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Change of quality properties of Doenjang according to soaking method in brine

장 담금법에 따른 된장의 품질 특성 변화

  • Choi, Bo-Young (Fermented Food Science Division, National Institute of Agricultural Science) ;
  • Gil, Na-Young (Fermented Food Science Division, National Institute of Agricultural Science) ;
  • Park, Shin-Young (Fermented Food Science Division, National Institute of Agricultural Science) ;
  • Cho, Yong-Sik (Fermented Food Science Division, National Institute of Agricultural Science) ;
  • Kim, So-Young (Fermented Food Science Division, National Institute of Agricultural Science)
  • 최보영 (국립농업과학원 발효식품과) ;
  • 길나영 (국립농업과학원 발효식품과) ;
  • 박신영 (국립농업과학원 발효식품과) ;
  • 조용식 (국립농업과학원 발효식품과) ;
  • 김소영 (국립농업과학원 발효식품과)
  • Received : 2017.10.11
  • Accepted : 2017.11.16
  • Published : 2017.11.30

Abstract

This study was carried out to examine the quality characteristics of Doenjang manufactured with or without soaking Meju in brine according to salt concentrations (8 and 12%) during fermentation for 6 months. The moisture content and salinity of Doenjang fermented for 6 months were 54.9-60.3% and 7.8-12.5%, respectively. Doenjang using soaking Meju in brine had higher pH and lower titratable acidity than that using non-soaking Meju. The reducing sugar content in all samples was increased until 2 months and then decreased regardless of soaking Meju, especially that of non-soaking 8% Doenjang was the highest. The 8% low-salt Doenjang was shown the highest amino-type nitrogen content, especially the soaking Doenjang was higher than the non-soaking Doenjang. ${\alpha}$-amylase activity of all samples during fermentation were continuously decreased from 0.91-0.94 Unit/g to 0.01-0.06 Unit/g, especially the 8% soaking Doenjang was shown the highest activity after 2 months. Total bacterial count of the soaking Doenjang at the 6 months was in range of 7.8-8.0 log CFU/g and that of the non-soaking Doenjang was in range of 7.2-7.5 log CFU/g. By taste analysis, the 8% soaking Doenjang was shown the similar taste pattern with commercial Doenjang. In conclusion, the 8% low-salt Doenjang manufactured with soaking Meju in brine was a suitable concentration in order to reduce salt intake.

염수 침지 여부를 고려한 장담금법에 따른 저염 된장의 품질특성 변화를 조사하기 위하여 염 농도를 달리하여 6개월 동안 발효시킨 후 품질특성 변화를 살펴보았다. 수분 함량에서는 염수 침지한 12% 메주 된장에서 55% 수준으로 가장 낮았으며, 나머지 된장 시료들은 대략 60% 수분 함량을 나타냈다. pH는 장 담금 초기부터 메주 된장이 메줏가루 된장에 비해 높았으며, 특히 발효기간 내 8% 메주 된장의 경우 감소폭이 적었고 이는 산도 변화에서도 담금 직후부터 발효 6개월까지 메주 된장이 메줏가루 된장보다 낮은 경향을 나타내어 상관성을 보여주었다. 환원당 함량은 1-2개월 내에 최대값을 나타내고 이후 감소하는 경향을 나타냈으나, 발효기간 내 8% 메줏가루 된장이 가장 높아, 염도 및 장 담금법 간에 상관성은 낮았다. 아미노태 질소 변화는 담금 직후에는 염 농도가 낮을 수 록 높은 값을 나타냈으며, 메주 된장에서 메줏가루 된장보다 높은 값을 보였다. 암모니아태 질소 함량은 발효초기에는 12% 메줏가루 된장에서 가장 높은 값을 나타냈으며, 메줏가루 된장이 메주 된장보다 높은 값을 보였지만, 이후 급격히 감소하여 시료 간 차이를 보이지 않았다. ${\alpha}$-Amylase 효소 활성은 모든 시료가 발효 초기에는 0.91-0.94 Unit/g 수준이었다가 이후 일정하게 감소하여 0.01-0.06 Unit/g 값을 나타내었는데, 특히 8% 메주 된장에서는 완만한 감소를 보였다. Protease 효소 활성은 발효 2주차에 메주 된장이 메줏가루 된장에 비해 높은 활성을 나타냈으나 이후 감소하여 비슷한 수준을 나타내었다. 총 균수는 발효초기부터 메주 된장에서 메줏가루 된장보다 높은 값을 나타내었고, 발효 6개월째 메주 된장이 7.8-8.0 log CFU/g로 메줏가루 된장(7.2-7.5 log CFU/g)보다 통계적으로 유의하게 높은 값을 보였다. 맛 센서를 통한 맛 분석결과, 비록 12% 시판 된장에 비해 제조한 된장이 높은 짠맛과 쓴맛, 낮은 풍미를 나타내었지만, 8% 메주 된장이 시판 된장과 전반적으로 유사한 맛 패턴을 보였다. 유리 아미노산 함량은 숙성기간 동안 장가르기 여부에 따라 메줏가루 된장에서 증가폭은 컸으나 메주 된장의 함량이 높았으며, 염도 8% 된장에서 12% 된장보다 높은 값을 보였다. 결론적으로 염수 침지 여부에 따른 된장의 품질 특성을 비교한 결과, 장담금 과정을 거친 저염 된장이 품질 특성뿐만 아니라 맛 분석결과에서 좋은 평가를 나타내었고, 아울러 메줏가루를 이용한 간편 저염 장류 제품 개발을 위한 기초 자료로 활용될 수 있을 것으로 기대된다.

