대한의생명과학회지 (Biomedical Science Letters)

  • 제23권4호
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  • Pages.411-415
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  • 2017
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
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Anti-neuroinflammatory Effect of Plantago Major var. Japonica in BV-2 Microglial Cells

  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
  • 투고 : 2017.09.26
  • 심사 : 2017.11.21
  • 발행 : 2017.12.31
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초록

To evaluate the protective effects of Plantago Major extract (PME) in stimulated BV-2 microglial cells and its anti-oxidant properties, cell viability assessment was performed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to activate BV-2 microglia. Nitric oxide (NO) levels were measured using Griess assay. Tumor necrosis factor-alpha (TNF-${\alpha}$) production was evaluated by enzyme-linked immunosorbent assay (ELISA). Antioxidant properties were evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. LPS-activated excessive release of NO in BV-2 cells was significantly inhibited by PME (P < 0.001 at $100{\mu}g/mL$). PME also scavenged DPPH radicals in a dose-dependent manner (P < 0.05 at $10{\mu}g/mL$ and P < 0.001 at $20{\sim}200{\mu}g/mL$). These results indicate that PME attenuated neuroinflammatory responses in LPS-activated BV-2 microglia by inhibiting excessive production of pro-inflammatory mediators such as NO and TNF-${\alpha}$. The anti-neuroinflammatory potential of PME may be related to its strong antioxidant properties.

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참고문헌

  1. Bruno R, Dugan C, Smyth J, DiNatale D, Koo S. Green tea extract protects leptin-deficient, spontaneously obese mice from hepatic steatosis and injury. American Society for Nutrition. 2008. 21: 323-331.
  2. Beara IN, Lesjak MM, Jovin ED, Balog KJ, Anackov GT, Orcic DZ. Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. Journal of Agricultural and Food Chemistry. 2009. 57: 9268-9273. https://doi.org/10.1021/jf902205m
  3. Chiang LC, Chiang W, Chang MY, Lin CC. In vitro cytotoxic, antiviral and immunomodulatory effects of Plantago Major and Plantago asiatica. The American Journal of Chinese Medicine. 2003. 31: 225-234. https://doi.org/10.1142/S0192415X03000874
  4. Finkel T, Holbrook NJ. Oxidants. oxidative stress and the biology of ageing. Nature. 2000. 408: 239-247. https://doi.org/10.1038/35041687
  5. Frankola KA, Greig NH, Luo W, Tweedie D. Targeting TNF-alpha to elucidate and ameliorate neuroinflammation in neurodegenerative diseases. CNS & Neurological Disorders Drug Targets. 2011. 10: 391-403. https://doi.org/10.2174/187152711794653751
  6. Gao HM, Jiang J, Wilson B, Zhang W, Hong JS, Liu B. Microglial activation-mediated delayed and progressive degeneration of rat nigral dopaminergic neurons: relevance to Parkinson's disease. Journal of Neurochemistry. 2002. 81: 1285-1297. https://doi.org/10.1046/j.1471-4159.2002.00928.x
  7. Gomez-Flores R, Calderon CL, Scheibel LW, Tamez-Guerra P, Rodriguez-Padilla C, Tamez-Guerra R. Immuno-enhancing properties of Plantago Major leaf extract. Phytotherapy Research. 2000. 14: 617-622. https://doi.org/10.1002/1099-1573(200012)14:8<617::AID-PTR674>3.0.CO;2-N
  8. Kim BW, Koppula S, Kim IS, Lim HW. Antineuroinflammatory activity of Kamebakaurin from Isodon japonicus via inhibition of c-Jun NH-terminal kinase and p38 mitogen-activated protein kinase pathway in activated microglial cells. Journal of Pharmacological Sciences. 2011. 116: 296-308. https://doi.org/10.1254/jphs.10324FP
  9. Kim SH, Smith CJ, Van Eldik LJ. Importance of MAPK pathways for microglial pro-inflammatory cytokine IL-1 $\beta$ production. Neurobiology of Aging. 2004. 25: 431-439. https://doi.org/10.1016/S0197-4580(03)00126-X
  10. Kolak U, Boga M, Urugak EA, Ulubelen A. Constituents of Plantago Major subsp. intermedia with antioxidant and anticholinesterase capacities. Turkish Journal of Chemistry. 2011. 35:637-645.
  11. Nanjo F, Goto K, Seto R, Suzuki M. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Biology and Medicine. 1996. 21: 895-902. https://doi.org/10.1016/0891-5849(96)00237-7
  12. Nunez Guillen ME, da Silva Emim JA, Souccar C, Lapa AJ. Analgesic and anti-inflammatory activities of the aqueous extract of Plantago Major L. Pharmaceutical Biology. 1997. 35: 99-104. https://doi.org/10.1076/phbi.35.2.99.13288
  13. Matsumoto Y, Ohmori K, Fujiwara M. Immune regulation by brain cells in the central nervous system: microglia but not astrocytes present myelin basic protein to encephalitogenic T cells under in vivo mimicking conditions. Immunology. 1992. 76: 209-216.
  14. Merrill JE, Benveniste EN. Cytokines in inflammatory brain lesions: helpful and harmful. Trends in Neurosciences. 1996. 19: 331-338. https://doi.org/10.1016/0166-2236(96)10047-3
  15. Park TK, Koppula S, Kim MS, Jung SH, Kang H. Anti-neuroinflammatory effects of Houttuynia cordata extract on LPSstimulated BV-2 microglia. Tropical Journal of Pharmaceutical Research. 2013. 12: 523-528.
  16. Perry RT, Collins JS, Wiener H, Acton R, Go RC. The role of TNF and its receptors in Alzheimer's disease. Neurobiology of Aging. 2001. 22: 873-883. https://doi.org/10.1016/S0197-4580(01)00291-3
  17. Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology. 2009. 7: 65-74. https://doi.org/10.2174/157015909787602823
  18. Velasco-Lezama R, Tapia-Aguilar R, Roman-Ramos R, Vega-Avila E, Perez-Gutierrez MS. Effect of Plantago Major on cell proliferation in vitro. Journal of Ethnopharmacology. 2006. 103:36-42. https://doi.org/10.1016/j.jep.2005.05.050