Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-atopic Effect of Zizyphus jujuba var. spinosa Composite oil(ZjJ-C_oil) and Aralia cordata var. continentalis Composite oil(ARC-C_oil) in DNCB-induced Atopic Dermatitis-like Skin Lesions NC/Nga Mice

천연물 유래 산조인 복합오일 (Zizyphus jujuba var. spinosa Composite oil, ZjJ-C_oil) 및 독활 복합오일 (Aralia cordata var. continentalis Composite oil, ARC-C_oil)의 DNCB로 유도된 NC/Nga 생쥐의 아토피에 미치는 효과 연구

  • Kwak, Jin-young (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon Univ) ;
  • Choir, Hee-Jeong (Graduate School of Intergrated Medicine, Cha University) ;
  • Park, Jung-mi (Cheonan oriental hospital of Daejeon Univ. Wellness spa Clinical Cente) ;
  • Park, Jung-hwan (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon Univ) ;
  • Kho, Young-mee (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon Univ) ;
  • Jang, Tae-soo (Institutes of Green Bio Science Technology, Seoul National University) ;
  • Ahn, Teakwon (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon Univ)
  • 곽진영 (대전대학교 한의과대학 사상체질의학과) ;
  • 최희정 (차의과대학교 통합의학대학원) ;
  • 박정미 (대전대전대학교 천안한방병원 웰니스 스파 임상센터) ;
  • 박정환 (대전대학교 한의과대학 사상체질의학과) ;
  • 고영미 (대전대학교 한의과대학 사상체질의학과) ;
  • 장태수 (서울대학교 그린바이오과학기술연구원) ;
  • 안택원 (대전대학교 한의과대학 사상체질의학과)
  • Received : 2017.12.18
  • Accepted : 2017.12.21
  • Published : 2017.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study is to investigate the effect of Zizyphus jujuba var. spinosa Composite oil(ZjJ-C_oil) and Aralia cordata var. continentalis Composite oil(ARC-C_oil) to NC/Nga mice induced in Atopic dermatitis-like skin lesions by DNCB. NC/Nga mice which have been induced to Atopic dermatitis-like skin lesions by DNCB are divided into 4 groups, the first is the mice which have been spread with izyphus jujuba var. spinosa Composite oil(ZjJ-C_oil), the second is the mice which have been spread with Aralia cordata var. continentalis Composite oil(ARC-C_oil), the third is which have been spread with dexamethasone (Dexa.) 0.5% on their Atopic lesion, the last is the control group. Then We analyzed skin clinical score, blood sample of each group of measure state of the dorsal skin, the number of immunocytes, and resect the skin lesion to anlayze the state of cells. There are meaningful results of measuring the number of IgE, IL-4, IL-13, $IFN-{\gamma}$, IL-5, the total cells in ALN, dorsal skin, CD4+ Th, CD11b+/Gr-1+ in PBMCs, CD4+ Th, B220+/CD23+ in ALN, $CD4^+$, $CD8^+$ Th in dorsal skin, the level of COX-2, $TNF-{\alpha}$, $TGF-{\beta}1$, IL-4, IL-13 mRNA, the state of the skin lesion and cells in the group with ZjJ-C_oil, ARC-C_oil cream in comprarison with the control group.

