DOI QR코드

DOI QR Code

스트레스 유발 마우스모델에서 뇌염증 및 신경행동 장애 변화

Neuro-inflammation induced by restraint stress causes impairs neurobehavior in mice

  • 오태우 (한국한의학연구원 한의기술응용센터) ;
  • 도현주 (한국한의학연구원 한의기술응용센터) ;
  • 김광연 (한국한의학연구원 한의기술응용센터) ;
  • 김영우 (대구한의대학교 한의과대학) ;
  • 이병욱 (동국대학교 한의과대학) ;
  • 마진열 (한국한의학연구원 한의기술응용센터) ;
  • 박광일 (한국한의학연구원 한의기술응용센터)
  • Oh, Tae woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Do, Hyun Ju (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Kwang-Youn (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Young Woo (College of Oriental Medicine Deagu Haany University) ;
  • Lee, Byung Wook (College of Oriental Medicine Dongguk University) ;
  • Ma, Jin Yeul (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Park, Kwang Il (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 투고 : 2017.11.08
  • 심사 : 2017.11.21
  • 발행 : 2017.11.30

초록

Background : Behavioral stress has been suggested as one of the significant factors that is able to disrupt physiological systems and cause depression as well as changes in various body systems. The stressful events can alter cognition, learning, memory and emotional responses, resulting in mental disorders such as depression and anxiety. Results : We used a restraint stress model to evaluate the alteration of behavior and stress-related blood parameter. The animals were randomly divided into two groups of five animals each group. Furthermore, we assessed the change of body weight to evaluate the locomotor activity as well as status of emotional and anxiety in mice. After 7 days of restraint stress, the body weight had significantly decreased in the restraint stress group compared with the control group. We also observed stress-associated behavioral alterations, as there was a significant decrease in open field and forced swim test, whereas the immobilization time was significantly increased in the stress group compared to the control group. We observed the morphological changes of neuronal death and microglia by immunohistochemistry and western blot. In our study restraint stress did not cause change in neuronal cell density in the frontal cortex and CA1 hippocampus region, but there was a trend for an increased COX-2 and iNOS protein expression and microglia (CD11b) in brain, which is restraint stress. Conclusion : Our study, there were significant alterations observed in the behavioral studies. We found that mice undergoing restraint stress changed behavior, confirming the increased expression of inflammatory factors in the brain.

