Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Inflammation inhibitory effect of water extract from pumpkin's tendril

호박 덩굴손 물 추출물의 염증제어 활성

  • Jeong, Ha-Na (Department of and BK21 Plus Program, Chonnam National University) ;
  • Choi, Ju-Hee (Laboratory animal Medicine, College of Veterinary Medicine and BK21 PLUS Project Team, Chonnam National University) ;
  • Lee, Ha-Nul (Laboratory animal Medicine, College of Veterinary Medicine and BK21 PLUS Project Team, Chonnam National University) ;
  • Lee, So-Hyeon (Department of and BK21 Plus Program, Chonnam National University) ;
  • Cho, Soon-Chang (NaturePureKor) ;
  • Park, Jong-Hwan (Laboratory animal Medicine, College of Veterinary Medicine and BK21 PLUS Project Team, Chonnam National University) ;
  • Kim, Young-Min (Department of and BK21 Plus Program, Chonnam National University)
  • 정하나 (전남대학교 식품공학과 및 BK21 Plus 프로그램) ;
  • 최주희 (전남대학교 수의학과 실험동물학 교실) ;
  • 이하늘 (전남대학교 수의학과 실험동물학 교실) ;
  • 이소현 (전남대학교 식품공학과 및 BK21 Plus 프로그램) ;
  • 조순장 (네이처퓨어코리아) ;
  • 박종환 (전남대학교 수의학과 실험동물학 교실) ;
  • 김영민 (전남대학교 식품공학과 및 BK21 Plus 프로그램)
  • Received : 2017.11.10
  • Accepted : 2017.12.18
  • Published : 2017.12.30
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Abstract

Pumpkin has long been used as traditional health materials in oriental medicine, pharmacy, medicine, and food industries in many countries. In this study, the water extract and two active components from tendril of young C. moschata Duch. were investigated on inflammation inhibitory activity. The water extract of young C. moschata Duch. showed high cell viability over 95% and it decreased the production of interlukin 6 (IL-6) and tumor necrosis factor (TNF-${\alpha}$) in the capacity of mouse bone marrow-derived macrophages (BMDM) upon lipopolysaccharide (LPS) stimulation. Also, isolated fraction (B4) suppressed the secretion of IL-6 and TNF-${\alpha}$. Among the domestically cultivated pumpkins, B4 fraction contained in the tendril part of them and it comprised of the order of tendril from Cucurbita pepo var. cylindrica, old of C. moschata Duch., and young of C. moschata Duch. These results suggest that water extracts of C. moschata Duch. and purified active compound, rutin, show anti-inflammation activity by suppression of the secretion of IL-6 and TNF-${\alpha}$. It can be applicable as pharmaceutical materials.

애호박의 부위 중 덩굴손 유래의 열수 추출물에 대한 세포 독성 및 염증제어활성 대하여 조사하였다. 애호박 덩굴손의 열수에 대한 세포의 독성에 미치는 영향을 조사한 결과 0.313-2.5 mg/mL의 농도일 때 물 추출물은 세포의 생장에 영향을 미치지 않아 세포에 대한 독성이 없다고 판단되었다. BMDMs를 배양한 세포에 호박덩굴손 열수 추출물과 LPS와 함께 처리한 결과 염증성 사이토카인인 IL-6 및 TNF-${\alpha}$의 농도 의존적으로 감소함을 확인하였다. 호박 덩굴손 열수 추출물로부터 단리 정제된 rutin에 의한 염증성 사이토카인의 분비가 억제되었다. 국내에서 재배되고 있는 호박의 부위별로 조사해본 결과 호박부위 중 유일하게 덩굴손부분에서 염증억제 활성을 보였다. 쥬키니 호박, 애호박 및 늙은 호박의 덩굴손에서 유효성분인 rutin을 함유하고 있었고 향후 염증 억제 소재로서의 활용가능성을 시사하였다.

Keywords

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