DOI QR코드

DOI QR Code

Effect of Prostaglandins on in vitro Oocyte Final Maturation (GVBD) and Ovulation in the Longchin Goby Chasmichthys dolichognathus

점망둑(Chasmichthys dolichognathus)의 최종성숙(GVBD)과 배란 유도에 미치는 Prostaglandins의 영향

  • Kim, Hyo Eun (Department of Marine Biology, Pukyong National University) ;
  • Baek, Hea Ja (Department of Marine Biology, Pukyong National University)
  • 김효은 (부경대학교 자원생물학과) ;
  • 백혜자 (부경대학교 자원생물학과)
  • Received : 2017.01.13
  • Accepted : 2017.02.07
  • Published : 2017.02.28

Abstract

Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin $E_2$ ($PGE_2$) and prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus. Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with $PGE_2$ or $PGF_{2{\alpha}}$ at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL $PGE_2$ compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL $PGE_2$ or 5, 50, or 500 ng/mL $PGF_{2{\alpha}}$). Neither of the prostaglandins induced ovulation at the concentrations tested.These results suggest that GVBD was induced by incubation with 50 ng/mL $PGE_2$.

Keywords

References

  1. Baek HJ. 2008. Production of C21-steroids in longchin goby Chamichthys dolichognathus during oocyte maturation. Dev Reprod 12, 107-112.
  2. Baek HJ and Lee TY. 1985. Experimental studies on the machanism of reproductive cycle in the longchin goby Chamichthys dolichognathus. Bull Kor Fish Soc 18, 243-252.
  3. Chourasia T and Joy KP. 2012. Role of catechol estrogens on ovarian prostaglandin secretion in vitro in the catfish, Heteropneustes fossilis and possible mechanism of regulation. General Comp Endocrinol 177, 128-142. http://dx.doi.org.10.1016/j.ygcen.2012.03.0003
  4. Goetz FW. 1983. Hormonal control of oocyte final maturation and ovulation in fishes. Fish Physiol 9(Part B), 117-170.
  5. Goetz FW, Duman P, Ranjan M and Herman CA. 1989. Prostaglandin F and E synthesis by specific tissue components of the brook trout Salvelinus fontinalis ovary. J Exp Zool 250, 196-205. https://doi.org/10.1002/jez.1402500211
  6. Goetz FW and Theofan G. 1979. In vitro stimulation of germinal vesicle breakdown and ovulation of yellow perch Perca flavescens oocytes: effects of 17${\alpha}$-hydroxy-20${\beta}$-dihydroprogesterone and prostaglandins. General Comp Endocrinol 37, 273-285. https://doi.org/10.1016/0016-6480(79)90001-7
  7. Joy KP and Singh V. 2013. Functional interactions between vasotocin and prostaglandins during final oocyte maturation and ovulation in the catfish Heteropneustes fossilis. General Comp Endocrinol 186, 126-135. http://dx.doi.org/10.1016/j.ygcen.2013.02.043
  8. Kagawa H, Tanaka H, Unuma T, Ohta H, Gen K and Okujawa K. 2003. Role of prostaglandin in the control of ovulation in the Japanese eel Anguilla japonica. Fish Sci 69, 234-241. https://doi.org/10.1046/j.1444-2906.2003.00613.x
  9. Lister AL and Van Der Kraak G. 2008. An investigation into the role of prostaglandins in zebrafish oocyte maturation and ovulation. General Comp Endocrinol 159, 46-57. http://dx.doi.org/10.1016/j.ygcen.2008.07.017
  10. Lubzens E, Young G, Bobe J and Cerda J. 2010. Oogenesis in teleosts: how fish eggs are formed. General Comp Endocrinol 165, 367-389. http://dx.doi.org/10.1016/j.ygcen.2009.05.022
  11. Mercure F and Van Der Kraak GJ. 1996. Mechanisms of action of free arachidonic acid on ovarian steroid production in the goldfish. General Comp Endocrinol 102, 130-140. https://doi.org/10.1006/gcen.1996.0054
  12. Nagahama Y and Yamashita M. 2008. Regulation of oocyte maturation in fish. Dev Growth Differenti 50, S195-S219. http://dx.doi.org/10.1111/j.1440-169x.2008.01019.x
  13. Pang Y and Ge W. 2002. Gonadotropin and activin enhance maturational competence of oocytes in the zebrafish Danio rerio. Biol Rep 66, 259-265. https://doi.org/10.1095/biolreprod66.2.259
  14. Patino R and Sullivan C. 2002. Ovarian follicle growth, maturation, and ovulation in teleost fish. Fish Physiol Biochem 26, 57-70. https://doi.org/10.1023/A:1023311613987
  15. Patino R, Yoshizaki G, Bolamba D and Thomas P. 2003. Role of arachidonic acid and protein kinase C during maturationinducing hormone-dependent meiotic resumption and ovulation in ovarian follicles of atlantic croaker. Biol Rep 68, 516-523. https://doi.org/10.1095/biolreprod.102.009662
  16. Sales KJ and Jabbour HN. 2003. Cyclooxygenase enzymes and prostaglandins in pathology of the endometrium. Rep 126, 559-567. https://doi.org/10.1530/rep.0.1260559
  17. Sorbera LA, Asturiano JF, Carrillo M and Zanuy S. 2001. Effects of polyunsaturated fatty acids and prostaglandins on oocyte maturation in a marine teleost, the European sea bass Dicentrarchus labrax. Biol Rep 64, 382-389. https://doi.org/10.1095/biolreprod64.1.382
  18. Sorensen PW, Hara TJ, Stacey NE and Goetz FW. 1988. F prostaglandins function as potent olfactory stimulants that comprise the postovulatory female sex pheromone in goldfish. Biol Rep 39, 1039-1050. https://doi.org/10.1095/biolreprod39.5.1039
  19. Thomas P, Pinter J and Das S. 2001. Upregulation of the maturation- inducing steroid membrane receptor in spotted seatrout ovaries by gonadotropin during oocyte maturation and its physiological significance. Biol Rep 64, 21-29. https://doi.org/10.1095/biolreprod64.1.21
  20. Wade M, Van Der Kraak G. 1993. The control of PGE and PGF production by goldfish testis in vivo. J Exp Zool 299, 108-115.
  21. Zohar Y, Munoz-Cueto JA, Elizur A and Kah O. 2010. Neuroendocrinology of reproduction in teleost fish. General Comp Endocrinol 165, 438-455. http://dx.doi.org/10.1016/j.ygcen.2009.04.017