Journal of radiological science and technology (대한방사선기술학회지:방사선기술과학)

Korean Society of Radiological Science (대한방사선과학회)
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The Effects of Cynanchi wilfordii Radix Ethanol Extracts upon Irradiated Rat's Blood and Organ

백하수오 에탄올추출물이 방사선조사에 따른 흰쥐의 혈구 및 장기에 미치는 영향

  • Kim, Jang-Oh (Department of Emergency Management.Inje University) ;
  • Choi, Jun-Hyeok (Radiation Safety Research Center, Inje University) ;
  • Shin, Ji-Hye (Department of Emergency Management.Inje University) ;
  • Jung, Do-Young (Department of Radiation Oncology, Dongnam Institute of Radiological & Medical Sciences Cancer Center) ;
  • Min, Byung-In (Department of Nuclear Applied Engineering, Inje University)
  • 김장오 (인제대학교 재난관리학과) ;
  • 최준혁 (인제대학교 방사선방재센터) ;
  • 신지혜 (인제대학교 재난관리학과) ;
  • 정도영 (동남권원자력의학원 방사선종양학과) ;
  • 민병인 (인제대학교 원자력응용공학부)
  • Received : 2016.07.31
  • Accepted : 2016.09.21
  • Published : 2016.09.30
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Abstract

The development of radioprotector is being actively conducted in order to reduce the damage from over radiation exposure at radiation accident or radiation therapy. So this study was confirmed for radiation protective effects using the Cynanchi wilfordii Radix that has been known to be effective for antioxidant activity, anti-cancer, immune enhancing effects. The method of this study was administered orally Cynanchi wilfordii Radix ethanol extracts to Sprague Dawley Rat(SD Rat) for 14 days once a day, while measuring changed blood cell, spleen index, liver and uterus tissue along the change in time of 1, 4, 7 and 21 days after X-ray beam of 7 Gy irradiation. As the result of the experiment, the experimental group's rats which are administered with Cynanchi Wilfordii Radix ethanol extracts showed a rapid recovery in white blood cell count(p < 0.05) and spleen index(p < 0.05). In addition, condensation of nuclei, cytoplasmic swelling, and inflammatory cell infiltration in experimental group's liver cell was decreased more than in irradiation group's component. Further, experimental group's Uterine gland decreased the apoptosis more than irradiation group's components did. It is expected that Cynanchi Wilfordii Radix extracts will be useful as a new radioprotector. With above in mind, this paper may provide appropriate implications with the field of emergency management such as radiation accident.

방사선 사고 및 방사선 치료 등 방사선에 의한 과피폭의 피해를 줄이기 위해 방사선방호제 개발연구는 활발히 진행되고 있다. 이에 화학적 합성물이 아닌 항산화, 항암, 면역 증강에 효과적인 것으로 알려진 백하수오를 이용하여 방사선방호효과를 확인하였다. 백하수오 에탄올추출물을 Sprague Dawley Rat (SD Rat)에 14일간 1일 1회 경구 투여하고, 7 Gy X-ray를 조사한 후 1일, 4일, 7일, 21일의 시간 변화에 따른 혈구성분, 비장 지수의 변화 및 간과 자궁의 조직변화를 관찰하였다. 실험결과 백하수오 에탄올추출물을 섭취한 실험군의 백혈구 수치(p < 0.05)와 비장 지수(p < 0.05)가 대조군보다 회복이 빠른 것을 확인하였다. 간 조직에서는 핵의 응축, 세포질의 팽창, 염증세포의 침윤이 감소하였으며, 자궁샘 조직은 세포고사가 감소한 것을 확인하였다. 위의 결과를 토대로 백하수오 에탄올추출물은 방사선 조사에 따른 혈구 및 장기의 피해를 줄일 수 있는 새로운 방사선방호제로써 유용할 것으로 기대되며, 방사선 사고와 같은 비상관리 분야에 적절한 시사점을 제공할 수 있다.

Keywords

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