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반응표면분석법을 이용한 꾸지뽕의 블랜칭 처리 및 건조 조건 최적화

Optimization of drying conditions of Cudrania tricuspidata using response surface methodology

  • Park, Jong-Jin (School of Food Science and Technology, Kyungpook National University) ;
  • Park, Dae-Hee (School of Food Science and Technology, Kyungpook National University) ;
  • Jung, Gayoung (Wellness R&D Center, Univera Inc.) ;
  • Shin, Eunju (Wellness R&D Center, Univera Inc.) ;
  • Do, Seon-Gil (Wellness R&D Center, Univera Inc.) ;
  • Lee, Wonyoung (School of Food Science and Technology, Kyungpook National University)
  • 투고 : 2017.02.01
  • 심사 : 2017.02.16
  • 발행 : 2017.02.28

초록

꾸지뽕 열매는 미생물에 의한 오염과 물러짐으로 저장기간이 짧고 유통이 어렵다는 단점이 있다. 농산물에 적용할 수 있는 선도 유지 및 가공법은 여러 방법이 있지만 본 연구에서는 실질적으로 농민들이 적용할 수 있는 블랜칭 처리방법과 열풍 건조법에 대해 연구하였다. 먼저 블랜칭 처리 시간별로 꾸지뽕의 생균수를 측정하여 블랜칭 처리 시간이 증가할수록 미생물이 점차적으로 감소하는 것을 확인할 수 있었다. 그리고 블랜칭 처리 시간 및 건조 조건에 따른 건조 추세를 비교한 결과, $40^{\circ}C$에서 건조 시 무처리구 보다 블랜칭 처리구가 더 빠른 수분 감소 효과를 나타내었다. 하지만 건조 온도가 증가할수록 무처리구와 블랜칭 처리구 간의 건조 추세 차이는 점점 줄어드는 것을 확인하였다. 최종적으로 블랜칭 및 열풍건조의 최적 조건을 도출하기 위해 반응표면 분석법을 이용하였다. 조건에 따른 total polyphenol content, total flavonoid content, DPPH radical scavenging activity, 색차(${\Delta}E$)의 예측값은 각각 8.62 mg GAE/g, 56.65 mg RE/g, 40.26%, 11.69의 수치를 나타냈다. 최적 범위 내 임의의 조건 즉, 240 sec 블랜칭 처리 시간, $60^{\circ}C$ 건조 온도, 24 h 건조 시간에서 실험값은 10.06 mg GAE/g, 49 mg RE/g. 44.99%, 10.53을 나타냈으며 예측값과 실험값은 유사한 값을 보였다.

This study was conducted to obtain the optimal conditions of hot air drying for Cudrania tricuspidata by response surface methodology (RSM). The independent variables were blanching time (60, 120, 240 sec), drying temperature (40, 60, $80^{\circ}C$) and drying time (12, 24, 36 h). The dependant variables were total polyphenol content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DPPH), and color difference (${\Delta}E$). Viable cell colony was counted according to changes of blanching time. It was confirmed that microorganisms gradually decreased with increasing blanching time. From RSM results, the predicted values of TPC, TFC, DPPH, and ${\Delta}E$ were 8.62 mg GAE/g, 56.65 mg RE/g, 40.26% and 11.69, respectively. Experimental values within the optimal range (240 sec, blanching time; $60^{\circ}C$, drying temperature; 24 h, drying time) were 10.06 mg GAE/g, 49 mg RE/g, 44.99% and 10.53, respectively. The predicted values were similar to the experimental values. Comparing drying tendency according to changes of blanching time, moisture reduction was bigger in the blanched sample than that in control at $40^{\circ}C$. However, the differences between blanched and control decreased with increase of drying temperature. Viable cell gradually decreased as increasing blanching time.

