Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Association study analysis of CD9 as candidate gene for Duroc pig sperm motility and kinematic characteristics

두록 정자 운동학적 특성과 후보유전자 CD9 유전자와의 연관성 분석

  • Jeong, Yong-dae (National Institute of Animal Science, Rural Development Adminstration) ;
  • Jeong, Jin-Young (National Institute of Animal Science, Rural Development Adminstration) ;
  • Kim, Ki-Hyun (National Institute of Animal Science, Rural Development Adminstration) ;
  • Cho, Eun-Seok (National Institute of Animal Science, Rural Development Adminstration) ;
  • Yu, Dong-Jo (National Institute of Animal Science, Rural Development Adminstration) ;
  • Choi, Jung-Woo (College of Animal Life Science, Kangwon National University) ;
  • Jang, Hyun-Jun (College of Pharmacy, Dankook University) ;
  • Park, Sungk-won (Department of Food Science & Technology, Sejong University) ;
  • Sa, Soo-Jin (National Institute of Animal Science, Rural Development Adminstration) ;
  • Woo, Jae-Seok (National Institute of Animal Science, Rural Development Adminstration)
  • 정용대 (농촌진흥청 국립축산과학원) ;
  • 정진영 (농촌진흥청 국립축산과학원) ;
  • 김기현 (농촌진흥청 국립축산과학원) ;
  • 조은석 (농촌진흥청 국립축산과학원) ;
  • 유동조 (농촌진흥청 국립축산과학원) ;
  • 최정우 (강원대학교 동물생명과학대학) ;
  • 장현준 (단국대학교 약학대학) ;
  • 박성권 (세종대학교 생명과학대학 식품생명공학과) ;
  • 사수진 (농촌진흥청 국립축산과학원) ;
  • 우제석 (농촌진흥청 국립축산과학원)
  • Received : 2016.09.09
  • Accepted : 2016.09.29
  • Published : 2016.09.30
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Abstract

Cluster-of-differentiation antigen 9 (CD9) gene expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as a candidate gene to investigate Duroc boar semen motility and kinematic characteristics. This study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 96 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT, $82.27{\pm}5.58$), Curvilinear velocity(VCL, $68.37{\pm}14.58$), Straight-line velocity(VSL, $29.06{\pm}6.58$), the ratio between VSL and VCL(LIN, $47.36{\pm}8.42$), Amplitude of Lateral Head displacement(ALH, $2.88{\pm}0.70$)] were used in present study. A single nucleotide polymorphism (g.358A>T) in intron 6 was associated with MOT, VCL, VAP and ALH in Duroc population (p<0.05). Therefore, we suggest that the porcine CD9 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the CD9 in spermatogenesis within the reproductive tracts, and will shed light on CD9 as a candidate gene in the selection of good sperm quality boars.

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References

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