Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Anti-Inflammatory Activities of Crude Extract and Solvent Fractions of Allium hookeri

삼채의 조추출물과 유기용매 분획물에 대한 항산화 및 항염증 효과

  • Lee, Yong-Bum (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Ham, Young-Min (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Yoon, Seon-A (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Oh, Dae-Ju (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Song, Sang-Mok (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Hong, In-Choel (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Lee, Si Taek (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Hyun, Ho Bong (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Kim, Chang-Suk (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP))
  • 이용범 ((재)제주테크노파크 생물종다양성연구소) ;
  • 함영민 ((재)제주테크노파크 생물종다양성연구소) ;
  • 윤선아 ((재)제주테크노파크 생물종다양성연구소) ;
  • 오대주 ((재)제주테크노파크 생물종다양성연구소) ;
  • 송상목 ((재)제주테크노파크 생물종다양성연구소) ;
  • 홍인철 ((재)제주테크노파크 생물종다양성연구소) ;
  • 이시택 ((재)제주테크노파크 생물종다양성연구소) ;
  • 현호봉 ((재)제주테크노파크 생물종다양성연구소) ;
  • 김창숙 ((재)제주테크노파크 생물종다양성연구소) ;
  • 윤원종 ((재)제주테크노파크 생물종다양성연구소)
  • Received : 2016.09.23
  • Accepted : 2016.12.19
  • Published : 2017.01.31
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Abstract

This study describes the preliminary evaluation of antioxidant and anti-inflammatory activities of Allium hookeri. A. hookeri was extracted using crude extract and then fractionated sequentially with n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, we first examined the inhibitory effect of A. hookeri extracts on production of oxidant stresses (2,2-diphenyl-1-picrylhydrazyl, xanthine oxidase, and superoxide). In addition, we examined the inhibitory effects of A. hookeri on production of pro-inflammatory factors (nitric oxide, prostaglandin $E_2$, inducible nitric oxide synthase, and cyclooxygenase-2) in murine macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Of the sequential solvent fractions of A. hookeri, EtOAc fractions showed decreased production of oxidant stresses, and $CH_2Cl_2$ and EtOAc fractions of A. hookeri inhibited production of pro-inflammatory factors. EtOAc fraction inhibited production of pro-inflammatory cytokines (interleukin-6 and -$1{\beta}$). These results suggest that A. hookeri has significant effects on oxidant stresses and pro-inflammatory factors and is a possible antioxidant and anti-inflammatory therapeutic and preventive material.

본 연구는 삼채의 조추출물과 유기용매 분획물들을 가지고 DPPH 라디칼 소거 활성에 의한 항산화 활성 검색 결과 디클로로메탄($CH_2Cl_2$) 분획물과 에틸아세테이트(EtOAc) 분획물에서 라디칼 소거 활성을 나타냈으며, xanthine oxidase 억제 효과는 DPPH 활성 라디칼 소거 활성에서 제일 뛰어났던 에틸아세테이트 분획물에서, superoxide 소거 활성은 헥산(n-hexane) 분획물에서 활성이 나타났다. RAW 264.7 세포에 lipopolysaccharide로 자극을 주고 삼채 주정 추출물 및 유기용매 분획물들을 처리하여 확인해본 결과, 조추출물과 물 분획물을 제외한 나머지 유기용매 분획물에서 염증유발 인자(NO, $PGE_2$, iNOS, COX-2, IL-6 및 $IL-1{\beta}$) 생성억제 효과가 나타났으며, 그중 디클로로메탄 분획물과 에틸아세테이트 분획물에서 억제 효과를 확인할 수 있었다. 본 실험 결과 삼채 조추출물과 유기용매 분획물에서 항산화 효과 및 염증 유발 인자의 생성 억제 효과가 나타났으며, 이러한 결과 삼채에서 유효성분 추출을 통한 항산화, 항염증 물질의 연구 또는 예방하거나 치료할 수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 생각한다.

Keywords

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