Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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Analysis of total oral microorganisms in saliva using real-time PCR and colony forming unit

Real-time PCR과 Colony forming unit법을 이용한 타액 내 2종의 구강미생물 총량분석

  • Yoo, Su-Min (Departments of Dental Hygiene, Kyungdong University) ;
  • Jeong, Seong-Kug (Departments of Preventive Dentistry, School of Dentistry, Chosun University) ;
  • Yoo, Hyun-Jun (Departments of Preventive Dentistry, School of Dentistry, Dankuk University) ;
  • Jang, Jong-Hwa (Departments of Dental Hygiene, Hanseo University)
  • 유수민 (경동대학교 치위생학과) ;
  • 정성국 (조선대학교 치과대학 예방치과교실) ;
  • 유현준 (단국대학교 치과대학 예방치과교실) ;
  • 장종화 (한서대학교 치위생학과)
  • Received : 2016.12.30
  • Accepted : 2017.01.31
  • Published : 2017.02.28
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Abstract

Objectives: The purpose of this study was to compare colony forming unit (CFU) method and multiplex real-time polymerase chain reaction (MRT-PCR) method for accurate quantitative analysis of bacteria. Methods: We compared the CFU method and the MRT-PCR method, which are still used in Korea, for Prevotella intermedius (P. intermedius), a periodontal disease pathogen selected by MRT-PCR, and Streptococcus mutans (S. mutans), a dental caries causative organism. The subjects of this study were 30 patients who visited the C dental hospital. Results: Total microorganisms in MRT-PCR method were significantly higher in both types of bacteria (p<0.05), since DNA of dead bacteria was also analyzed. This was because the periodontal dise(-) anaerobes, and even dead bacteria contain large amounts of toxic substances called LPS in the extracellular membrane, and fimbriae and pili, which are motility structures, still remain as a strong toxic substance in periodontal tissue. Conclusions: Therefore, in terms of the total amount of bacteria found, the MRT-PCR method will be a useful technique for searching all the bacteria in the oral cavity including live bacteria, as well as sterilization.

Keywords

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