Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Optimization of microwave-assisted extraction process of Hordeum vulgare L. by response surface methodology

반응표면분석법을 이용한 새싹보리 마이크로웨이브 추출공정의 최적화

  • Lee, Jae-Jun (School of Food Science and Technology, Kyoungpook National University) ;
  • Park, Dae-Hee (School of Food Science and Technology, Kyoungpook National University) ;
  • Lee, Won-Young (School of Food Science and Technology, Kyoungpook National University)
  • Received : 2017.10.16
  • Accepted : 2017.11.21
  • Published : 2017.11.30
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Abstract

This study attempted to find optimum extract range of active ingredient for barley sprouts (Hordeum vulgare L.). Extracts from Hordeum vulgare L. were made by microwave extraction method and total polyphenol content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DPPH) were measured with extract of Hordeum vulgare L.. Response surface methodology (RSM) was applied to a extraction process, and central composite design (CCD) was also used for this process to examine the optimum condition. Independent variables ($X_n$) are concentration of ethanol ($X_1$: 0, 25, 50, 75, 100%), microwave power ($X_2$: 60, 120, 180, 240, 300 W), extraction time ($X_3$: 4, 8, 12, 16, 20 min). Dependent variables ($Y_n$) are TPC ($Y_1$), TFC ($Y_2$), DPPH radical scavenging ($Y_3$). It is formed by sixteen conditions to extract. The $R^2$ value of dependent variables is ranged from 0.90 to 0.97 (p<0.05). Experiments values within the optimal range (40% of ethanol concentration, 120 W of microwave power, 18 min of extraction time) were 3.74 mg GAE/g (TPC), 3.00 mg RE/g (TFC), 35.43% (DPPH), respectively. Under the optimized conditions, predicted value showed no significant difference comparing with the experimental values.

본 연구는 새싹보리를 이용하여 제품 개발에 적용할 수 있도록, 생리활성물질의 최적 추출구간을 설정하는 데에 목적이 있다. 에탄올 농도(0-100%), 마이크로웨이브 전력(60-300 W), 추출시간(4-20분)을 종속 변수로 설정한 후, 빠르고 추출 수율이 좋은 마이크로웨이브추출법을 이용하여 16구의 다른 추출 조건을 중심합성계획법에 따라 설정하여 새싹보리를 추출하였다. 이 후, 추출물의 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH 라디칼 소거능 활성을 측정하였다. 모든 회귀식의 $R^2$는 0.9 이상으로 5% 수준 이내에서 유의성이 인정되었다. 총 폴리페놀의 최적 추출조건은 에탄올 농도 58.94%, 마이크로웨이브 전력 209.04 W, 추출시간은 18.17분으로 나타났으며, 총 플라보노이드의 최적 추출 조건은 에탄올 농도 52.7%, 마이크로웨이브 전력 73.03 W, 추출시간은 5분으로 나타났다. DPPH 라디칼소거능 활성의 경우, 에탄올 농도 75.84%, 마이크로웨이브 전력 210.79 W, 추출시간 은 6.5분으로 나타났다. 조건에 따른 TPC, TFC 그리고 DPPH 라디칼 소거능 활성의 예측값은 각각 3.84 mg GAE/g, 3.00 mg RE/g 그리고 35.43%의 수치를 나타냈다. 최적 범위 내 임의의 점, 즉 에탄올 농도 40%, 마이크로웨이브 전력 120 W, 추출시간은 18분에서 실험값은 3.38 mg GAE/g, 2.64 mg RE/g, 그리고 37.94%를 나타냈으며 예측값과 실제 실험값은 유사한 값을 보였다.

