Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Aflatoxin B1 Detoxification by Aspergillus oryzae from Meju, a Traditional Korean Fermented Soybean Starter

  • Lee, Kyu Ri (Department of Integrated Biomedical and Life Science, Korea University) ;
  • Yang, Sun Min (Department of Integrated Biomedical and Life Science, Korea University) ;
  • Cho, Sung Min (Department of Integrated Biomedical and Life Science, Korea University) ;
  • Kim, Myunghee (Department of Food Science and Technology, Yeungnam University) ;
  • Hong, Sung-Yong (Department of Integrated Biomedical and Life Science, Korea University) ;
  • Chung, Soo Hyun (Department of Integrated Biomedical and Life Science, Korea University)
  • Received : 2016.07.28
  • Accepted : 2016.10.28
  • Published : 2017.01.28
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Abstract

Aflatoxins are classified as Group 1 (carcinogenic to humans) by the International Agency for Research on Cancer. In this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin $B_1$ ($AFB_1$)-biodetoxification fungi. These fungi were identified as Aspergillus oryzae MAO103 and A. oryzae MAO104 by sequencing the beta-tubulin gene. The two A. oryzae strains were able to degrade more than 90% of $AFB_1$ (initial concentration: $40{\mu}g/l$) in a culture broth in 14 days. The mutagenic effects of $AFB_1$ treated with A. oryzae MAO103 and MAO104 significantly decreased to 5.7% and 6.4%, respectively, in the frame-shift mutation of Ames tests using Salmonella typhimurium TA98. The base-substituting mutagenicity of $AFB_1$ was also decreased by the two fungi. Moreover, $AFB_1$ production by Aspergillus flavus was significantly decreased by the two A. oryzae strains on soybean-based agar plates. Our data suggest that the two $AFB_1$-detoxifying A. oryzae strains have potential application to control $AFB_1$ in foods and feeds.

Keywords

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