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Effects of Cudrania Tricuspidata Root Extract (CTE) on Ethanol-Induced Hangover via Modulating Alcohol Metabolizing Enzyme Activities and Blood Gas Levels in Rats

꾸지뽕나무 뿌리 추출물의 알코올 대사 효소 활성 및 혈액 산성화 기전 조절을 통한 숙취해소 효과

  • Choi, Na-Eun (Department of Biomedical Laboratory Science, Konyang University) ;
  • Ro, Ju-Ye (Department of Biomedical Laboratory Science, Konyang University) ;
  • Lee, Ju-Yeong (Department of Biomedical Laboratory Science, Konyang University) ;
  • Ryu, Jin-Hyeob (Department of Biomedical Laboratory Science, Konyang University) ;
  • Cho, Hyun-Jeong (Department of Biomedical Laboratory Science, Konyang University)
  • 최나은 (건양대학교 임상병리학과) ;
  • 노주예 (건양대학교 임상병리학과) ;
  • 이주영 (건양대학교 임상병리학과) ;
  • 류진협 (건양대학교 임상병리학과) ;
  • 조현정 (건양대학교 임상병리학과)
  • Received : 2016.12.05
  • Accepted : 2017.02.03
  • Published : 2017.02.28

Abstract

To investigate the anti-hangover effects of Cudrania tricuspidata root extract (CTE), the blood alcohol metabolism and blood gas imbalance of CTE in rats treated with 10 ml/kg alcohol were examined. CTE (500 mg/kg and 750 mg/kg) was administrated after 30 minutes of alcohol consumption (10 ml/kg). Blood collection was implemented from the tails of the animals after 1, 3, and 5 hours post alcohol consumption. The Condition drink (a commercial anti-hangover beverage) was used as a positive control. Single administration by the oral route was performed. The consumption of CTE (500 mg/kg and 750 mg/kg) decreased the serum alcohol concentration by increasing and maintaining both the alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) enzyme activity levels in the blood and liver. In addition, CTE led to recovery from the imbalances in the blood gas levels, including carbon dioxide ($CO_2$) and changes in pH, bicarbonate ($HCO_3{^-}$) and lactic acid levels due to alcohol ingestion. In conclusion, CTE exerted a more pronounced anti-hangover effect than a commercial anti-hangover drink. Therefore, CTE can be a novel and safe anti-hangover natural product agent for the prevention or treatment of symptoms caused by excessive alcohol consumption.

본 연구에서는 알코올을 섭취한 흰쥐에서 알코올 대사와 혈액가스 불균형에 대한 꾸지뽕 나무 뿌리 추출물 (이하 CTE)의 효과를 확인하고자 하였다. 알코올을 10 ml/kg의 농도로 흰쥐에게 경구 투여하였고, 섭취 30분 후 CTE를 500 mg/kg 및 750 mg/kg의 농도로 투여하였다. 혈액은 알코올 섭취 1시간, 3시간, 5시간 후 꼬리 정맥을 통하여 채취하였다. 양성 대조군은 시판되고 있는 숙취해소 음료 (컨디션)를 사용하였다. 약물 처리는 모두 단회 경구 투여로 진행되었다. CTE의 섭취는 흰쥐의 혈액과 간 조직 내 알코올 대사 효소인 알코올 분해효소 (alcohol dehydrogenase)와 아세트알데히드 분해효소(acetaldehyde dehydrogenase)를 증가 또는 유지시킴으로써 혈중 알코올 농도를 감소시켰다. 또한, CTE는 알코올섭취에 의한 혈액가스 (pH, $CO_2$, $HCO_3{^-}$, lactic acid)의 불균형을 회복시켰다. 결론적으로, CTE는 현재 시판되고 있는 숙취해소음료 보다 우수한 숙취 해소 효과를 나타내었다. 따라서 본 연구자는 CTE를 숙취 해소 및 예방을 위한 천연물 유래의 안전하고 새로운 물질로써 제시하는 바이다.

Keywords

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