Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison of Linarin Content and Biological Activity in Ethanol Extraction of Chrysanthemum zawadskii

구절초의 추출조건에 따른 Linarin 함량과 In Vitro 생리활성 변화

  • Kim, Yang-Ji (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.) ;
  • Kim, Seong-Eun (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.) ;
  • Lee, Hak Sung (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.) ;
  • Hong, Su-Young (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.) ;
  • Kim, Sung-Eun (Functional Foods Institute, KolmarBNH Co., Ltd.) ;
  • Kim, Young Jun (Department of Food and Biotechnology, Korea University) ;
  • Lee, Jin Hyup (Department of Food and Biotechnology, Korea University) ;
  • Park, Sung Jin (Department of Food and Biotechnology, Korea University) ;
  • Kim, Jun Ho (Department of Food and Biotechnology, Korea University) ;
  • Park, Youn-Je (Department of Food Science and Technology, Kongju National University) ;
  • Kim, Hyun-Kyu (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.)
  • 김양지 (콜마비앤에이치(주) 신소재식품연구소) ;
  • 김성은 (콜마비앤에이치(주) 신소재식품연구소) ;
  • 이학성 (콜마비앤에이치(주) 신소재식품연구소) ;
  • 홍수영 (콜마비앤에이치(주) 신소재식품연구소) ;
  • 김성은 (콜마비앤에이치(주) 기능식품연구소) ;
  • 김영준 (고려대학교 식품생명공학과) ;
  • 이진협 (고려대학교 식품생명공학과) ;
  • 박성진 (고려대학교 식품생명공학과) ;
  • 김준호 (고려대학교 식품생명공학과) ;
  • 박윤제 (공주대학교 식품공학과) ;
  • 김현규 (콜마비앤에이치(주) 신소재식품연구소)
  • Received : 2016.06.14
  • Accepted : 2016.08.04
  • Published : 2016.10.31
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Abstract

This study was designed to determine optimal extraction conditions of Chrysanthemum zawadskii (CZ) with high yield of bioactive compounds (linarin, polyphenols, and flavonoids) and improved in vitro activities. Ethanol extracts were examined at different concentrations (0, 30, 50, 70, and 95%) and temperatures (50 and $70^{\circ}C$). The recommended extraction conditions were 50% ethanol at $70^{\circ}C$ for 4 h, which gave the highest yield of linarin, polyphenols, and flavonoids. This extract appeared to have the greatest ABTS and DPPH radical scavenging activities (87.81% and 51.42% at $500{\mu}g/mL$, respectively) and ${\alpha}$-glucosidase (74.59%) and pancreatic lipase inhibitory activities (90.44%). As contents of linarin increased, antioxidative activity, inhibitory effects of carbohydrates and lipid absorption of CZ extracts increased as well. From these results, CZ extracts can be used in functional food industries.

본 연구에서는 구절초를 대상으로 물과 에탄올을 이용하여 확보한 각각의 추출물에 대하여 추출 수율, 폴리페놀 함량, 플라보노이드 함량, linarin 함량, ABTS 라디칼 소거능과 DPPH 라디칼 소거능, ${\alpha}$-glucosidase 저해 활성, lipase 저해 활성을 측정하였다. 구절초는 $70^{\circ}C$, 50% 주정 추출조건에서 추출 수율과 총플라보노이드 함량, linarin 함량, ABTS 라디칼 소거능과 DPPH 라디칼 소거능, ${\alpha}$-glucosidase 저해 활성, lipase 저해 활성이 가장 높았다. 또한, linarin 함량이 구절초 추출물의 생리활성과 상관관계가 있음을 확인하였다. 구절초 추출물은 linarin 함량이 증가함에 따라 항산화, 탄수화물 및 지방 흡수 지연 활성을 가지는 것으로 생각한다. 본 연구를 통하여 구절초의 추출조건에 따른 생리활성을 확인함으로써 향후 구절초를 이용한 건강기능식품의 소재 등으로 이용 가능할 것으로 생각한다.

Keywords

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