DOI QR코드

DOI QR Code

Characteristics of iron powder formulation produced from porcine blood by enzymatic treatment

효소 처리한 돈혈 활용 철분분말제제 특성

  • Received : 2016.06.01
  • Accepted : 2016.06.21
  • Published : 2016.10.30

Abstract

In this study, enzyme (thermoase) hydrolysis was applied to the porcine blood order to increase the iron content and solubility. It was confirmed that content of iron was increase up to 158.11 mg/100 g porcine powders after 0.2% thermoase treatment at $60^{\circ}C$ during 4 hr. The solubility of porcine blood powders was higher than other enzyme (various protease), temperature, reaction time. This optimized conditions were also worked to the in vitro iron bioavailability rate increasement, the bioavailability of hydolyzed porcine powders was 3-fold higher than that of an iron supplement on the market. These results indicate the possibility of porcine blood powder in iron supplements market as natural material. Also utilizing of reduced porcine blood will be possible to improve environmental issues.

본 연구에서는 도축장에서 폐수 처리되는 돈혈을 활용하여 단백질 분해 효소 처리를 하여 철분함량 및 용해도가 높은 철분분말을 제조하고자 하였다. 철분함량을 높이기 위하여 효소처리 및 혈액 분리 정도에 따른 돈혈 분말의 품질특성을 분석하였고, 그 결과 수급 받은 돈혈의 전혈에 Thermoase 0.2%를 첨가한 후 $60^{\circ}C$에서 4시간 처리하여 여과, 열풍건조처리를 하였을 때 철분함량이 110.00 mg/100 g 으로 가장 높은 것으로 확인되었으며, 재수화성 역시 높은 것을 확인하였다. 또 철분 보조제로써 활용 가능 여부를 확인하기 위하여 최종조건에 따라 제조 된 돈혈분말의 생체이용률을 측정하였으며, 대표적인 시중 판매 제품 보다 2.9배 높은 것으로 확인 되었다. 따라서 영양학적 가치가 있는 돈혈을 활용하여 철분분말을 제조한다면, 수입에 의존하고 있는 철분시장에 국내산 천연재료 공급이 가능할 뿐만 아니라 이의 폐기처리로 인한 비용처리 및 환경오염을 줄일 수 있을 것으로 기대된다.

Keywords

References

  1. Kye SH, Paik HY (1993) Iron nutriture and related dietary factors in apparently healthy young Korean women (2): analysis of iron in major food items and assessment of intake and availability of dietary iron. Korean J Nutr, 26, 703-714
  2. Kye SH, Paik HY (1993) Iron nutriture and related dietary factors in apparently healthy young Korean women (1): comparison and evaluation of blood bichemical indices for assessment of iron nutritional status. Korean J Nutr, 26, 692-702
  3. Bezkorovainy A (1989) Biochemistry of nonheme iron in man. II. Absorption of iron. Clin Physiol Biochem, 7, 53-69
  4. Kang IK, Chae HJ, In MJ, Oh NS (2003) A maufacturing processes of enriched heme iron. Food Industry and Natrition, 8, 43-50
  5. Carpenter CE, Mahoney AW (1992) Contributions of heme and nonheme iron to human nutrition. Crit Rev Food Sci Nutr, 31, 333-367 https://doi.org/10.1080/10408399209527576
  6. Shim YH, Ha WH, Cho JH, Kim DH, Kim YL, Kim HJ (2002) Identification of ferritin using immunodiffusion methods. Kor J Pharmacogn, 33, 257-261
  7. Lebrun F, Bazus A, Dhulster P, Guillochon D (1998) Solubility of heme in heme-iron enriched bovine hemoglobin hydrolysates. J Agric Food Chem, 46, 5017-5025 https://doi.org/10.1021/jf9805698
  8. Cho HJ, Lee HS, Jung EY, Park SY, Lim WT, Lee JY, Yeon SH, Lee JC, Shu HJ (2010) Manufacturing of iron binding peptide using sericin hydrolysate and its bioavailability in iron deficient rat. J Korean Soc Food Sci Nutr, 39, 1446-1451 https://doi.org/10.3746/jkfn.2010.39.10.1446
  9. Kim GE (2014) Protein degradation in waste blood for liquid fertilizer production. J Korean Soc Vrban Enriron, 14, 127-134
  10. Ockeman HW, Hansen CL (1988) Blood utilization. In: Animal By-product processing& Utilization, CRC press LLC, Washington, DC, p. 325-353
  11. Park GH (1997) Optimum drying condition for slaughter porcine blood and its utilization as broiler diets. Korea J Poult Sci, 24, 59-66
  12. Hyun CK, Shin HG (1999) Production of angiotensin I converting enzyme inhibitory peptides from bovine blood plasma proteins. Korean J Biotechnol Bioeng, 14, 600-605
  13. Park JY, Kim MY, Jeong YJ (2016) Changes in physicochemical characteristics of porcine blood under various conditions of enzyme hydrolysis. Korean J. Food Preserv, 23, 413-421 https://doi.org/10.11002/kjfp.2016.23.3.413
  14. Choi JP (2013) Animal byproduct (blood) current status & usage. Food Science and Industry of Animal Resources, 2, 2-7
  15. Hugli TE, Moore S (1972) Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates. J Biol Chem, 247, 2828-2834
  16. Miller DD, Schricker BR, Rasmussen RR, Campen DV (1981) An in vitro method for esimation of iron availability from meals. Am J Clin Nutr, 34, 2248-2256 https://doi.org/10.1093/ajcn/34.10.2248
  17. Kim JH, Yoo CJ, Sin KA, Jang SY, Park NY, Jeong YJ (2011) Changes in properties of deer antler by proteolysis and extraction conditions. J Korean Soc Food Sci Nutr, 40, 89-93 https://doi.org/10.3746/jkfn.2011.40.1.089
  18. Noh JE, Yoon SR, Lim AK, Kim HJ, Huh D, Kim DI (2012) A study on the yield of functional components of citrus peel extracts using optimized hot water extraction and enzymatic hydrolysis. Korean J Food Cookery Sci, 28, 51-55 https://doi.org/10.9724/kfcs.2012.28.1.051
  19. Ait-Oukhatar N, Peres JM, Bouhallab S, Neuville D, Bureau F, Bouvard G, Arhan P, Bougle D (2002) Bioavailability of caseinophosphopeptide-bound iron. J Lab Clin Med, 140, 290-294 https://doi.org/10.1067/mlc.2002.128146
  20. Kim SB, Seo IS, Khan MA, Ki KS, Lee WS, Lee HJ, Shin HS, Kim HS (2007) Enzymatic hydrolysis of heated whey: iron-binding ability of peptides and antigenic protein fractions. J Dairy Sci, 90, 4033-4042 https://doi.org/10.3168/jds.2007-0228
  21. Kim SB Seo IS, Khan MA, Nam MS, Kim HS(2007) Separation of iron-binding protein from whey through enzymatic hydrolysis. Int Dairy J, 17, 625-631 https://doi.org/10.1016/j.idairyj.2006.09.001
  22. Seth A, Mahoney RR (2000) Binding of iron by chicken muscle protein digests: the size of the iron-binding peptides. J Sci Food Agric, 80, 1595-1600 https://doi.org/10.1002/1097-0010(20000901)80:11<1595::AID-JSFA684>3.0.CO;2-Y

Cited by

  1. Development of livestock feed additives using porcine blood cells vol.105, pp.3, 2021, https://doi.org/10.1111/jpn.13498