Keywords

References

  1. Jun SY, Kim TH, Hwang SH (2012) The consumption status and preference for sprouts and leafy vegetables. Korean J Food Preserv, 19, 783-791 https://doi.org/10.11002/kjfp.2012.19.5.783
  2. Yoo KM (2015) A opinion on the growth & revitalization of health functional food market. J Korean Soc Food Sci Nutr, 20, 4-7
  3. Lee MY, Shin SL, Chang YD, Lee CH (2009) Environment factors for germination, growing and storage of sprout vegetables of Coreopsis tinctoria Nutt., Saussurea pulchella (Fisch.) Fisch. and Matricaria recutica L.. Korean J Plant Res, 22, 136-144
  4. Kim JS, Cho JS, Lee CH (2015) Effect of environmental factors on sprout germination, growth, and storage of six Aster species. Korean J Hortic Sci Technol, 33, 638-646 https://doi.org/10.7235/hort.2015.14147
  5. Seo MW (2013) 21. vegetables Sprouts. Hortic Environ Biotechnol, 123-126
  6. Kang HM, Kim JG (2013) 4. vegetables Sprouts, Fresh-cutting and processing. Hortic Environ Biotechnol, 555-558
  7. Lee YH, Kim JH, Kim SH, Oh JY, Seo WD, Kim KM, Jung JC, Jung YS (2016) Barley sprouts extract attenuates alcoholic fatty liver injury in mice by reducing inflammatory response. Nutrients, 8, 440 https://doi.org/10.3390/nu8070440
  8. Kim AR, Lee MY, Lee JJ (2012) Effect of barley leaf ethanol extract on lipid metabolism in rats fed high cholesterol diet. Bull Anim Biotechnol, 4, 15-22
  9. Byun AR, Chun HJ, Lee J, Lee SW, Lee HS, Shim KW (2015) Effects of a dietary supplement with barley sprout extract on blood cholesterol metabolism. J Evidance Based Complementary Altern Med, 7
  10. Kim DC, Kim DW, Lee SD, In MJ (2006) Preparation of barley leaf powder tea and its quality characteristics. J Korean Soc Food Sci Nutr, 35, 734-737 https://doi.org/10.3746/jkfn.2006.35.6.734
  11. Moon JH (2013) Growth characteristics of barley (Hordeum Vulgare L.) and Wheat (Triticum aestivum L.) sprouts according to light quality of LED. MS Thesis, Kong Ju National University, Korea, p 3-6
  12. Eun CS, Hwang EY, Lee SO, Yang SA, Yu MH (2016) Anti-oxidant and anti-inflammatory activities of barley sprout extract. J Life Sci, 26, 537-544 https://doi.org/10.5352/JLS.2016.26.5.537
  13. Lee JH (2015) Effects of barley sprout and its compounds, hexacosanol and saponarin, on cholesterol and glucose metabolism. Ph D Thesis, Korea University, Korea, p 159-161
  14. Lee J (2013) Effects of a dietary supplement extracted from barley sprouts on cholesterol metabolism. Ph D Thesis, Ewha Womans University, Korea, p 1-4
  15. Bang HR (2017) The effects of barley sprouts(Hordeum Vulgare L.) extract on bone metabolism in ovariectomized rats. Kyung Hee University, MS Thesis, Korea, p 56-65
  16. Park MJ (2013) Neuraminidase inhibitory activity of polyphenolic compounds from the barley sprouts (Hordeum Vulgare L.). Pusan National University, MS Thesis, Korea, p 121-122
  17. Park SE, Seo SH, Kim EJ, Lee KM, Son HS (2017) Quality characteristics of string cheese prepared with barley sprouts. J Korean Soc Food Sci Nutr, 46, 841-847
  18. Lee KS (2015) Analysis of consumer's present use of sprout vegetables and quality characteristics of grain sourdough and barley sprouts. Catholic University, Ph D Thesis, Korea, p 125-128
  19. Joo EY, Kim NW (2008) Polyphenol contents and antioxidant activity of extracts from Angelica dahurica root after different conditions of microwave-assisted extraction. Korean J Food Preserv, 15, 133-138
  20. Kwak DH (2001) Determination of food safety in the process for the preparation of edible oil by the analysis of residual organic solvent. Seoul National University of Technology, MS Thesis, Korean, p 1-2
