Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Optimization of the re-extraction process of ethanol from catechin components from Korean green tea extract

한국산 녹차추출물 catechin 성분의 ethanol 재추출 공정 최적화

  • Jo, In-Hee (Research and Development Center, SAMKYUNG COSTECH Co., Ltd.) ;
  • Kim, Myung-Kyoo (Research and Development Center, SAMKYUNG COSTECH Co., Ltd.) ;
  • Jang, Suh-Yoon (Research and Development Center, SAMKYUNG COSTECH Co., Ltd.) ;
  • Kim, Na-Ra (Research and Development Center, SAMKYUNG COSTECH Co., Ltd.) ;
  • Lee, Won-Young (Department of Food Science and Technology, Kyungpook National University)
  • 조인희 ((주)삼경코스텍 연구개발) ;
  • 김명규 ((주)삼경코스텍 연구개발) ;
  • 장서윤 ((주)삼경코스텍 연구개발) ;
  • 김나라 ((주)삼경코스텍 연구개발) ;
  • 이원영 (경북대학교 식품공학부)
  • Received : 2016.06.02
  • Accepted : 2016.07.20
  • Published : 2016.10.30
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Abstract

The purpose of this study was to determine the optimum re-extraction conditions of ethanol from catechin in Korean green tea (Camellia sinensis L.) using response surface methodology (RSM). The experiments were carried out according to a five level and two variable central composite design (CCD). The two independent variables were solvent ratio to sample content (1, 4, 7, 10, 13 mL/g) and extraction temperature (-20, -10, 0, 10, $20^{\circ}C$) on the dependent variables including yield, epigallocatechin (EGC), epicathchin (EC), epigallocatechingallate (EGCG), epicatechingallate (ECG), total catechin and caffeine. ANOVA results showed that Coefficients of determination ($R^2$) of estimated models for dependent variables were ranged from 0.9054~0.9778, while $R^2$ of caffeine were estimated 0.8770. The optimum ranges for the maximized extraction including yield, EGC, EC, EGCG, ECG, caffeine and total catechin were 4.5~7.5 mL/g in ratio of ethanol to sample and $-8{\sim}8^{\circ}C$ in extraction temperature. The actual values of yield, EGC, EC, EGCG, ECG, caffeine and total catechin, respectively, at the optimized conditions were 35.02%, 13.31%, 3.978%, 19.11%, 4.29%, 5.30% and 40.68%.

녹차 추출물의 에탄올을 이용한 카테킨 성분의 고순도 및 고효율 추출을 위한 추출조건을 반응표면분석법을 사용하여 최적화하였다. 고형분 수율은 에탄올 사용 비율 및 온도 조건 모두 유의적으로 영향을 받았다(p<0.001). 녹차의 카테킨 성분 함량 및 총 카테킨 함량 모두 1%이내의 유의성이 인정되었고 온도보다 에탄올 사용 비율 조건이 추출결과에 더 영향을 미친다. 카페인 함량은 에탄올 사용비율에 유의적으로 영향을 받았다. 반응표면 분석 결과 카페인을 제외한 종속변수의 $R^2$값은 0.90 이상으로 1% 이내의 유의성이 인정되었다. 예측된 조건은 에탄올 사용 비율이 6 mL/g, 온도가 $0^{\circ}C$에서 고형분 수율 33.01%, EGC 12.56%, EC 3.78%, EGCG 22.43%, ECG 5.39%로 총 카테킨 함량은 40.17%이며 카페인 함량은 5.87%로 예측되었다. 예측된 조건에서 실제 추출시 고형분 수율은 35.02%, EGC 13.31%, EC 3.97%, EGCG 19.11%, ECG 4.29%로 총 카테킨 함량은 40.68%이며 카페인 함량은 5.30%로 분석되었다.

Keywords

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