Keywords

References

  1. Chae HJ, Lee HJ (1990) An analytical study on Doenjang recorded in the literature. Korean Life Sci Res, 8, 29-69
  2. National Institute of Agricultural Science (2015) Manufacturing technology of fermented food and application of agricultural industry, NIAS, ISBN: 978-89-480-3354-093520, 32-33
  3. Park KY, Hwang KM, Jung KO, Lee KB (2002) Studies of the standardization of Doenjang (Korean soybean paste) Standardization of manufacturing method of Doenjang by literatures. J Korean Soc Food Sci Nutr, 31, 343-350 https://doi.org/10.3746/jkfn.2002.31.2.343
  4. Kim DY, Kwon DJ (2014) Quality characteristics of Doenjang manufactured with soybean Koji. Korean J Food Preserv, 21, 434-441 https://doi.org/10.11002/kjfp.2014.21.3.434
  5. Park JW, Lee YJ, Yoon S (2007) Total flavonoids and phenolics in fermented soy products and their effects on antioxidant activities determined by different assays. Korean J Food Cult, 22, 353-358
  6. Jung KO, Park SY, Park KY (2006) Longer aging time increases the anticancer and antimetastatic properties of Doenjang. Nutrition, 22, 539-545 https://doi.org/10.1016/j.nut.2005.11.007
  7. Lee NR, Lee SM, Cho KS, Jeong SY, Hwang DY, Kim DS, Hong CO, Son HJ (2014) Improved production of poly-${\gamma}$-glutamic acid by Bacillus subtilis D7 isolated from Doenjang, a Korean traditional fermented food, and its antioxidant activity. Appl Biochem Biotechnol, 173, 918-932 https://doi.org/10.1007/s12010-014-0908-0
  8. Yang BK, Park JB, Ha SO, Kim KY, Kym KH, Park KY, Yun JW, Song CH (2000) Hypolipidemic effect of extracts of soybean paste containing mycelia of mushrooms in hyperlipidemic rats. Korean J Micro Biotech, 28, 228-232
  9. Cha YS, Yang JA, Back HI, Kim SR, Kim MG, Jung SJ, Somg WO, Chae SW (2012) Visceral fat and body weight are reduced in overweight adults by the supplementation of Doenjang, a fermented soybean paste. Nutr Res Pract, 6, 520-526 https://doi.org/10.4162/nrp.2012.6.6.520
  10. Jang IH, Ahn MJ, Chae HJ (2004) Manufacturing method for traditional Deonjang and screening of high fibrin clotting inhibitory samples. J Appl Biol Chem, 47, 149-153
  11. Kwak CS, Park SC, Song KY (2012) Doenjang, a fermented soybean paste, decreased visceral fat accumulation and adipocyte size in rats fed with high fat diet more effectively than nonfermented soybeans. Journal of Medicinal Food, 15, 1-9 https://doi.org/10.1089/jmf.2010.1224
  12. Kim, JH, Jia Y, Lee JG, Nam BR, Lee JH, Shin KS, Hurh BS, Choi YH, Lee SJ (2014) Hypolipidemic and antiinflammation activities of fermented soybean fibers from Meju in C57BL/6J mice. Phytother Res, 28, 1335-1341 https://doi.org/10.1002/ptr.5134
  13. Lim SI, Son SM (2010) Fermentation properties of low-salted Doenjang supplemented with licorice, mustard, and chitosan. Korean J Food Sci Technol, 42, 323-328
  14. Jung BM (2003) Physicochemical characteristics of freeze dried soybean paste block with sea mustard. Korean J Soc Food Cook Sci, 19, 318-323
  15. Kim EM, Kim YJ, Yu BH, Choi HS, Sin YJ (2012) Quality characteristics of DIY Kochujang with different ratios of rice-nuruk. Korean J Community Living Sci, 147-147
