DOI QR코드

DOI QR Code

Effect of particle size of naked oat flours on physicochemical and antioxidant property

쌀귀리 가루의 입도별 이화학적 특성 및 항산화 활성

  • Jun, Hyun-Il (Department of Food Science and Technology, Chonbuk National University) ;
  • Yoo, Sun-Hee (Agricultural Technology Center) ;
  • Song, Geun-Seoup (Department of Food Science and Technology, Chonbuk National University) ;
  • Kim, Young-Soo (Department of Food Science and Technology, Chonbuk National University)
  • Received : 2017.11.09
  • Accepted : 2017.11.23
  • Published : 2017.11.30

Abstract

This study was carried out to investigate the effect of particle size of naked oat flour (NOF) on physicochemical property and antioxidant activity. The NOF was passed through $250{\mu}m$ and $160{\mu}m$ size sieves to obtain three fractions (fraction A: $250{\mu}m$ or more, fraction B: $160-250{\mu}m$, and fraction C: $160{\mu}m$ or less). Moisture, crude protein, crude fat, and crude ash contents of NOF were 8.4, 15.7, 10.0, and 1.8%, respectively, and these contents were increased as the particle size of NOF decreased. The mineral and free amino acid contents of NOF also had a similar tendency. The contents of total starch, amylose, starch damage, total dietary fiber, ${\beta}$-glucan, total phenolics, and flavonoids in NOF were 56.4%, 21.4%, 11.7%, 11.0%, 4.7%, $237.8{\mu}g/g$ and $90.9{\mu}g/g$, respectively. As the particle size of NOF decreased, total starch, amylose, and starch damage contents increased, whereas total dietary fiber, ${\beta}$-glucan, total phenolic and flavonoid contents decreased. Also, three antioxidant activities of NOF were closely correlated with their total phenolics and flavonoids contents, showing high correlation coefficient values ($R^2=0.87$ and 0.81, respectively).

본 연구에서는 영양학적 가치가 높은 쌀귀리의 식품산업 이용률을 높이고자 쌀귀리를 입도별로 제조하여 이들의 이화학적 특성 및 항산화 활성을 비교하였다. 쌀귀리의 조단백질, 조지방 및 조회분 함량이 각각 15.7%, 10.0% 및 1.8%이었으며, 입도가 작아질수록 이들의 함량은 감소하였다. 주요 유리아미노산은 비필수아미노산 중에서는 cystine(58.3 mg%), ${\beta}$-aminoisobutyric acid(53.8 mg%) 등, 필수아미노산 중에서는 phenylalanine(8.6 mg%)과 histidine (12.2 mg%)이었으며, 입도가 작아질수록 함량은 감소하였다. 총 전분, 아밀로스, 손상전분, 총 식이섬유, ${\beta}$-glucan 및 총 유리당 함량은 각각 56.4%, 21.4%, 11.7%, 11.0%, 4.7% 및 2,555.3 mg%이었으며, 입도가 작아질수록 총 전분, 아밀로스 및 손상전분의 함량은 감소한 반면에 총 식이섬유와 ${\beta}$-glucan의 함량은 증가하였다. 색도는 L 값이 75.3, a 값이 0.3 및 b 값이 13.1이었으며, 입도가 작아질수록 L 값은 증가하였으나 a와 b 값은 감소하였다. 호화특성은 최고점도가 263.8 RVU, 최저점도가 155.6 RVU 및 최종점도가 399.3 RVU이었으며, 입도가 작아질수록 최고점도와 최종점도는 증가하였다. TPC, TFC 및 항산화 활성(DPPH radical assay, ABTS radical cation assay 및 reducing power)은 각각 $237.8{\mu}g/g$, $90.9{\mu}g/g$, 57.3%, 53.4% 및 0.23이었으며, 입도가 작아질수록 항산화 성분 및 항산화 활성은 감소하였다. 한편 TPC와 TFC에 관한 각 항산화 활성의 결정계수(correlation determination coefficient, $R^2$) 값이 각각 0.87 이상과 0.81 이상으로 나타나 항산화 성분과 항산화 활성은 상관성을 보였다.