  21. Kwon JH, Kim KE, Lee GD (2000) Optimization of microwave-assisted extraction under atmospheric pressure condition for soluble ginseng components. Korean J Food sci Technol, 32, 117-124
  22. Lee HJ, Do JR, Kwon JH, Kim HK (2011) Physiological properties of oak mistletoe (Loranthus yadoriki) extracts by microwave extraction condition. Korean J Food Preserv, 18, 72-78 https://doi.org/10.11002/kjfp.2011.18.1.072
  23. Lim SB, Kim SH, Ko YH, Oh CK, Oh MC, Ko YG, Park CS (1995) Extraction yields of Hizikia fusiforme and Aloe vera Linne by supercritical carbon dioxide and antimicrobial activity of their extracts. Korean J Food Sci Technol, 27, 68-73
  24. Lee EJ, Yang SA, Choi HD, Im HG, Whang K, Lee IS (2011) Comparison of gingerols in various fractions and the antioxidant effects of supercritical fluid extracts from ginger. Korean J Food Sci Technol, 43, 469-474 https://doi.org/10.9721/KJFST.2011.43.4.469
  25. Park EJ, Ahn JJ, Kim JS, Kwon JH (2013) Antioxidant activities in freeze-dried and hot air-dried schizandra fruit (Schizandra chinensis Baillon) at different microwaveassisted extraction conditions. Korean J Food Sci Technol, 45, 667-674 https://doi.org/10.9721/KJFST.2013.45.6.667
  26. Lee SY, Shin SR, Kim KS, Kwon JH (2000) Establishment of extraction conditions for effective components from Angelica gigas Nakai using microwave-assisted process. J Korean Soc Food Sci Nutr, 29, 442-447
  27. Lee SB, Lee GD, Kwon JH (1999) Optimization of extraction conditions for soluble ginseng components using microwave extraction system under pressure. J Korean Soc Food Sci Nutr, 28, 409-416
  28. Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA (2008) Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta, 76, 965-977 https://doi.org/10.1016/j.talanta.2008.05.019
  29. Jeong JE, Shim SP, Jeong YS, Jung HK, Kim YC, Hong JH (2011) Optimization of extraction conditions for ethanol extracts from Citrus unshiu peel by response surface methodology. Korean J Food Preserv, 18, 755-763 https://doi.org/10.11002/kjfp.2011.18.5.755
  30. Park DH, Lee JJ, Park JJ, Park SH, Lee WY (2017) Optimization of microwave-assisted extraction process for blue honeysuckle (Lonicera coerulea L.) using response surface methodology. Korean J Food Preserv, 24, 623-630 https://doi.org/10.11002/kjfp.2017.24.5.623
  31. Lee HJ, Do JR, Kwon JH, Kim HK (2012) Optimization of Corni fructus extracts by response surface methodology. J Korean Soc Food Sci Nutr, 41, 390-395 https://doi.org/10.3746/jkfn.2012.41.3.390
  32. Pare JRJ, Sigouin M, Lapoinate J (1991) Microwaveassisted natural products extraction. US Patent No. 5002784 A
  33. Min DL, Lim SW, Ahn JB, Choi YJ (2010) Optimization of ethanol extraction conditions for antioxidants from Zizyphus jujuba Mill. leaves using response surface methodology. Korean J Food Sci Technol, 42, 733-738
  34. Lee EJ, Kwon YJ, Noh JE, Lee JE, Lee SH, Kim JK, Kim KS, Choi YH, Kwon JH (2005) Optimization of microwave-assisted process for extraction of effective components from Mosla dianthera M.. Korean J Food Preserv, 12, 617-623
  35. Lee SJ, Kang JR, Kim JG, Kang SK, Sung NJ (2013) Establishment of optimum extraction condition for antioxidant activity of Artemisia annua L. by response surface methodology. J Agric Life Sci, 47, 103-113
  36. Kim DI, Hong JH (2012) Optimization of ethanol extraction conditions for functional components from Lespedeza cuneata using response surface methodology. Korean J Food Cook Sci, 28, 275-283 https://doi.org/10.9724/kfcs.2012.28.3.275
  37. Han SY (2011) Antioxidative effects of the extract from barley sprout and application of the extracts to an edible oil and fat. MS Thesis, Chonbuk National University, Korea, p 34-36

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