키워드

참고문헌

  1. Lee CB (1985) Dehanshikmuldogam (A field guide to Korean plants). Hyangmoonsha, Seoul, Korea, p 285
  2. Kangjoshinewhakwon : Jungyakdesajon (Great dictionary of Chinese medicine) (1985) 2nd ed, Sohakkyan, Shanghai, China, p 2383
  3. Park JH, Lee KW, Sung KS, Kim SS, Cho KD, Lee BH, Han CK (2012) Effect of diets with mulberry leaf and Cudrania tricuspidata leaf powder supplements on blood glucose-related biomarkers in streptozotocininduced diabetic rats. J Korean Soc Food Sci Nutr, 41, 766-773 https://doi.org/10.3746/jkfn.2012.41.6.766
  4. Kang YK, Lee EA, Park HR (2012) Neuroprotective effect according to reactive oxygen species scavenging activity from extracts of Cudrania tricuspidata leaves. Korean J Food Cook Sci, 28, 821-828 https://doi.org/10.9724/kfcs.2012.28.6.821
  5. Shin HS, Jeong HL, Hwang DB, Kim DU (2014) Cudrania tricuspidata root extract as whitening and antiwrinkle cosmetic agent. Korean Chem Eng Res, 52, 701-705 https://doi.org/10.9713/kcer.2014.52.6.701
  6. Jung GT, Ju IO, Choi SR, You DH, Noh JJ (2013) Food nutritional characteristics of fruit of Cudrania tricuspidata in its various maturation stages. Korean J Food Preserv, 20, 330-335 https://doi.org/10.11002/kjfp.2013.20.3.330
  7. Lee HK, Kim YS (2015) Comparison of proximate composition of Curdrania tricuspidata Bureau fruit. J Agric Life Sci, 46, 22-26
  8. Joo HY, Lim KT (2009) Protective effect of glycoprotein isolated from Cudrania tricuspidata on liver in $CCl_4$-treated A/J mice. Korean J Food Sci Technol, 41, 93-99
  9. Oh PS, Lee HJ, Lim KT (2009) Inhibitory effect of glycoprotein isolated from Cudrania tricuspidata Bureau on histamine release and COX-2 activity in RBL-2H3 cells. Korean J Food Sci Technol, 41, 405-412
  10. Youn KS, Kim JW (2012) Antioxidant and angiotensin converting enzyme I inhibitory activities of extracts from mulberry (Cudrania tricuspidata) fruit subjected to different drying methods. J Korean Soc Food Sci Nutr, 41, 1388-1394 https://doi.org/10.3746/jkfn.2012.41.10.1388
  11. Seo MJ, Kang BW, Park JU, Kim MJ, Lee HH, Kim NH, Kim KH, Rhu EJ, Jeong YK (2013) Effect of fermented Cudrania tricuspidata fruit extracts on the generation of the cytokines in mouse spleen cells. J Life Sci, 23, 682-688 https://doi.org/10.5352/JLS.2013.23.5.682
  12. Kang DH, Kim JW, Youn KS (2011) Antioxidant activities of extracts from femented mulberry (Cudrania tricuspodata) fruit, and inhibitory actions on elastase and tyrosinase. Korean J Food Preserv, 18, 236-243 https://doi.org/10.11002/kjfp.2011.18.2.236
  13. Choi SR, You DH, Kim JY, Park CB, Kim DH, Ryu J (2009) Antioxidant activity of methanol extracts from Curdrania tricuspidata Bureau according to harvesting parts and time. Korean J Medicinal Crop Sci, 17, 115-120
  14. Kim HJ (2012) Antioxidant and antibacterial activity of extracts from Cudrania tricuspidata Bureau. MS Thesis, Chungnam National University, Korea, p 38-45
  15. Kang JS, Cho HR, Han JS, Hur SH (2003) Hot water dipping treatment to improve storage quality of green red pepper. Korean J Food Preserv, 10, 261-266
  16. Lee YJ, Lee HO, Kim JY, Kwon KH, Cha HS, Kim BS (2011) Quality characteristics of frozen Doraji (Platycodon grandiflorum) according to various blanching treatment conditions. Korean J Food Preserv, 18, 661-668 https://doi.org/10.11002/kjfp.2011.18.5.661
  17. Lee MK, Kim SH, Ham SS, Lee SY, Chung CK, Kang IJ, Oh DH (2000) The effect of far infrared ray-vacuum drying on the quality changes of Pimpinella bracycarpa. J Korean Soc Food Sci Nutr, 29, 561-567
  18. Mujurndar AS, Menon AS (1995) Drying of solids: principles, classification and selection of dryers. In: Handbook of Industrial Drying, Mujurndar AS (Editor), Marcel dekker, New York, NY, USA, p 1-39
  19. Jung YK, Jang MY, Hwang IG, Yoo SM, Min SG, Jo YJ, Chun JY, Choi MJ (2015) Combination effect of various freezing and thawing techniques on quality and nutritional attributes of onions. J Korean Soc Food Sci Nutr, 44, 1492-1503 https://doi.org/10.3746/jkfn.2015.44.10.1492