Keywords

References

  1. Zhou M, Robards K, Glennie-Holmes M, Helliwell S (1999) Oat lipids. J Am oil Chem Soc, 76, 159-169 https://doi.org/10.1007/s11746-999-0213-1
  2. Manolache FA, Hanganu A, Duta DE, Belc N, Marin DI (2013) The physico-chemical and spectroscopic composition characterization of oat grains and oat oil samples. Rev Chim, 64, 45-48
  3. Biel W, Bobko K, Maciorowski R (2009) Chemical composition and nutritive value of husked and naked oats grain. J Cereal Sci, 49, 413-418 https://doi.org/10.1016/j.jcs.2009.01.009
  4. Pomeranz Y, Youngs VL, Robbins GS (1973) Protein content and amino acid composition of oat species and tissues. Cereal Chem, 50, 702-707
  5. Lee YT (1996) Physicochemical characteristics and physiological functions of ${\beta}$-glucans in barley and oats. Korean J Crop Sci, 41, 10-24
  6. Han OK, Park HH, Park TI, Seo JH, Park KH, Kim JG, Heo HY, Hong YG, Kim DH (2008) A new early-heading and high-yielding naked oat cultivar for human consumption, 'Choyang'. Korean J Breed Sci, 40, 512-516
  7. KMFDS (2016) Analysis report for annual production of functional food in 2016. Korean Ministry of Food and Drug Safety, Seoul, Korea, p 1-12
  8. Lee NY (2013) Starch and quality characteristic of Korean rice cultivar with waxy and non-waxy type. Korean J Crop Sci, 58, 226-231 https://doi.org/10.7740/kjcs.2013.58.3.226
  9. Son CG (2014) Progress of functional food in Korea and strategy of Korean medicine. J Korean Med, 35, 68-74 https://doi.org/10.13048/jkm.14007
  10. Seo YK, Whang K (1998) In vitro cholesterol adsorption activity of oat gum. J Korean Soc Food Sci Nutr, 27, 785-788
  11. Dhingra D, Michael M, Rajput H, Patil RT (2012) Dietary fibre in foods: a review. J Food Sci Technol, 49, 255-266 https://doi.org/10.1007/s13197-011-0365-5
  12. Gray DA, Auerbach RH, Hill S, Wang R, Campbell, GM, Webb C, South JB (2000) Enrichment of oat antioxidant activity by dry milling and sieving. J Cereal Sci, 32, 89-98 https://doi.org/10.1006/jcrs.2000.0318
  13. Jeong HS, Kang TS, Jung IS, Park HJ, Min YK (2003) ${\beta}$-glucan contents with different particle size and varieties of barley and oats. Korean J Food Sci Technol, 35, 610-616
  14. Peterson DM, Emmons CL, Hibbs AH (2001) Phenolic antioxidant and antioxidant activity in pearling fractions of oat groats. J Cereal Sci, 33, 97-103 https://doi.org/10.1006/jcrs.2000.0347
  15. AOAC (1996) Official Methods of Analysis. 15th ed, Association of Official Analytical Chemists, Washington DC, USA, p 1121-1180
  16. KFDA (2005) Korea Food and Drug Administration. Food Code. Korean Foods Industry Association, Seoul, Korea, p 358-364
  17. Spackman DH, Stein WH, Moore S (1958) Automatic recording apparatus for use in chromatography of amino acids. Anal chem, 30, 1190-1206 https://doi.org/10.1021/ac60139a006
  18. McCleary BV, Solah V, Gibson TS (1994) Quantitative measurement of total starch in cereal flours and products. J Cereal Sci, 20, 51-58 https://doi.org/10.1006/jcrs.1994.1044
  19. Williams WB, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensmn Wiss Technol, 28, 25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  20. Boyaci IH, Williams PC, Koksel H (2004) A rapid method for the estimation of damaged starch in wheat flour. J Cereal Sci, 39, 139-145 https://doi.org/10.1016/j.jcs.2003.09.002
  21. Prosky L, Asp NG, Schweizer TF, Devries JW, Furdal I (1998) Determination of insoluble, soluble and total dietary fiber in foods and food products, Interlaboratory study. J Assoc Off Anal Chem, 71, 1017-1025
  22. McCleary BV, Glennie-Holmes M (1985) Enzymic quantification of (1$\rightarrow$3),(1$\rightarrow$4)-${\beta}$-D-glucan in barley and malt. J Inst Brew, 91, 285-295 https://doi.org/10.1002/j.2050-0416.1985.tb04345.x
  23. Cho IK, Jin SW, Kim YD (2009) Analysis of components in the parts of Opuntia ficus indica from Shinan Korea. Korean J Food Preserv, 16, 742-746
  24. Cha MN, Jun Hl, Song GS, Kim YS (2012) The Effects of germination conditions on GABA and the nutritional components of barley. J Food Sci Technol, 44, 41-47
  25. Dewanto V, Wu X, Adonm KK, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem, 50, 3010-3014 https://doi.org/10.1021/jf0115589
