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Matrix metalloproteinase-1 suppression and type-1 procollagen synthesis promoting effects of Uncaria gambir

아선약 추출물의 matrix metalloproteinase-1(MMP-1) 억제 및 제1형 프로콜라겐 합성 촉진 활성

  • Lee, Jaemin (Department of Oral Pathology, School of Dentistry, Kyungpook National University) ;
  • Kim, Dong-Hee (National Development Institute of Korean Medicine) ;
  • Kim, Eun-Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kwon, Hyun Ju (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim, Byung Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Cho, Jae-Hyon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Hyun-Joo (Crop Post-harvest Technology Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Tae Hoon (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
  • 이재민 (경북대학교 치의과학과) ;
  • 김동희 (한약진흥재단) ;
  • 이은우 (동의대학교 생명응용학과) ;
  • 권현주 (동의대학교 생명응용학과) ;
  • 김병우 (동의대학교 생명응용학과) ;
  • 조재현 (경상대학교 수의학과, 동물의학연구원) ;
  • 김현주 (농촌진흥청 국립식량과학원 수확후이용과) ;
  • 김태훈 (동의대학교 블루바이오 소재개발 및 실용화 지원센터)
  • Received : 2016.07.27
  • Accepted : 2016.10.26
  • Published : 2017.02.28

Abstract

Collagen synthesis is decreased and matrix metalloproteinase-1 (MMP-1) levels are increased in naturally aged human skin, and these alterations cause changes such as skin wrinkling and decreased elasticity. As a part of our ongoing search for bioactive ingredients, MMP-1 inhibitory and type-1 procollagen synthesis inducing activities of aqueous methanolic extract of manufactured gambir product from Uncaria gambir were investigated in in vitro bioassay systems. In addition, total phenolic contents were quantified using a spectrophotometric method. Among tested samples, 40% MeOH eluate from 80% methanolic extract of manufactured U. gambir using open column chromatography packed with Diaion HP-20 resin showed significant MMP-1 inhibitory activities with an $IC_{50}$ value of $15.6{\pm}1.3{\mu}g/mL$. Furthermore, type-1 procollagen synthesis promoting property of 40% MeOH eluate ($IC_{50}$ value; $6.9{\pm}0.7{\mu}g/mL$) from 80% methanolic extract of manufactured gambir was higher than other eluates. Additionally, the present investigation revealed that 40% MeOH eluate of manufactured gambir product contained a high level of total phenolic compounds. The result suggests a distinct relationship between anti-wrinkle activity and total phenolic contents, and manufactured gambir product could be considered a new effective source of natural bioactive ingredients. Systematic investigation of manufactured gambir product will be performed for further development of its biological properties.

건조한 아선약을 80% 메탄올로 침지 추출하여 얻어진 추출물에 대해 Diaion HP-20 수지를 충진제로 사용한 칼럼크로마토그래피를 수해하여 6종의 용리액을 얻었으며, 얻어진 추출물에 대하여 MMP-1 저해 및 type-1 procollagen 합성 촉진 활성을 평가하였다. 먼저 MMP-1 저해활성은 총페놀성 화합물의 함량이 상대적으로 높은 40% 메탄올 용리액에서 $IC_{50}$값이 $15.6{\pm}1.3{\mu}g/mL$으로 positive control로 녹차의 주름개선 성분인 (-)-EGCG의 $IC_{50}$값인 $38.4{\pm}2.3{\mu}g/mL$ 보다 우수한 활성이었다. 또한 type-1 procollagen 합성촉진 활성은 총페놀성 화합물 함량이 가장 높은 40% 메탄올 용리액에서 우수한 $EC_{50}$값이 $6.9{\pm}0.7{\mu}g/mL$을 나타내었으며, 양성대조구인 (-)-EGCG의 효능과 동등한 활성이었다. 본 실험에 사용된 아선약 시료의 경우 다양한 화합물이 공존하는 추출물 및 용리액 상태이며 이들 시료에 우수한 효능의 성분의 존재 및 추출물에 존재하는 화합물의 우수한 상승효과의 가능성을 시사하였다. 향후 각종 칼럼크로마토 그래피를 활용한 이들 주름개선 효능 관련 물질의 분리를 통한 활성물질의 구조 결정 및 활성 기작에 대한 연구를 수행할 예정이며, 본 연구결과는 천연물 유래의 우수한 MMP-1 저해 및 type-1 procollagen 합성 촉진 활성을 가지는 새로운 천연 소재 발굴을 위한 기초자료로 활용 가능할 것으로 사료된다.

Keywords

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