키워드

참고문헌

  1. Kim JJ, Diamond DM. The stressed hippocampus, synaptic plasticity and lost memories. Nat Rev Neurosci 2002, 3:453-462.
  2. Lecci A, Borsini F, Volterra G, Meli A. Pharmacological validation of a novel animal model of anticipatory anxiety in mice.Psychopharmacology (Berl) 1990, 101:255-261. https://doi.org/10.1007/BF02244136
  3. Kim KS, Kwon HJ, Baek IS, Han PL. Repeated Short-term (2hx14d) Emotional Stress Induces Lasting Depression-like Behavior in Mice.Exp Neurobiol 2012, 21:16-22. https://doi.org/10.5607/en.2012.21.1.16
  4. Johnson EO, Kamilaris TC, Chrousos GP, Gold PW. Mechanisms of stress: a dynamic overview of hormonal and behavioral homeostasis.Neurosci Biobehav Rev 1992, 16:115-130. https://doi.org/10.1016/S0149-7634(05)80175-7
  5. Buynitsky T, Mostofsky DI. Restraint stress in biobehavioral research: Recent developments.Neurosci Biobehav Rev 2009, 33:1089-1098. https://doi.org/10.1016/j.neubiorev.2009.05.004
  6. Lau C, Simpson C. Animal models for the study of the effect of prolonged stress on lactation in rats.Physiol Behav 2004, 82:193-197. https://doi.org/10.1016/j.physbeh.2004.05.004
  7. Reid MB. Nitric oxide, reactive oxygen species, and skeletal muscle contraction.Med Sci Sports Exerc 2001, 33:371-376. https://doi.org/10.1097/00005768-200103000-00006
  8. Crane JW, French KR, Buller KM. Patterns of neuronal activation in the rat brain and spinal cord in response to increasing durations of restraint stress.Stress 2005, 8:199-211. https://doi.org/10.1080/10253890500333817
  9. McEwen BS. The neurobiology of stress: from serendipity to clinical relevance.Brain Res 2000, 886:172-189. https://doi.org/10.1016/S0006-8993(00)02950-4
  10. Gagliano H, Fuentes S, Nadal R, Armario A. Previous exposure to immobilisation and repeated exposure to a novel environment demonstrate a marked dissociation between behavioral and pituitary-adrenal responses. Behav Brain Res 2008, 187:239-245. https://doi.org/10.1016/j.bbr.2007.09.006
  11. Strohle A, Hofler M, Pfister H, Muller AG, Hoyer J, Wittchen HU, Lieb R. Physical activity and prevalence and incidence of mental disorders in adolescents and young adults.Psychol Med 2007, 37:1657-1666.
  12. Jacobson L. Hypothalamic-pituitary-adrenocortical axis regulation.Endocrinol Metab Clin North Am 2005, 34:271-292, vii. https://doi.org/10.1016/j.ecl.2005.01.003
  13. Charmandari E, Tsigos C, Chrousos G. Endocrinology of the stress response.Annu Rev Physiol 2005, 67:259-284. https://doi.org/10.1146/annurev.physiol.67.040403.120816
  14. Romero LM. Seasonal changes in plasma glucocorticoid concentrations in free-living vertebrates.Gen Comp Endocrinol 2002, 128:1-24. https://doi.org/10.1016/S0016-6480(02)00064-3
  15. Caso JR, Moro MA, Lorenzo P, Lizasoain I, Leza JC. Involvement of IL-1beta in acute stress-induced worsening of cerebral ischaemia in rats.Eur Neuropsychopharmacol 2007, 17:600-607. https://doi.org/10.1016/j.euroneuro.2007.02.009
  16. Madrigal JL, Moro MA, Lizasoain I, Lorenzo P, Castrillo A, Bosca L, Leza JC. Inducible nitric oxide synthase expression in brain cortex after acute restraint stress is regulated by nuclear factor kappaB-mediated mechanisms.J Neurochem 2001, 76:532-538. https://doi.org/10.1046/j.1471-4159.2001.00108.x
  17. Garcia-Bueno B, Madrigal JL, Lizasoain I, Moro MA, Lorenzo P, Leza JC. The anti-inflammatory prostaglandin 15d-PGJ2 decreases oxidative/nitrosative mediators in brain after acute stress in rats.Psychopharmacology (Berl) 2005, 180:513-522. https://doi.org/10.1007/s00213-005-2195-5
  18. Madrigal JL, Garcia-Bueno B, Caso JR, Perez- Nievas BG, Leza JC. Stress-induced oxidative changes in brain.CNS Neurol Disord Drug Targets 2006, 5:561-568. https://doi.org/10.2174/187152706778559327
  19. Bardin L, Malfetes N, Newman-Tancredi A, Depoortere R. Chronic restraint stress induces mechanical and cold allodynia, and enhances inflammatory pain in rat: Relevance to human stress-associated painful pathologies.Behav Brain Res 2009, 205:360-366. https://doi.org/10.1016/j.bbr.2009.07.005
  20. Takuma K, Hoshina Y, Arai S, Himeno Y, Matsuo A, Funatsu Y, Kitahara Y, Ibi D, Hayase M, Kamei H, et al. Ginkgo biloba extract EGb 761 attenuates hippocampal neuronal loss and cognitive dysfunction resulting from chronic restraint stress in ovariectomized rats.Neuroscience 2007, 149:256-262. https://doi.org/10.1016/j.neuroscience.2007.07.042
  21. Monteggia LM, Luikart B, Barrot M, Theobold D, Malkovska I, Nef S, Parada LF, Nestler EJ. Brain-derived neurotrophic factor conditional knockouts show gender differences in depressionrelated behaviors.Biol Psychiatry 2007, 61:187-197. https://doi.org/10.1016/j.biopsych.2006.03.021
  22. Abidin I, Yargicoglu P, Agar A, Gumuslu S, Aydin S, Ozturk O, Sahin E. The effect of chronic restraint stress on spatial learning and memory: Relation to oxidant stress.International Journal of Neuroscience 2004, 114:683-699. https://doi.org/10.1080/00207450490430543
  23. Yalcin I, Bohren Y, Waltisperger E, Sage-Ciocca D, Yin JC, Freund-Mercier MJ, Barrot M. A time-dependent history of mood disorders in a murine model of neuropathic pain.Biol Psychiatry 2011, 70:946-953. https://doi.org/10.1016/j.biopsych.2011.07.017
