  35. Rangarajan S, Saleena LM, Vasudevan P, Nair S. 2003. Biological suppression of rice disease by Pseudomonas sp. under saline soil condition. Plant Soil 251:73-82. https://doi.org/10.1023/A:1022950811520
  36. Reyes I, Bernier L, Antoun H. 2002. Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum. Microbial Ecology 44:39-48. https://doi.org/10.1007/s00248-002-1001-8
  37. SAS. 1999. SAS/STAT User's Guide Version 8. SAS, Cary, NC.
  38. Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  39. Singh CP, Amberger A. 1991. Solubilization and availability of phosphorous during decomposition of rock phosphate enriched straw and urine. Biological Agriculture and Horticulture 7:1-269.
  40. Song OR, Lee SJ, Lee YS, Lee SC, Kim KK, Choi YL. 2008. Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA 23 isolated from cultivated soil. Brazilian Journal of Microbiology 39:151-156. https://doi.org/10.1590/S1517-83822008000100030
  41. Suri VK, Choudhary AK, Girish C, Verma TS, Gupta MK, Dutt N. 2011. Improving Phosphorus Use through Co-inoculation of Vesicular Arbuscular Mycorrhizal Fungi and Phosphate-Solubilizing Bacteria in Maize in an Acidic Alfisol. Communications in Soil Science and Plant Analysis 42:2265-2273. https://doi.org/10.1080/00103624.2011.602451
  42. Sylvia DM, Hartel PG, Fuhrmann JJ, Zuberer DA. 2005. Principles and applications of soil microbiology. Pearson prentice hall. Englewood Cliffs, NJ.
  43. Tripura CB, Sashidhar B, Podile AR. 2005. Transgenic mineral phosphate solubilizing bacteria for improved agricultural productivity. In: T. Satyanarayana and B.N. Johri (eds.) Microbial Diversity Current Perspectives and Potential Applications. pp. 375-392. I. K. International Pvt. Ltd. pp. 375-392.
  44. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  45. Vikram A, Hamzehzarghani H. 2008. Effect of phosphate solubilizing bacteria on nodulation and growth parameters of green gram (Vigna radiate L. Wilchek). Research Journal of Microbiology 3:62-72. https://doi.org/10.3923/jm.2008.62.72
  46. Walpola BC, Yoon MH. 2013. Phosphate solubilizing bacteria: Isolation and assess their effect on growth promotion and phosphorous uptake of green gram plants (Vigna radiata [L.] R. Wilczek). Chilean Journal of agricultural research 73:275-281. https://doi.org/10.4067/S0718-58392013000300010
  47. Walpola BC, Kong WC, Yoon MH. 2013. Solubilization of Inorganic Phosphates and Plant Growth Promotion by Pantoea Strains. Korean Journal of Soil Science and Fertilizer 46:494-501. https://doi.org/10.7745/KJSSF.2013.46.6.494
  48. Yu X, Liu X, Zhu TH, Liu GH, Mao C. 2011. Isolation and characterization of phosphate solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Biology and Fertility of Soils 47:437-446. https://doi.org/10.1007/s00374-011-0548-2
  49. Zaidi A, Khan MS, Amil M. 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea European Journal of Agronomy 9:15-21.

Cited by

  1. 양송이배지로부터 분리한 Pantoea rodasil 와 Burkholderia stabilis의 인산가용화능 비교 vol.16, pp.1, 2016, https://doi.org/10.14480/jm.2018.16.1.31