  16. AOAC (1990) Official Methods of Analysis. 15th ed, Association of official analytical chemists, Washington DC, USA, p 335
  17. Cho KM, Kang JR, Kim GM (2014) Quality characteristics of low salted garlic Doenjang during fermentation. Korean J Food Preserv, 21, 627-635 https://doi.org/10.11002/kjfp.2014.21.5.627
  18. Kim JY, Yi YH (2008) pH, acidity, color, reducing sugar, total sugar, alcohol and organoleptic characteristics of puffed rice powder added wheat flour Takju during fermentation. J Food Eng Prog, 12, 71-77
  19. Kim JW, Doo HS, Kwon TH, Kim YS, Shin DH (2011) Quality characteristics of Doenjang and Meju fermented with Aspergillus species and Bacillus subtilis during fermentation. Korean J Food Preserv, 18, 397-406 https://doi.org/10.11002/kjfp.2011.18.3.397
  20. Kim HJ, Lee JJ, Cheigh MJ, Choi SY (1998) Amylase, protease, peroxidase and ascorbic acid oxidase activity of Kimchi ingredients. Korean J Food Sci Technol, 30, 1333-1338
  21. Kim JH, Yoo JS, Lee CH, Kim SY, Lee SK (2006) Quality Properties of soybean pastes made from Meju with mold producing protease isolated from traditional Meju. J Korean Soc Appl Biol Chem, 49, 7-14
  22. National Agricultural Products Quality Management Service (2012) Traditional food quality certification system. p 88
  23. Byun1 MW, Nam1 TG, Chun MS, Lee GH (2014) Physicochemical and sensory characteristics of Doenjang made by traditional methods. J Korean Soc Food Sci Nutr, 43, 1543-1548 https://doi.org/10.3746/jkfn.2014.43.10.1543
  24. Chang M, Kim IC, Chang HC (2010) Effect of solar Salt on the quality characteristics of Doenjang. J Korean Soc Food Sci Nutr, 39, 116-124 https://doi.org/10.3746/jkfn.2010.39.1.116
  25. Lee JY, Mok CK (2010) Changes in physicochemical properties of low salt soybean paste (Doenjang) during fermentation. Korean J Food Eng Prog, 14, 153-158
  26. Mok CK, Song KT, Lee JY, Park YS, Lim SB (2005) Changes in microorganisms and enzyme activity of low salt soybean paste (Doenjang) during fermentation. Korean J Soc Food Eng, 9, 112-117
  27. Lee SY, Park NY, Kim JY, Choi HS (2012) Quality characteristics of rice-Doenjang during fermentation by differently shaped Meju and adding starter. Korean J Food Nutr, 25, 505-512 https://doi.org/10.9799/ksfan.2012.25.3.505
  28. Kim JG (2004) Changes of components affecting organoleptic quality during the ripening of traditional Korean soybean paste-amino nitrogen, amino acids, and color. J Food Hyg Saf, 19, 31-37
  29. Brewing Society of Japan (1999) Component of the alcoholic beverages. Shin Nippon Printing Co Ltd, Tokyo, Japan, p 50-79, 409-418
  30. Mody I, de Koninck Y, Otis TS, Soltesz I (1994) Bridging the cleft at GABA synapses in the brain. Trends Neurosci, 17, 517-525 https://doi.org/10.1016/0166-2236(94)90155-4
  31. Tiedje KE, Stevens K, Barnes S, Weaver DF (2010) ${\beta}$-alanine as a small molecule neurotransmitter. Neurochem Int, 57, 177-188 https://doi.org/10.1016/j.neuint.2010.06.001

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