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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Vitamin C enhances the expression of IL17 in a Jmjd2-dependent manner

  • Song, Mi Hye (Department of Pathology, Hallym University College of Medicine) ;
  • Nair, Varun Sasidharan (Department of Pathology, Hallym University College of Medicine) ;
  • Oh, Kwon Ik (Department of Pathology, Hallym University College of Medicine)
  • Received : 2016.11.21
  • Accepted : 2016.12.08
  • Published : 2017.01.31
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Abstract

Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in $CD4^+Foxp3^+$ regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interestingly, the upregulation of IL17 was not accompanied by DNA demethylation in Il17 promoter and was independent of Tet enzymes. Rather, vitamin C reduced the trimethylation of histone H3 lysine 9 (H3K9me3) in the regulatory elements of the Il17 locus, and the effects of vitamin C were abrogated by knockdown of jumonji-C domain-containing protein 2 (jmjd2). These results suggest that vitamin C can affect the expression of IL17 by modulating the histone demethylase activity.

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References

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