  26. Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and they scavenging effects on superoxide radicals. Food Chem, 64, 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  27. Williams WB, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol, 28, 25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  28. Arts MJTJ, Haenen GRMM, Voss HP, Bast A (2004) Antioxidant capacity of reaction products limits the applicability of the trolox equivalent antioxidant capacity (TEAC) assay. Food Chem Toxicol, 42, 45-49 https://doi.org/10.1016/j.fct.2003.08.004
  29. Oyaizu M (1986) Studies on products of browning reaction-antioxidant activities of products of browning reaction prepared from glucoamin. Japanese J Nutr Diet, 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  30. SAS (2004) SAS/STAT 9.1 User's Guide. SAS Institute Inc, Cary, NC, USA, p 421-480
  31. Kim HY, Hwang IG, Woo KS, Kim KH, Kim KJ, Lee CK, Lee JS, Jeong HS (2010) Chemical components changes of winter cereal crops with germination. J Korean Soc Food Sci Nutr, 39, 1700-1704 https://doi.org/10.3746/jkfn.2010.39.11.1700
  32. Han OK, Park HH, Heo HY, Park TI, Seo JH, Park KH, Kim JG, Hong YG, Kim DH (2008) A new naked oat cultivar for human consumption, 'Daeyang' with high-yielding and good-quality. Korean J Breed Sci, 41, 56-60
  33. Hahn JD, Chung TK, Baker DH (1990) Nutritive value of oat flour and oat bran. J Anim Sci, 68, 4253-4260 https://doi.org/10.2527/1990.68124253x
  34. Kosieradzka I, Fabijanska M (2001) Comparison of the nutritive value of naked and husked oat protein with wheat and maize. J Anim Feed Sci, 10, 309-314
  35. Hoover R, Senanayake SPJN (1996) Composition and physicochemical properties of oat starches. Food Res Int, 29, 15-26 https://doi.org/10.1016/0963-9969(95)00060-7
  36. Tian B, Xie B, Shi J, Wu J, Cai Y, Xu T, Xue SJ, Deng Q (2010) Physicochemical changes of oat seeds during germination. Food Chem, 119, 1195-1200 https://doi.org/10.1016/j.foodchem.2009.08.035
  37. Seo YK, Whang K (1998) In vitro cholesterol adsorption activity of oat gum. J Korean Soc Food Sci Nutr, 27, 785-788
  38. Jun HI, Yang EJ, Kim YS, Song GS (2008) Effect of dry and wet millings on physicochemical properties of black rice flours. J Korean Soc Food Sci Nutr, 37, 900-907 https://doi.org/10.3746/jkfn.2008.37.7.900
  39. Meuser F, Klingler RW, Niediek EK (1978) Characterization of mechanically modified starch. Starch, 30, 376-384 https://doi.org/10.1002/star.19780301105
  40. Leonova S, Shelenga T, Hamberg M, Konarev AV, Loskutov I, Carlsson AS (2008) Analysis of oil composition in cultivars and wild species of oat (Avena sp.). J Agric Food Chem, 56, 7983-7991 https://doi.org/10.1021/jf800761c
  41. Kim CH, Tie J, Ryu GH (2012) Effect of moisture content on physical properties of extruded creal flours. J Korean Soc Food Sci Nutr, 41, 1603-1610 https://doi.org/10.3746/jkfn.2012.41.11.1603
  42. Lee YT, Seog HM, Cho MK, Kim SS (1996) Physicochemical properties of hull-less barley flours prepared with different ginding mills. Korean J Food Sci Technol, 28, 1078-1083
  43. Ovando-Martinez M, Whitney K, Reuhs BL, Doehlert DC, Simsek S (2013) Effect of hydrothermal treatment on physicochemical and digestibility properties of oat starch. Food Res Int, 52, 17-25 https://doi.org/10.1016/j.foodres.2013.02.035
  44. Brindzova L, Certik M, Rapta P, Zalibera M, Mikulajova A, Takacsova M (2008) Antioxidant activity, ${\beta}$-glucan and lipid contents of oat varieties. Czech J Food Sci, 26, 163-173 https://doi.org/10.17221/2564-CJFS
  45. Yu L, Perret J, Davy B, Wilson J, Melby CL (2002) Antioxidant properties of cereal products. J Food Sci, 67, 2600-2603 https://doi.org/10.1111/j.1365-2621.2002.tb08784.x
  46. Peterson DM (2001) Oat antioxidants. J Cereal Sci, 33, 115-129 https://doi.org/10.1006/jcrs.2000.0349
  47. Guo X, Yao H (2006) Fractionation and characterization of tartary buckwheat flour proteins. Food Chem, 98, 90-94 https://doi.org/10.1016/j.foodchem.2005.05.055

Cited by

  1. Bread-making properties of rice bread added with naked oat flours vol.26, pp.1, 2017, https://doi.org/10.11002/kjfp.2019.26.1.68
  2. Nutritional composition and physicochemical properties of oat flour sieving fractions with different particle size vol.154, pp.None, 2017, https://doi.org/10.1016/j.lwt.2021.112757