Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Molecular Detection of Spirometra decipiens in the United States

  • Jeon, Hyeong-Kyu (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Park, Hansol (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Lee, Dongmin (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Choe, Seongjun (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Eom, Keeseon S. (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine)
  • Received : 2016.04.04
  • Accepted : 2016.06.08
  • Published : 2016.08.31
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Abstract

The genus Spirometra belongs to the family Diphyllobothriidae and order Pseudophyllidea, and includes intestinal parasites of cats and dogs. In this study, a plerocercoid labeled as Spirometra mansonoides from the USA was examined for species identification and phylogenetic analysis using 2 complete mitochondrial genes, cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit 3 (nad3). The cox1 sequences (1,566 bp) of the plerocercoid specimen (USA) showed 99.2% similarity to the reference sequences of the plerocercoid of Korean Spirometra decipiens (GenBank no. KJ599679), and 99.1% similarity in regard to nad3 (346 bp). Phylogenetic tree topologies generated using 4 analytical methods were identical and showed high confidence levels with bootstrap values of 1.00, 100%, 100%, and 100% for Bayesian inference (BI), maximum-likelihood (ML), neighbor-joining (NJ), and maximum parsimony (MP) methods, respectively. Representatives of Diphyllobothrium and Spirometra species formed a monophyletic group, and the sister-genera status between these species was well supported. Trapezoic proglottids in the posterior 1/5 region of an adult worm obtained from an experimentally infected cat were morphologically examined. The outer uterine loop of the uterus coiling characteristically consisted of 2 complete turns. The results clearly indicated that the examined Spirometra specimen from the USA matched to S. decipiens very well, and indicated possible presence of the life cycle of this species in this region.

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References

  1. Faust EC, Campbell HE, Kellogg CR. Morphological and biological studies on the species of Diphyllobothrium in China. Am J Hyg 1929; 9: 560-583.
  2. Mueller JF. A repartition of the genus Diphyllobothrium. J Parasitol 1937; 23: 308-310. https://doi.org/10.2307/3272427
  3. Mueller JF. New host records for Diphyllobothrium mansonoides Mueller 1935. J Parasitol 1937; 23: 313-315.
  4. Wardle RA, McLeod JA. The Zoology of Tapeworms. Minneapolis, USA. University of Minnesota Press. 1952, pp 559-615.
  5. Yamaguti S. Systema helminthum. Vol. II. The cestodes of vertebrates. New York, USA. Interscience Publishers. 1959, pp 358-361.
  6. Kamo H. Guide to identification of diphyllobothriid cestodes. Gendai Kikaku, Tokyo, Japan. 1999, pp 1-146 (in Japanese).
  7. Iwata S. Experimental and morphological studies of Manson's tapeworm, Diphyllobothrium erinacei, Rudolphi. Special reference with its scientific name and relationship with Sparganum proliferum, Ijima. Progress Med Parasitol Jpn 1972; 4: 536-590.
  8. Lee SU, Huh S, Phares CK. Genetic comparison between Spirometra erinacei and S. mansonoides using PCR-RFLP analyses. Korean J Parasitol 1997; 35: 277-282. https://doi.org/10.3347/kjp.1997.35.4.277
  9. Jeon HK, Park HS, Lee DM, Choe SJ, Kim KH, Huh S, Sohn WM, Chai JY, Eom KS. Human infections with Spirometra decipiens plerocercoids identified by morphologic and genetic analyses in Korea. Korean J Parasitol 2015; 53: 299-305. https://doi.org/10.3347/kjp.2015.53.3.299
  10. Eom KS, Park HS, Lee DM, Choe SJ, Kim KH, Jeon HK. Mitochondrial genome sequences of Spirometra erinaceieuropaei and S. decipiens (Cestoidea: Diphyllobothriidae). Korean J Parasitol 2015; 53: 455-463. https://doi.org/10.3347/kjp.2015.53.4.455
  11. Swofford DL. Paup*: phylogenetic analysis using parsimony (*and other methods). Version 4. Sunderland, Massachusettes, USA. Sinauer Associates. 2003.
  12. Lanfear R, Calcott B, Hos YW, Guindon S. Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Mol Biol Evol 2012; 29: 1695-1701. https://doi.org/10.1093/molbev/mss020
  13. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP. MrBayes v3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 2012; 61: 539-542. https://doi.org/10.1093/sysbio/sys029
  14. Chandler AC. The helminthic parasites of cats in Calcutta and the relation of cats to human helminthic infections. Indian J Med Res 1925; 13: 213-227.

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