  20. Chung HS, Seong JH, Lee YG, Kim HS, Lee JB, Youn KS (2009) Browning and moisture sorption characteristics of Rubus coreanus prepared by different drying methods. Korean J Food Preserv, 16, 797-803
  21. Teng H, Lee WY (2015) Drying kinetics and optimization for thin-layer drying processes of raspberries (Rubus coreanus Miq.) using statistical models and response surface methodology. Korean J Food Preserv, 22, 1-11 https://doi.org/10.11002/kjfp.2015.22.1.1
  22. Bhattarai S, Oh JH, Choi YS, Oh KC, Euh SH, Kim DH (2012) Microwave drying of sawdust for pellet production: kinetic study under batch mode. J Biosystems Eng, 37, 385-397 https://doi.org/10.5307/JBE.2012.37.6.385
  23. Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 299, 152-178
  24. Teng H, Ghafoor K, Choi YH (2009) Optimization of microwave-assisted extraction of active components from Chinese quince using response surface methodology. J Korean Soc Appl Biol Chem, 52, 694-701 https://doi.org/10.3839/jksabc.2009.115
  25. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  26. Kim JW, Youn KS (2014) Phytochemical compounds and quality characteristics of Aster scaber Thunb. in response to blanching conditions and treatment with solutes. Korean J Food Preserv, 21, 694-701 https://doi.org/10.11002/kjfp.2014.21.5.694
  27. Lim JH, Choi JH, Hong SI, Jeong MC, Kim DM (2005) Mild heat treatments for quality improvement of fresh-cut potatoes. Korean J Food Preserv, 12, 552-557
  28. Chung SK, Shin JC, Choi JU (1992) The blanching effects on the drying rates and the color of hot red pepper. J Korean Soc Food Nutr, 21, 64-69
  29. Pirone BN, De Michelis A, Salvatori DM (2014) Pretreatments effect in drying behaviour and colour of mature and immature 'Napolitana' sweet cherries. Food Bioprocess Technol, 7, 1640-1655
  30. MAZZA G (1983) Dehydration of carrots: effects of pre-drying treatments on moisture transport and product quality. Int J Food Sci Technol, 18, 113-123
  31. Borges SV, Mancini MC, Correa JLG, Leite JB (2011) Drying kinetics of bananas by natural convection: Influence of temperature, shape, blanching and cultivar. Sci agrotec, 35, 368-376 https://doi.org/10.1590/S1413-70542011000200019
  32. Demirel D, Turhan M (2003) Air-drying behavior of Dwarf Cavendish and Gros Michel banana slices. J Food Eng, 59, 1-11 https://doi.org/10.1016/S0260-8774(02)00423-5
  33. Tomas-Barberan FA, Espin JC (2001) Phenolic compounds and related enzymes as determinants of quality of fruits and vegetables. J Sci Food Agric, 81, 853-876 https://doi.org/10.1002/jsfa.885
  34. Ghasemzadeh A, Ghasemzadeh N (2001) Flavonoids and phenolic acids: Role and biochemical activity in plants and human. J Med Plants Res, 5, 6697-6703
  35. Hwang IG, Shin YJ, Lee S, Lee J, Yoo SM (2012) Effect of different cooking methods on the antioxidant properties of red pepper (Capsicum annuum L.). Prev Nutr Food Sci, 17, 286-292 https://doi.org/10.3746/pnf.2012.17.4.286
  36. Dewanto V, Wu X, Adom KK, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem, 50, 3010-3014 https://doi.org/10.1021/jf0115589
  37. Jin CR, Cho CH, Nam TG, Cho YS, Kim DO (2015) Effects of hot air drying on the antioxidant capacity of Actinidia arguta$\times$A. deliciosa cv. Mansoo, a hardy kiwifruit. Korean J Food Sci Technol, 47, 539-543 https://doi.org/10.9721/KJFST.2015.47.4.539
  38. Apak R, Guclu K, Demirata B, Ozyurek M, Esin CS, Bektasoglu B, Berker KI, Ozyur D (2007) Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12, 1496-1547 https://doi.org/10.3390/12071496
  39. Martinez MV, Whitaker JR (1995) The biochemistry and control of enzymatic browning. Trends Food Sci Technol, 6, 195-200 https://doi.org/10.1016/S0924-2244(00)89054-8
  40. Sulaiman A, Soo MJ, Farid M, Silva FVM (2015) Thermosonication for polyphenoloxidase inactivation in fruits: Modeling the ultrasound and thermal kinetics in pear, apple and strawberry purees at different temperatures. J Food Eng, 165, 133-140 https://doi.org/10.1016/j.jfoodeng.2015.06.020
  41. Madamba PS (2002) The response surface methodology: an application to optimize dehydration operations of selected agricultural crops. LWT-Food Sci Technol, 35, 584-592 https://doi.org/10.1016/S0023-6438(02)90914-X