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Comparison of in vitro ruminal fermentation between different originated corn grains

  • Kim, Do Hyung (Department of Animal Science, Gyeongbuk Provincial College) ;
  • Park, Ha Young (Department of Pathology, Busan Paik Hospital, Inje University College of Medicine) ;
  • Choi, Chang Weon (Department of Animal Resources, Daegu University)
  • Received : 2017.07.20
  • Accepted : 2017.10.31
  • Published : 2017.12.31

Abstract

This study was conducted to compare in vitro rumen fermentation characteristics among corn grains imported from America, Brazil, Argentina and Ukraine A and Ukraine B. Two Holstein steers, each surgically fitted with a ruminal cannula, consuming total mixed ration were used as rumen fluid donors. In vitro rumen fermentation experiments were performed in a completely random design which included a control (no corn) and treatments with 3.0 g of corn from different geographical origins, i.e., America, Brazil, Argentina, and Ukraine A and Ukraine B, respectively. Ruminal pH, ammonia-N, volatile fatty acid (VFA) and total gas production were measured at 0, 1, 3, 6, 12, 24 and 48 h post-incubation, respectively. No differences (p > 0.05) in mean ruminal pH appeared among the treated groups, however, ruminal pH patterns differed; i.e. corn treated groups had dramatically lower pH compared with control during the entire incubation period. Similarly, no different patterns between the groups in ammonia-N (p > 0.05) appeared until 6 h post-incubation. Unexpectedly, higher ammonia-N concentration for control than that for the corn treated groups appeared after 12 h post-incubation despite that for all groups increased. Total VFA was similar between the groups until 6 h post-incubation, but VFA after 12 h post-incubation was different (p < 0.05), i.e. VFA for corn from Argentina, Ukraine A, Ukraine B, and Brazil were comparatively higher than for America. Overall, data in this study showed that the corns of different origins may have different feed values to ruminants despite having similar chemical compositions.

Keywords

References

  1. AFRASY. 2016. Agriculture, food and rural affairs statistics yearbook. Director of Information & Statistics & Policy Division, Ministry of Agriculture, Food and Rural Affairs, Korea. 375 pp.
  2. Chaney AL, Markbach EP. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry 8:130-137.
  3. Choi CW. 2015. Effects of CNCPS fraction-enriched proteins on ruminal fermentation and plasma metabolites in Holstein steers fed TMR containing low protein. CNU Journal of Agricultural Science 42:237-244.
  4. Hristov AN, McAllister TA, Cheng KJ. 2000. Intraruminal supplementation with increasing levels of exogenous polysaccharide-degrading enzymes: Effects on nutrient digestion in cattle fed a barley grain diet. Journal of Animal Science 78:477-487. https://doi.org/10.2527/2000.782477x
  5. Lee SM, Kang TW, Lee SJ, Ok JU, Moon YH, Lee SS. 2006. Studies on in situ and in vitro degradabilities, microbial growth and gas production of rice, barley and corn. Journal of Animal Science and Technology 48:699-708. [in Korean] https://doi.org/10.5187/JAST.2006.48.5.699
  6. Lee SU, Jo JH, Park SK, Choi CW, Jeong J, Chung KY, Chang SS, Li XZ, Choi SH. 2016. Ruminal microbial responses in fermentation characteristics and dry matter degradability to TDN level of total mixed ration. Korean Journal of Agricultural Science 43:80-86. https://doi.org/10.7744/kjoas.20160010
  7. Ma T, Deng KD, Jiang CG, Tu Y, Zhang NF, Liu J, Zhao YG, Diao QY. 2013. The relationship between microbial N synthesis and urinary excretion of purine derivatives in Dorper $\times$ thin-tailed Han crossbred sheep. Small Ruminant Research 112:49-55. https://doi.org/10.1016/j.smallrumres.2012.09.003
  8. McDougall EI. 1948. Studies on ruminant saliva. The composition and output of sheep's saliva. Biochemistry Journal 43:99-109. https://doi.org/10.1042/bj0430099
  9. Moon HS. 2014. Comparison of quality analysis of imported corn origins. FIRI research trends 67:71-76. Korean Feed Association. Accessed in http://www.kofeed.org/bbs/selectBoardArticle.do. [in Korean]
  10. Mould FL, Orskov ER, Mann SO. 1984. Associative effects of mixed feeds. I. Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages. Animal Feed Science and Technology 10:15-30.
  11. Orskov ER, McDonald I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science 92:499-503. https://doi.org/10.1017/S0021859600063048
  12. Pina DS, Valadares Filho SC, Tedeschi LO, Barbosa AM, Valadares RFD. 2009. Influence of different levels of concentrate and ruminally undegraded protein on digestive variables in beef heifers. Journal of Animal Science 87:1058-1067. https://doi.org/10.2527/jas.2008-1069
  13. Ramos S, Tejido ML, Martinez ME, Ranilla MJ, Carro MD. 2009. Microbial protein synthesis, ruminal digestion, microbial populations, and nitrogen balance in sheep fed diets varying in forage-to-concentrate ratio and type of forage. Journal of Animal Science 87:2924-2934. https://doi.org/10.2527/jas.2009-1938
  14. Reynal SM, Broderick GA. 2005. Effect of dietary level of rumen-degraded protein on production and nitrogen metabolism in lactating dairy cows. Journal of Dairy Science 88:4045-4064. https://doi.org/10.3168/jds.S0022-0302(05)73090-3
  15. Sallam SMA, Nasser MEA, El-Waziry AM, Bueno ICS, Abdalla AL. 2007. Use of an in vitro rumen gas production technique to evaluate some ruminant feedstuffs. Journal of Applied Sciences Research 3:34-41.
  16. SAS. 2002. Sas User's Guide. Statistics. Version 9.1. SAS Institute. Inc. Cary, NC, USA.
  17. Siverson A. 2013. Effects of corn processing and dietary wet corn gluten feed on newly received and growing cattle. Master Thesis. Kansas State University, Manhattan, Kansas.
  18. Theodorou MK, Williams BA, Dhanoa MS, McAllan AB, France J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48:185-197. https://doi.org/10.1016/0377-8401(94)90171-6
  19. Valadares RFD, Broderick GA, Valadares Filho SC, Clayton MK. 1999. Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives. Journal of Dairy Science 82:2686-2696. https://doi.org/10.3168/jds.S0022-0302(99)75525-6
  20. Van Soest PJ, Robertson JB, Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

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