Keywords

References

  1. Journal of asthma,allergy and chinical immunology, 40 million allergies. Seoul: Medical history corporation of medical science. 2005:1-22, 233-239(Korean).
  2. SP Yoon, BS Kim, JH Ree, SC Lee, YK Kim. The environment and lifestyle of atopic dermatitis patients. Korean J. Dermatol. 1999;37:983-991(Korean).
  3. HS Ahn, Hongchangui pediatrics. 9th edition. Seoul. Miraen, p.1142, 2010.(Korean).
  4. HJ Lee, CY P, JH Lee, KH Lee: The Antipruritic Effect of Topical Doxepin Cream in Patients with Atopic Dermatitis, Korean journal of dermatology, 2006;44(3):309-14.
  5. JD Guin. Complications of topical hydrocortisone. J Am Acad Dematol. 4:417-422, 1981. https://doi.org/10.1016/S0190-9622(81)70040-9
  6. H Sugano. Psychology and Biology of Fragrance I. Chapman and Hall. 1990.
  7. Okada M, Hirasawa Y, Yoshijima K et al. : Effect of clobetasol propionate, a corticosteroid on hapten-induced dermatitis in SPF NC/Nga mouse, Pharmacometrics, 59:135-139, 2000.
  8. Lee JM. Dongeisusebowon. Seoul: Daesungmoonhwasa. 1997;1:5, 4:1,10,29-32(Korean).
  9. SK Myungm, A Survey Study about Eating Habits, Living Environment and Sasang Constitution of Children with Atopic Dermatitis, The Graduate School of Education at Incheon National University, a Master's degree, 2014;1-95(Korean).
  10. National College of Oriental Medicine Collaborative Textbook Compilation Committee, Herbal medicine. Seoul: YeongLimSa. 2010; 323(Korean).
  11. JH Lee, JY Baek, HS Jeong, SD Park., A clinical study of Sanjoincho - induced injection on insomia patients, The Korean Society of Oriental Neuropsychiatry, 2000;11(2):79-86.
  12. SK Lee, DJ Joe, HJ Jang, K Hyun, Journal of Naturopathy, Antioxidant and Anti-inflammatory Activities of Ethanol Extracts from Aralia continentalis Kitagawa, 2015;4(1):10-14.
  13. SI Noh, SD Kim, SC Park, BY Seo, SR Yeom, YD Kwon, et al.,Mechanism of Anti-inflammatory Effect of Peucedanum japonicum Thunb, Journal of Oriental Rehabilitation Medicine, 2005;15(2):31-43.
  14. Abramovits W. Atopic dermatitis. J Am Acad Dermatol. 2005;53(Suppl):S98-104.
  15. Valladeau J, Saelan S. Cutaneous dendritic cells. Semin Immunol. 2005;17:273-283. https://doi.org/10.1016/j.smim.2005.05.009
  16. Beltrani VS. The clinical spectrum of atopic. 21(1):80-90. 2013.
  17. Richard A. Goldsby, Thomas J. Kindt, Barbara A. Osborne, Janis Kuby. KUBY Immunology 5th.. World Science,. 2006;104-5, 310, 327-30.
  18. YM Park,. Advances In the Pathophysiology of Atopic Dermatitis. The Korean Academy of Pediatric Allergy and Respiratory Disease, 2006;16(3):190.(Korean).
  19. Abul K. Abbas, Andrew H. Lichtman. Basic immunology 3th. Epublic,. 2009;123-4.
  20. TY Kim, Therapeutic effect of IFN-${\gamma}$ in refractory atopic dermatitis. The Korean Dermatological Association, 2000;38(10):55.(Korean).
  21. Ying, S, Taborda-Barata, L, Meng, Q, Humbert, M, Kay, AB: The kinetics of allergen-induced transcription of messenger RNA for monocyte chemotactic protein-3 and RANTES in the skin of human atopic subjects:relationship to eosinophil, T cell, and macrophage recruitment. J Exp Med, 181:2153-2159, 1995. https://doi.org/10.1084/jem.181.6.2153
  22. Bronte V, Wang M, Overwijk WW, et al.:Apoptotic death of CD8+T lymphocytes after immunization: induction of a suppressive population of Mac-1+/Gr-1+ cells. J Immunol, 161(10):5313-5320, 1998.
  23. Ryouji Yagi, Haruko Nagai, Yutaka Iigo, Toshihiro Akimoto, Takao Arai and Masato Kubo: Development of Atopic Dermatitis-Like Skin Lesions in STAT6-Deficient NC/Nga mouse. The Journal of Immunology. 168:2020-2027. 2002. https://doi.org/10.4049/jimmunol.168.4.2020
  24. Young MR, Wright MA, Matthews JP, Malik I, Prechel M: Suppression of T cell proliferation by tumor-induced granulocyte macrophage progenitorcells producing transforming growth factor-b and nitric oxide. J Immunol, 156(5):1916-1922, 1996.
  25. Rosenwasser, L.J., Busse, W.W., Lizambri, R.G., Olejnik, T.A., Totoritis, M.C : Allergic asthma and an anti-CD23+ mAb (IDEC-152): results of a phase I, single-dose, dose-escalating clinical trial. J Allergy Clin Immunnol. 112(3):563-570. 2003. https://doi.org/10.1016/S0091-6749(03)01861-X
  26. Heijden FL, Joost N, Katwijk M, Bos JD, Kapsenberg ML. Serum-IgE-facilitated allergen presentation in atopic disease. J Immunol. 1993;150(8):3643-50.
  27. The Korean Academy of Asthma, Allergy and Clinical Immunology. Asthma and Allergic disease. Seoul: Koonja Publshing Co. 2002:13-116.
  28. Komai-Koma, M and PC Wilkinson. TGF-beta stimulates but IFN-gamma inhibits growth-related activation of locomotion of human B cells. The Journal of Immunology, 1997;(158):3125-3129.