Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-wrinkle effects of extracts and solvent fractions from Nymphoides peltata on CCD-986sk

CCD-986sk 세포내에서의 노랑어리연꽃 추출물 및 분획물의 주름개선 효과

  • Kim, You Ah (Skin Science Research Institute, Kolmar Korea Co., Ltd.) ;
  • Kim, Dong Hee (Applied Product Development Team, National Development Institute of Korean Medicine) ;
  • Yu, Jae Myo (Applied Product Development Team, National Development Institute of Korean Medicine) ;
  • Park, Chae Bin (Skin Science Research Institute, Kolmar Korea Co., Ltd.) ;
  • Park, Tae soon (Applied Product Development Team, National Development Institute of Korean Medicine) ;
  • Park, Byoung Jun (Skin Science Research Institute, Kolmar Korea Co., Ltd.)
  • Received : 2017.08.17
  • Accepted : 2017.10.11
  • Published : 2017.12.01
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Abstract

The purpose of this study was to investigate the antiaging effect in the fibroblasts by using the extracts and fractions of Nymphoides peltata for the development of new functional cosmetic materials. The anti-aging effects of the extracts and fractions of N. peltata were measured using CCD-986sk cells. Ethyl aceate (EA) fraction inhibited UVB-induced reactive oxygen species expression by more than 50%, and n-butyl alcohol (BuOH) fraction increased expression of pro-collagen mRNA by more than 50% at $25{\mu}g/mL$. In addition, EA and BuOH fractions inhibited MMP-1 protein and mRNA expression in a concentration-dependent manner, BuOH fraction inhibited MMP-1 mRNA expression by more than 40% at $10{\mu}g/mL$. Especially, the high efficacy of EA and BuOH of the N. peltata was confirmed. Therefore, the EA and BuOH of N. peltata are expected to be useful as anti-aging materials in cosmetics field.

본 연구는 새로운 기능성 화장품 소재 개발을 위해 노랑어리연꽃 추출물 및 분획물을 이용하여 섬유아세포내에서의 항노화 효과를 확인 하고자 하였다. 노랑어리연꽃 추출물 및 분획물의 CCD-986sk 세포를 이용하여 항노화 효과를 측정한 결과, EA 분획물이 UVB에 의해 증가된 ROS 발현을 50% 이상 억제하였으며, BuOH 분획물의 경우 pro-collagen mRNA 발현을 $25{\mu}g/mL$에서 50% 이상 발현을 증가시켰다. EA 분획물과 BuOH 분획물 모두 MMP-1 protein과 mRNA발현을 농도 의존적으로 저해시켰으며, BuOH 분획물의 경우 $10{\mu}g/mL$에서 40% 이상의 MMP-1 mRNA 발현 억제율을 확인하였다. 이는 노화 인자인 활성산소종의 생성 억제와 주름과 밀접한 관련이 있는 콜라겐의 합성 및 분해억제를 통해 항산화 및 주름 개선 효과가 있는 것으로 판단되었으며 특히 EA 와 BuOH 분획물의 높은 효능이 확인되었다. 따라서 노랑어리연꽃 EA 및 BuOH 분획물은 화장품 분야에서 항노화 소재로서의 활용가치가 있을 것으로 사료된다.

Keywords

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