  24. Urban R, Scherrer G, Goulding EH, Tecott LH, Basbaum AI. Behavioral indices of ongoing pain are largely unchanged in male mice with tissue or nerve injury-induced mechanical hypersensitivity.Pain 2011, 152:990-1000. https://doi.org/10.1016/j.pain.2010.12.003
  25. Parent AJ, Beaudet N, Beaudry H, Bergeron J, Berube P, Drolet G, Sarret P, Gendron L. Increased anxiety-like behaviors in rats experiencing chronic inflammatory pain.Behav Brain Res 2012, 229:160-167. https://doi.org/10.1016/j.bbr.2012.01.001
  26. Hummel M, Lu P, Cummons TA, Whiteside GT. The persistence of a long-term negative affective state following the induction of either acute or chronic pain.Pain 2008, 140:436-445. https://doi.org/10.1016/j.pain.2008.09.020
  27. Leite-Almeida H, Cerqueira JJ, Wei H, Ribeiro-Costa N, Anjos-Martins H, Sousa N, Pertovaara A, Almeida A. Differential effects of left/right neuropathy on rats' anxiety and cognitive behavior.Pain 2012, 153:2218-2225. https://doi.org/10.1016/j.pain.2012.07.007
  28. Shoji H, Takao K, Hattori S, Miyakawa T. Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age.Mol Brain 2016, 9:11. https://doi.org/10.1186/s13041-016-0191-9
  29. Bhatnagar S, Vining C, Iyer V, Kinni V. Changes in hypothalamic-pituitary-adrenal function, body temperature, body weight and food intake with repeated social stress exposure in rats.J Neuroendocrinol 2006, 18:13-24. https://doi.org/10.1111/j.1365-2826.2005.01375.x
  30. Harris RB, Zhou J, Youngblood BD, Rybkin, II, Smagin GN, Ryan DH. Effect of repeated stress on body weight and body composition of rats fed low- and high-fat diets.Am J Physiol 1998, 275:R1928-1938.
  31. Harris RB, Palmondon J, Leshin S, Flatt WP, Richard D. Chronic disruption of body weight but not of stress peptides or receptors in rats exposed to repeated restraint stress.Horm Behav 2006, 49:615-625. https://doi.org/10.1016/j.yhbeh.2005.12.001
  32. Esch T, Stefano GB, Fricchione GL, Benson H. Stress in cardiovascular diseases.Med Sci Monit 2002, 8:RA93-RA101.
  33. He RR, Yao XS, Li HY, Dai Y, Duan YH, Li YF, Kurihara H. The anti-stress effects of Sarcandra glabra extract on restraint-evoked immunocompromise.Biol Pharm Bull 2009, 32:247-252. https://doi.org/10.1248/bpb.32.247
  34. Chen WW, He RR, Li YF, Li SB, Tsoi B, Kurihara H. Pharmacological studies on the anxiolytic effect of standardized Schisandra lignans extract on restraint-stressed mice.Phytomedicine 2011, 18:1144-1147. https://doi.org/10.1016/j.phymed.2011.06.004
  35. Gigon A, Matos AR, Laffray D, Zuily-Fodil Y, Pham-Thi AT. Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (ecotype Columbia).Ann Bot 2004, 94:345-351. https://doi.org/10.1093/aob/mch150
  36. Berlett BS, Stadtman ER. Protein oxidation in aging, disease, and oxidative stress.J Biol Chem 1997, 272:20313-20316. https://doi.org/10.1074/jbc.272.33.20313
  37. Mittler R. Oxidative stress, antioxidants and stress tolerance.Trends Plant Sci 2002, 7:405-410. https://doi.org/10.1016/S1360-1385(02)02312-9
  38. Pu HJ, Cao YF, He RR, Zhao ZL, Song JH, Jiang B, Huang T, Tang SH, Lu JM, Kurihara H. Correlation between Antistress and Hepatoprotective Effects of Schisandra Lignans Was Related with Its Antioxidative Actions in Liver Cells.Evid Based Complement Alternat Med 2012, 2012:161062.
  39. Zhai YJ, He RR, Tsoi B, Li YF, Li XD, Tsuruoka N, Abe K, Kurihara H. Protective effect of extract of chicken meat on restraint stress-induced liver damage in mice.Food Funct 2012, 3:662-667. https://doi.org/10.1039/c2fo10275g
  40. Esch T, Stefano GB, Fricchione GL, Benson H. The role of stress in neurodegenerative diseases and mental disorders.Neuro Endocrinol Lett 2002, 23:199-208.
  41. Yamagata K, Andreasson KI, Kaufmann WE, Barnes CA, Worley PF. Expression of a mitogeninducible cyclooxygenase in brain neurons: regulation by synaptic activity and glucocorticoids. Neuron 1993, 11:371-386. https://doi.org/10.1016/0896-6273(93)90192-T
  42. Munhoz CD, Garcia-Bueno B, Madrigal JL, Lepsch LB, Scavone C, Leza JC. Stress-induced neuroinflammation: mechanisms and new pharmacological targets.Braz J Med Biol Res 2008, 41:1037-1046. https://doi.org/10.1590/S0100-879X2008001200001
  43. Muqbil I, Banu N. Enhancement of pro-oxidant effect of 7,12-dimethylbenz (a) anthracene (DMBA) in rats by pre-exposure to restraint stress.Cancer Lett 2006, 240:213-220. https://doi.org/10.1016/j.canlet.2005.09.008
  44. Buijsse B, Feskens EJ, Moschandreas J, Jansen EH, Jacobs DR, Jr., Kafatos A, Kok FJ, Kromhout D. Oxidative stress, and iron and antioxidant status in elderly men: differences between the Mediterranean south (Crete) and northern Europe (Zutphen).Eur J Cardiovasc Prev Rehabil 2007, 14:495-500. https://doi.org/10.1097/HJR.0b013e3280111e41
  45. Epel ES. Psychological and metabolic stress: a recipe for accelerated cellular aging?Hormones (Athens) 2009, 8:7-22. https://doi.org/10.14310/horm.2002.1217
  46. Vasquez B, Sandoval C, Smith RL, del Sol M. Effects of early and late adverse experiences on morphological characteristics of Sprague-Dawley rat liver subjected to stress during adulthood.Int J Clin Exp Pathol 2014, 7: 4627-4635.
  47. Czech B, Neumann ID, Muller M, Reber SO, Hellerbrand C. Effect of chronic psychosocial stress on nonalcoholic steatohepatitis in mice.Int J Clin Exp Pathol 2013, 6:1585-1593.