DOI QR코드

DOI QR Code

Effects of titanium oxide nanoparticles on Oryzias latipes embryos and sac-fry under different irradiation conditions

  • Nam, Sun-Hwa (Department of Environmental Health Science, Konkuk University) ;
  • Shin, Yu-Jin (Department of Environmental Health Science, Konkuk University) ;
  • An, Youn-Joo (Department of Environmental Health Science, Konkuk University)
  • 투고 : 2017.05.04
  • 심사 : 2017.06.18
  • 발행 : 2017.12.31

초록

Some phototoxicity of titanium dioxide nanoparticles ($TiO_2$ NPs) has been reported in recent years in studies with fish embryos or larvae. However, it is necessary to focus on the potential effects of embryonic exposure due to irreversible abnormalities and mortalities observed in sac-fry, and to expand various fish embryos to generate multiple test species. The aim of this study was to evaluate the effects of $TiO_2$ NPs under different irradiation conditions in exposed Oryzias latipes (O. latipes) at the embryonic and sac-fry stages. The effects of different irradiation conditions were observed using ultra-violet (UV) and visible light, and the corresponding effects were monitored by determining cumulative mortality and abnormality. O. latipes were exposed for 8 d to 0, 1, 5, 10, or 50 mg/L $TiO_2$ NPs under UV ($4,818.86mW/m^2$ at the bottom of clear vials) or visible light, after which the embryos were transferred to NP-free embryo-rearing solution until 16 days post fertilization (dpf). Abnormalities of embryos and sac-fry increased at high $TiO_2$ NP concentrations under UV irradiation, compared to control samples treated with visible light or UV irradiation alone. This work provides information regarding the phototoxicity of $TiO_2$ NPs using O. latipes at the embryonic and sac-fry stages.

키워드

참고문헌

  1. Clemente Z, Castro VLSS, Moura MAM, Jonsson CM, Fraceto LF. Toxicity assessment of $TiO_2$ nanoparticles in zebrafish embryos under different exposure conditions. Aquat. Toxicol. 2014;147:129-139. https://doi.org/10.1016/j.aquatox.2013.12.024
  2. Boxall A, Chaudhry Q, Sinclair C, et al. Current and future predicted environmental exposure to engineered nanoparticles. Central Science Laboratory; 2007.
  3. Wang H, Wick RL, Xing B. Toxicity of nanoparticulate and bulk ZnO, $Al_2O_3$ and $TiO_2$ to the nematode Caenorhabditis elegans. Environ. Pollut. 2009;157:1171-1177. https://doi.org/10.1016/j.envpol.2008.11.004
  4. Bar-Ilan O, Louis KM, Yang SP, et al. Titanium dioxide nanoparticles produce phototoxicity in the developing zebrafish. Nanotoxicology 2012;6:670-679. https://doi.org/10.3109/17435390.2011.604438
  5. Faria M, Navas JM, Soares AMVM, Barata C. Oxidative stress effects of titanium dioxide nanoparticle aggregates in zebrafish embryos. Sci. Total Environ. 2014;470-471:379-389. https://doi.org/10.1016/j.scitotenv.2013.09.055
  6. Clemente Z, Castro VL, Feitosa LO, et al. Fish exposure to nano-$TiO_2$ under different experimental conditions methodological aspects for nanoecotoxicology investigations. Sci. Total Environ. 2013;463-464:647-656. https://doi.org/10.1016/j.scitotenv.2013.06.022
  7. Li S, Pan X, Wallis LK, Fan Z, Chen Z, Diamond SA. Comparison of $TiO_2$ nanoparticle and graphene-$TiO_2$ nanoparticle composite phototoxicity to Daphnia magna and Oryzias latipes. Chemosphere 2014;112:62-69. https://doi.org/10.1016/j.chemosphere.2014.03.058
  8. Ma H, Brennan A, Diamond SA. Photocatalytic reactive oxygen species production and phototoxicity of titanium dioxide nanoparticles are dependent on the solar ultraviolet radiation spectrum. Environ. Toxicol. Chem. 2012a;31:2099-2107. https://doi.org/10.1002/etc.1916
  9. Ma H, Brennan A, Diamond SA. Phototoxicity of $TiO_2$ nanoparticles under solar radiation to two aquatic species: Daphnia magna and Japanese medaka. Environ. Toxicol. Chem. 2012b;31:1621-1629. https://doi.org/10.1002/etc.1858
  10. Shin YJ, Nam SH, An YJ. Continuous UV irradiation increases the adverse effects of photoreactive nanoparticles on the early development of Oryzias latipes. Environ. Toxicol. Chem. 2015;35:1195-1200.
  11. Lindsey CF, Erik JF, Yuhe H, Greg GG. Poly(acrylic acid)-coated titanium dioxide nanoparticle and ultraviolet light co-exposure has minimal effect on developing zebrafish (Danio rerio). Environ. Sci. Nano. 2017;4:658-669. https://doi.org/10.1039/C6EN00436A
  12. Lee WM, An YJ. Effects of zinc oxide and titanium dioxide nanoparticles on green algae under visible, UVA, and UVB irradiations: No evidence of enhanced algal toxicity under UV pre-irradiation. Chemosphere 2013;91:536-544. https://doi.org/10.1016/j.chemosphere.2012.12.033
  13. Kim SW, An YJ. Effect of ZnO and $TiO_2$ nanoparticles preilluminated with UVA and UVB light on Escherichia coli and Bacillus subtilis. Appl. Microbiol. Biotechnol. 2012;95:243-253. https://doi.org/10.1007/s00253-012-4153-6
  14. Ma H, Diamond SA, Phototoxicity of $TiO_2$ nanoparticles to zebrafish (Danio rerio) is dependent on life stage. Environ. Toxicol. Chem. 2013;32:2139-2143. https://doi.org/10.1002/etc.2298
  15. Shin YJ, Nam SH, An YJ. Japanese medaka exposed to gold nanoparticles: Only embryonic exposure generates irreversible hatching failure, developmental failure, and mortality of sac-fry. Comp. Biochem. Phys. C. 2014;161:26-32.
  16. Truong L, Saili KS, Miller JM, Hutchison JE, Tanguay RL. Persistent adult zebrafish behavioral deficits results from acute embryonic exposure to gold nanoparticles. Comp. Biochem. Phys. C. 2012;155:269-274.
  17. Organization for Economic Co-operation and Development (OECD). Guideline for testing of chemicals no. 212. Fish, short-term toxicity test on embryo and sac-fry stages. 1998.
  18. Kirchen RV, West WR. The Japanese medaka: Its care and development [Ichthyology; Fishes]. Burlington, North Carolina: Carolina Biological Supply Co.; 1976.
  19. Shen W, Li Z, Wang H, Liu Y, Guo Q, Zhang Y. Photocatalytic degradation for methylene blue using zinc oxide prepared by codeposition and sol-gel methods. J. Hazard. Mater. 2008;152:172-175. https://doi.org/10.1016/j.jhazmat.2007.06.082

피인용 문헌

  1. Surface modified nanostructured-TiO2 thin films for removal of Congo red vol.35, pp.10, 2018, https://doi.org/10.1007/s11814-018-0114-9